TAF1 (NM_004606) Human Tagged ORF Clone
CAT#: RC219444
- TrueORF®
TAF1 (Myc-DDK-tagged)-Human TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant 1
ORF Plasmid: tGFP
"NM_004606" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 19,088.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | BA2R; CCG1; CCGS; DYT3; DYT3/TAF1; KAT4; MRXS33; N-TAF1; NSCL2; OF; P250; TAF(II)250; TAF2A; TAFII-250; TAFII250; XDP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC219444 representing NM_004606
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACCCGGCTGCGATTTGCTGCTGCGGACAGCAGCTACCATCACTGCTGCCGCCATCATGTCAGACA CGGACAGCGACGAAGATTCCGCTGGAGGCGGCCCATTTTCTTTAGCGGGTTTCCTTTTCGGCAACATCAA TGGAGCCGGGCAGCTGGAGGGGGAAAGCGTCTTGGATGATGAATGTAAGAAGCACTTGGCAGGCTTGGGG GCTTTGGGGCTGGGCAGCCTGATCACTGAACTCACGGCAAATGAAGAATTGACCGGGACTGACGGTGCCT TGGTAAATGATGAAGGGTGGGTTAGGAGTACAGAAGATGCTGTGGACTATTCAGACATCAATGAGGTGGC AGAAGATGAAAGCCGAAGATACCAGCAGACGATGGGGAGCTTGCAGCCCCTTTGCCACTCAGATTATGAT GAAGATGACTATGATGCTGATTGTGAAGACATTGATTGCAAGTTGATGCCTCCTCCACCTCCACCCCCGG GACCAATGAAGAAGGATAAGGACCAGGATTCTATTACTGGTGTGTCTGAAAATGGAGAAGGCATCATCTT GCCCTCCATCATTGCCCCTTCCTCTTTGGCCTCAGAGAAAGTGGACTTCAGTAGTTCCTCTGACTCAGAA TCTGAGATGGGACCTCAGGAAGCAACACAGGCAGAATCTGAAGATGGAAAGCTGACCCTTCCATTGGCTG GGATTATGCAGCATGATGCCACCAAGCTGTTGCCAAGTGTCACAGAACTTTTTCCAGAATTTCGACCTGG AAAGGTGTTACGTTTTCTACGTCTTTTTGGACCAGGGAAGAATGTCCCATCTGTTTGGCGGAGTGCTCGG AGAAAGAGGAAGAAGAAGCACCGTGAGCTGATACAGGAAGAGCAGATCCAGGAGGTGGAGTGCTCAGTAG AATCAGAAGTCAGCCAGAAGTCTTTGTGGAACTACGACTACGCTCCACCACCACCTCCAGAGCAGTGTCT CTCTGATGATGAAATCACGATGATGGCTCCTGTGGAGTCCAAATTTTCCCAATCAACTGGAGATATAGAT AAAGTGACAGATACCAAACCAAGAGTGGCTGAGTGGCGTTATGGGCCTGCCCGACTGTGGTATGATATGC TGGGTGTCCCTGAAGATGGCAGTGGGTTTGACTATGGCTTCAAACTGAGAAAGACAGAACATGAACCTGT GATAAAATCTAGAATGATAGAGGAATTTAGGAAACTTGAGGAAAACAATGGCACTGATCTTCTGGCTGAT GAAAACTTCCTGATGGTGACACAGCTGCATTGGGAGGATGATATCATCTGGGATGGGGAGGATGTCAAAC ACAAAGGGACAAAACCTCAGCGTGCAAGCCTGGCAGGCTGGCTTCCTTCTAGCATGACTAGGAATGCGAT GGCTTACAATGTTCAGCAAGGTTTTGCAGCCACTCTTGATGATGACAAACCTTGGTACTCCATTTTTCCC ATTGACAATGAGGATCTGGTATATGGACGCTGGGAGGACAATATCATTTGGGATGCTCAGGCCATGCCCC GGCTGTTGGAACCTCCTGTTTTGACACTTGATCCCAATGATGAGAACCTCATTTTGGAAATTCCTGATGA GAAGGAAGAGGCCACCTCTAACTCCCCCTCCAAGGAGAGTAAGAAGGAATCATCTCTGAAGAAGAGTCGA ATTCTCTTAGGGAAAACAGGAGTCATCAAGGAGGAACCACAGCAGAACATGTCTCAGCCAGAAGTGAAAG ATCCATGGAATCTCTCCAATGATGAGTATTATTATCCCAAGCAACAGGGTCTTCGAGGCACCTTTGGAGG GAATATTATCCAGCATTCAATTCCTGCTGTGGAATTACGGCAGCCCTTCTTTCCCACCCACATGGGGCCC ATCAAACTCCGGCAGTTCCATCGCCCACCTCTGAAAAAGTACTCATTTGGTGCACTTTCTCAGCCAGGTC CCCACTCAGTCCAACCTTTGCTAAAGCACATCAAAAAAAAGGCCAAGATGAGAGAACAAGAGAGGCAAGC TTCAGGTGGTGGAGAGATGTTTTTTATGCGCACACCTCAGGACCTCACAGGCAAAGATGGTGATCTTATT CTTGCAGAATATAGTGAGGAAAATGGACCCTTAATGATGCAGGTTGGCATGGCAACCAAGATAAAGAACT ATTATAAACGGAAACCTGGAAAAGATCCTGGAGCACCAGATTGTAAATATGGGGAAACTGTTTACTGCCA TACATCTCCTTTCCTGGGTTCTCTCCATCCTGGCCAATTGCTGCAAGCATTTGAGAACAACCTTTTTCGT GCTCCAATTTATCTTCATAAGATGCCAGAAACTGATTTCTTGATCATTCGGACAAGACAGGGTTACTATA TTCGGGAATTAGTGGATATTTTTGTGGTTGGCCAGCAGTGTCCCTTGTTTGAAGTTCCTGGGCCTAACTC CAAAAGGGCCAATACGCATATTCGAGACTTTCTACAGGTTTTTATTTACCGCCTTTTCTGGAAAAGTAAA GATCGGCCACGGAGGATACGAATGGAAGATATAAAAAAAGCCTTTCCTTCCCATTCAGAAAGCAGCATCC GGAAGAGGCTAAAGCTCTGCGCTGACTTCAAACGCACAGGGATGGACTCAAACTGGTGGGTGCTTAAGTC TGATTTTCGTTTACCAACGGAAGAAGAGATCAGAGCTATGGTGTCACCAGAGCAGTGCTGTGCTTATTAT AGCATGATAGCTGCAGAGCAACGACTGAAGGATGCTGGCTATGGTGAGAAATCCTTTTTTGCTCCAGAAG AAGAAAATGAGGAAGATTTCCAGATGAAGATTGATGATGAAGTTCGCACTGCCCCTTGGAACACCACAAG GGCCTTCATTGCTGCCATGAAGGGCAAGTGTCTGCTAGAGGTGACTGGGGTGGCAGATCCCACGGGGTGT GGTGAAGGATTCTCCTATGTGAAGATTCCAAACAAACCAACACAGCAGAAGGATGATAAAGAACCGCAGC CAGTGAAGAAGACAGTGACAGGAACAGATGCAGACCTTCGTCGCCTTTCCCTGAAAAATGCCAAGCAACT TCTACGTAAATTTGGTGTGCCTGAGGAAGAGATTAAAAAGTTGTCCCGCTGGGAAGTGATTGATGTGGTG CGCACAATGTCAACAGAACAGGCTCGTTCTGGAGAGGGGCCCATGAGTAAATTTGCCCGTGGATCAAGGT TTTCTGTGGCTGAGCATCAAGAGCGTTACAAAGAGGAATGTCAGCGCATCTTTGACCTACAGAACAAGGT TCTGTCATCAACTGAAGTCTTATCAACTGACACAGACAGCAGCTCAGCTGAAGATAGTGACTTTGAAGAA ATGGGAAAGAACATTGAGAACATGTTGCAGAACAAGAAAACCAGCTCTCAGCTTTCACGTGAACGGGAGG AACAGGAGCGGAAGGAACTACAGCGAATGCTACTGGCAGCAGGCTCAGCAGCATCCGGAAACAATCACAG AGATGATGACACAGCTTCCGTGACTAGCCTTAACTCTTCTGCCACTGGACGCTGTCTCAAGATTTATCGC ACGTTTCGAGATGAAGAGGGGAAAGAGTATGTTCGCTGTGAGACAGTCCGAAAACCAGCTGTCATTGATG CCTATGTGCGCATACGGACTACAAAAGATGAGGAATTCATTCGAAAATTTGCCCTTTTTGATGAACAACA TCGGGAAGAGATGCGAAAAGAACGGCGGAGGATTCAAGAGCAACTGAGGCGGCTTAAGAGGAACCAGGAA AAGGAGAAGCTTAAGGGTCCTCCTGAGAAGAAGCCCAAGAAAATGAAGGAGCGTCCTGACCTAAAACTGA AATGTGGGGCATGTGGTGCCATTGGACACATGAGGACTAACAAATTCTGCCCCCTCTATTATCAAACAAA TGCGCCACCTTCCAACCCTGTTGCCATGACAGAAGAACAGGAGGAGGAGTTGGAAAAGACAGTCATTCAT AATGATAATGAAGAACTTATCAAGGTTGAAGGGACCAAAATTGTCTTGGGGAAACAGCTAATTGAGAGTG CGGATGAGGTTCGCAGAAAATCTCTGGTTCTCAAGTTTCCTAAACAGCAGCTTCCTCCAAAGAAGAAACG GCGAGTTGGAACCACTGTTCACTGTGACTATTTGAATAGACCTCATAAGTCCATCCACCGGCGCCGCACA GACCCTATGGTGACGCTGTCGTCCATCTTGGAGTCTATCATCAATGACATGAGAGATCTTCCAAATACAT ACCCTTTCCACACTCCAGTCAATGCAAAGGTTGTAAAGGACTACTACAAAATCATCACTCGGCCAATGGA CCTACAAACACTCCGCGAAAACGTGCGTAAACGCCTCTACCCATCTCGGGAAGAGTTCAGAGAGCATCTG GAGCTAATTGTGAAAAATAGTGCAACCTACAATGGGCCAAAACACTCATTGACTCAGATCTCTCAATCCA TGCTGGATCTCTGTGATGAAAAACTCAAAGAGAAAGAAGACAAATTAGCTCGCTTAGAGAAAGCTATCAA CCCCTTGCTGGATGATGATGACCAAGTGGCGTTTTCTTTCATTCTGGACAACATTGTCACCCAGAAAATG ATGGCAGTTCCAGATTCTTGGCCATTTCATCACCCAGTTAATAAGAAATTTGTTCCAGATTATTACAAAG TGATTGTCAATCCAATGGATTTAGAGACCATACGTAAGAACATCTCCAAGCACAAGTATCAGAGTCGGGA GAGCTTTCTGGATGATGTAAACCTTATTCTGGCCAACAGTGTTAAGTATAATGGACCTGAGAGTCAGTAT ACTAAGACTGCCCAGGAGATTGTGAACGTCTGTTACCAGACATTGACTGAGTATGATGAACATTTGACTC AACTTGAGAAGGATATTTGTACTGCTAAAGAAGCAGCTTTGGAGGAAGCAGAATTAGAAAGCCTGGACCC AATGACCCCAGGGCCCTACACGCCTCAGCCTCCTGATTTGTATGATACCAACACATCCCTCAGTATGTCT CGAGATGCCTCTGTATTTCAAGATGAGAGCAATATGTCTGTCTTGGATATTCCCAGTGCCACTCCAGAAA AGCAGGTAACACAGGAAGGTGAAGATGGAGATGGTGATCTTGCAGATGAAGAGGAAGGAACTGTACAACA GCCTCAAGCCAGTGTCCTGTATGAGGATTTGCTTATGTCTGAAGGAGAAGATGATGAGGAAGATGCTGGG AGTGATGAAGAAGGAGACAATCCTTTCTCTGCTATCCAGCTGAGTGAAAGTGGAAGTGACTCTGATGTGG GATCTGGTGGAATAAGACCCAAACAACCCCGCATGCTTCAGGAGAACACAAGGATGGACATGGAAAATGA AGAAAGCATGATGTCCTATGAGGGAGACGGTGGGGAGGCTTCCCATGGTTTGGAGGATAGCAACATCAGT TATGGGAGCTATGAGGAGCCTGATCCCAAGTCGAACACCCAAGACACAAGCTTCAGCAGCATCGGTGGGT ATGAGGTATCAGAGGAGGAAGAAGATGAGGAGGAGGAAGAGCAGCGCTCTGGGCCGAGCGTACTAAGCCA GGTCCACCTGTCAGAGGACGAGGAGGACAGTGAGGATTTCCACTCCATTGCTGGGGACAGTGACTTGGAC TCTGATGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219444 representing NM_004606
Red=Cloning site Green=Tags(s) MGPGCDLLLRTAATITAAAIMSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLG ALGLGSLITELTANEELTGTDGALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHSDYD EDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDSITGVSENGEGIILPSIIAPSSLASEKVDFSSSSDSE SEMGPQEATQAESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSAR RKRKKKHRELIQEEQIQEVECSVESEVSQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDID KVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKTEHEPVIKSRMIEEFRKLEENNGTDLLAD ENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFP IDNEDLVYGRWEDNIIWDAQAMPRLLEPPVLTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSR ILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGP IKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLI LAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFR APIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSK DRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYY SMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGC GEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVV RTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEVLSTDTDSSSAEDSDFEE MGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYR TFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQE KEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIH NDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRT DPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHL ELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKM MAVPDSWPFHHPVNKKFVPDYYKVIVNPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQY TKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMS RDASVFQDESNMSVLDIPSATPEKQVTQEGEDGDGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAG SDEEGDNPFSAIQLSESGSDSDVGSGGIRPKQPRMLQENTRMDMENEESMMSYEGDGGEASHGLEDSNIS YGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDSDLD SDE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_004606 |
ORF Size | 5679 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004606.4 |
RefSeq Size | 6137 bp |
RefSeq ORF | 5622 bp |
Locus ID | 6872 |
UniProt ID | P21675 |
Protein Families | Protein Kinase |
Protein Pathways | Basal transcription factors |
MW | 214.5 kDa |
Gene Summary | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N- and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Mutations in this gene result in Dystonia 3, torsion, X-linked, a dystonia-parkinsonism disorder. Alternative splicing of this gene results in multiple transcript variants. This gene is part of a complex transcription unit (TAF1/DYT3), wherein some transcript variants share exons with TAF1 as well as additional downstream DYT3 exons. [provided by RefSeq, Oct 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC219444L1 | Lenti ORF clone of Human TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant 1, Myc-DDK-tagged |
CNY 21,488.00 |
|
RG219444 | TAF1 (tGFP-tagged) - Human TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant 1 |
CNY 20,810.00 |
|
SC303505 | TAF1 (untagged)-Human TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant 1 |
CNY 36,390.00 |