FMNL2 (NM_052905) Human Tagged ORF Clone

CAT#: RC219109

FMNL2 (Myc-DDK-tagged)-Human formin-like 2 (FMNL2)

ORF Plasmid: DDK tGFP



  "NM_052905" in other vectors (6)

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CNY 7,608.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal FMNL2 Antibody
    • 100 ug

CNY 4,897.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms FHOD2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC219109 representing NM_052905
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAACGCAGGGAGCATGGATTCGCAGCAGACCGATTTCAGGGCGCACAACGTGCCTTTGAAGCTGC
CGATGCCAGAGCCAGGTGAACTGGAGGAGCGATTTGCCATCGTGCTGAATGCTATGAACCTACCTCCTGA
CAAAGCCAGGTTACTGCGGCAGTATGATAATGAGAAAAAATGGGAACTGATTTGTGATCAGGAACGATTC
CAGGTGAAGAATCCTCCCCATACATACATTCAAAAGCTCAAAGGCTATCTGGATCCAGCTGTAACCAGGA
AGAAATTCAGACGGCGTGTTCAAGAATCTACACAAGTGCTAAGAGAACTGGAAATTTCTTTGAGAACTAA
CCACATTGGATGGGTCAGAGAATTTCTGAATGAAGAAAACAAAGGTCTTGATGTTCTAGTGGAATATCTC
TCATTTGCACAGTACGCGGTAACTTTTGACTTTGAAAGTGTGGAGAGTACTGTGGAGAGCTCGGTGGACA
AATCAAAGCCCTGGAGTAGGTCCATCGAGGACCTGCACAGAGGGAGCAACCTGCCCTCACCTGTGGGCAA
CAGTGTCTCCCGCTCTGGAAGACATTCTGCACTGCGATATAATACATTGCCAAGCAGAAGAACTCTGAAA
AATTCAAGATTAGTGAGTAAGAAAGATGATGTGCATGTCTGTATCATGTGTTTACGTGCCATCATGAATT
ATCAGTATGGTTTCAACATGGTCATGTCTCATCCACACGCTGTCAATGAGATTGCACTAAGCCTGAACAA
CAAGAATCCCAGAACAAAAGCCCTTGTCTTAGAACTGTTGGCAGCCGTTTGTCTTGTCAGAGGCGGGCAT
GAAATCATTTTATCAGCATTTGATAACTTTAAAGAGGTTTGTGGAGAAAAACAGCGCTTTGAGAAGTTGA
TGGAACATTTCAGGAATGAAGACAATAACATAGATTTTATGGTGGCTTCTATGCAGTTTATTAATATTGT
AGTCCATTCAGTAGAAGATATGAATTTCAGAGTTCACCTGCAGTATGAATTTACCAAATTAGGCCTGGAC
GAATACTTGGACAAGCTGAAACACACTGAGAGTGACAAGCTTCAAGTCCAGATCCAGGCTTACCTGGACA
ATGTTTTTGATGTAGGAGCTCTACTGGAAGATGCTGAAACTAAGAATGCTGCCTTGGAGAGGGTGGAAGA
ACTGGAAGAAAACATTTCTCATTTATCTGAAAAACTGCAAGACACAGAGAATGAAGCCATGTCCAAGATT
GTGGAACTGGAAAAGCAACTCATGCAGAGGAACAAGGAGCTGGATGTCGTTCGGGAAATCTACAAAGATG
CAAATACTCAAGTTCACACATTAAGAAAAATGGTCAAAGAAAAAGAAGAAGCAATTCAAAGACAGTCTAC
CCTGGAAAAAAAGATTCATGAGCTAGAGAAACAAGGGACCATTAAAATTCAGAAGAAAGGGGATGGGGAT
ATCGCCATACTGCCAGTTGTGGCTTCTGGCACATTGTCCATGGGGTCAGAAGTGGTAGCAGGTAACTCTG
TGGGACCCACAATGGGGGCCGCTTCCTCAGGACCCTTGCCCCCTCCTCCACCACCACTGCCTCCCTCATC
AGACACACCTGAAACAGTGCAAAATGGTCCAGTAACACCACCTATGCCACCGCCGCCGCCGCCCCCTCCT
CCACCTCCTCCTCCCCCACCGCCCCCTCCGCCTCCTCCTCTCCCAGGCCCTGCAGCTGAGACTGTACCAG
CTCCTCCCTTAGCACCTCCCCTTCCCTCTGCACCTCCGCTGCCTGGAACATCTTCACCCACAGTGGTTTT
CAACTCAGGATTAGCAGCTGTGAAAATTAAGAAGCCAATCAAGACGAAGTTCAGAATGCCAGTGTTTAAC
TGGGTTGCTCTGAAGCCCAATCAGATCAATGGCACAGTCTTCAATGAAATTGATGATGAGCGAATTCTGG
AGGATTTAAATGTGGATGAATTTGAGGAAATATTCAAGACAAAAGCCCAAGGACCTGCCATTGATCTTTC
TTCAAGCAAACAGAAGATACCACAGAAGGGATCAAACAAAGTGACATTACTAGAAGCAAACAGGGCCAAA
AATCTTGCCATAACTTTAAGGAAAGCTGGAAAGACTGCTGATGAAATATGTAAAGCTATTCATGTATTTG
ACTTGAAGACACTGCCTGTGGACTTTGTGGAATGCTTGATGCGGTTCCTACCAACTGAGAATGAAGTGAA
AGTGCTTCGGCTCTACGAGCGGGAAAGGAAGCCTCTGGAAAACTTGTCAGATGAAGATCGGTTCATGATG
CAGTTTAGTAAAATCGAGAGGCTCATGCAGAAGATGACCATCATGGCCTTCATTGGGAACTTTGCTGAAA
GCATTCAGATGCTGACTCCTCAACTACATGCGATTATAGCAGCATCTGTCTCTATAAAGTCGTCCCAAAA
ACTCAAGAAAATTCTGGAGATCATCTTAGCCCTTGGAAACTACATGAATAGCAGTAAAAGAGGAGCAGTT
TATGGATTTAAACTTCAGAGTTTAGATCTGCTCTTAGATACAAAGTCAACAGACAGAAAGCAAACACTGT
TGCACTATATATCCAATGTGGTGAAAGAAAAATATCACCAAGTGTCCCTGTTTTATAATGAGCTTCATTA
TGTGGAAAAAGCTGCTGCAGTCTCCCTTGAGAATGTTTTGCTGGATGTCAAGGAGCTCCAGAGGGGAATG
GACTTGACCAAGAGAGAGTACACCATGCATGACCATAACACGCTGCTGAAGGAGTTCATCCTCAACAATG
AGGGGAAGCTGAAGAAGCTGCAGGATGATGCCAAGATCGCACAGGATGCCTTTGATGATGTTGTGAAGTA
TTTTGGAGAAAACCCCAAGACAACACCACCCTCTGTCTTCTTTCCTGTCTTTGTCCGGTTTGTGAAAGCA
TATAAGCAAGCAGAAGAGGAAAATGAGCTGAGGAAAAAGCAGGAACAAGCTCTCATGGAAAAACTCCTAG
AGCAAGAAGCTCTGATGGAGCAGCAGGATCCAAAGTCTCCTTCTCATAAATCAAAGAGGCAGCAGCAAGA
GTTAATTGCAGAATTAAGAAGACGACAAGTTAAAGATAACAGACATGTATATGAGGGAAAAGATGGTGCC
ATTGAAGATATTATCACAGATCTTAGAAACCAACCATACAGACGAGCCGATGCGGTGAGGAGAAGCGTCA
GGCGGCGCTTTGATGATCAGAACTTGCGTTCTGTTAATGGTGCCGAAATAACAATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC219109 representing NM_052905
Red=Cloning site Green=Tags(s)

MGNAGSMDSQQTDFRAHNVPLKLPMPEPGELEERFAIVLNAMNLPPDKARLLRQYDNEKKWELICDQERF
QVKNPPHTYIQKLKGYLDPAVTRKKFRRRVQESTQVLRELEISLRTNHIGWVREFLNEENKGLDVLVEYL
SFAQYAVTFDFESVESTVESSVDKSKPWSRSIEDLHRGSNLPSPVGNSVSRSGRHSALRYNTLPSRRTLK
NSRLVSKKDDVHVCIMCLRAIMNYQYGFNMVMSHPHAVNEIALSLNNKNPRTKALVLELLAAVCLVRGGH
EIILSAFDNFKEVCGEKQRFEKLMEHFRNEDNNIDFMVASMQFINIVVHSVEDMNFRVHLQYEFTKLGLD
EYLDKLKHTESDKLQVQIQAYLDNVFDVGALLEDAETKNAALERVEELEENISHLSEKLQDTENEAMSKI
VELEKQLMQRNKELDVVREIYKDANTQVHTLRKMVKEKEEAIQRQSTLEKKIHELEKQGTIKIQKKGDGD
IAILPVVASGTLSMGSEVVAGNSVGPTMGAASSGPLPPPPPPLPPSSDTPETVQNGPVTPPMPPPPPPPP
PPPPPPPPPPPPPLPGPAAETVPAPPLAPPLPSAPPLPGTSSPTVVFNSGLAAVKIKKPIKTKFRMPVFN
WVALKPNQINGTVFNEIDDERILEDLNVDEFEEIFKTKAQGPAIDLSSSKQKIPQKGSNKVTLLEANRAK
NLAITLRKAGKTADEICKAIHVFDLKTLPVDFVECLMRFLPTENEVKVLRLYERERKPLENLSDEDRFMM
QFSKIERLMQKMTIMAFIGNFAESIQMLTPQLHAIIAASVSIKSSQKLKKILEIILALGNYMNSSKRGAV
YGFKLQSLDLLLDTKSTDRKQTLLHYISNVVKEKYHQVSLFYNELHYVEKAAAVSLENVLLDVKELQRGM
DLTKREYTMHDHNTLLKEFILNNEGKLKKLQDDAKIAQDAFDDVVKYFGENPKTTPPSVFFPVFVRFVKA
YKQAEEENELRKKQEQALMEKLLEQEALMEQQDPKSPSHKSKRQQQELIAELRRRQVKDNRHVYEGKDGA
IEDIITDLRNQPYRRADAVRRSVRRRFDDQNLRSVNGAEITM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_052905
ORF Size 3276 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_052905.4
RefSeq Size 5591 bp
RefSeq ORF 3279 bp
Locus ID 114793
UniProt ID Q96PY5
Protein Families Transcription Factors
MW 123.9 kDa
Gene Summary This gene encodes a formin-related protein. Formin-related proteins have been implicated in morphogenesis, cytokinesis, and cell polarity. Alternatively spliced transcript variants encoding different isoforms have been described but their full-length nature has yet to be determined. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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