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ADH6 (NM_001102470) Human Tagged ORF Clone
CAT＃: RC219515
ADH6 (Myc-DDK-tagged)-Human alcohol dehydrogenase 6 (class V) (ADH6), transcript variant 1
ORF Plasmid: tGFP
"NM_001102470" in other vectors (4)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ADH-5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219515 representing NM_001102470
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTACTACAGGCCAAGTCATCAGATGCAAAGCAGCCATACTCTGGAAGCCTGGTGCACCATTTTCTA TTGAAGAGGTAGAAGTGGCCCCACCAAAGGCAAAGGAAGTTCGCATAAAGGTTGTGGCCACCGGACTGTG TGGTACAGAGATGAAAGTGTTGGGGAGTAAACACTTGGACCTCTTGTATCCCACCATCTTGGGCCATGAA GGGGCTGGAATCGTTGAGAGTATTGGAGAAGGAGTAAGCACAGTGAAACCAGGTGACAAAGTTATCACAC TCTTTCTGCCACAGTGTGGAGAATGTACCTCTTGCCTGAATTCTGAGGGCAATTTTTGTATACAATTCAA ACAGTCAAAAACCCAACTGATGTCTGATGGTACCAGCAGGTTTACCTGCAAGGGAAAATCAATATATCAC TTTGGTAATACCAGCACCTTCTGTGAATACACAGTGATAAAGGAAATCTCAGTTGCCAAGATTGATGCAG TCGCTCCTCTAGAGAAAGTATGCCTAATTAGCTGTGGCTTTTCCACTGGGTTTGGTGCTGCAATCAATAC TGCCAAGGTGACTCCAGGTTCTACCTGTGCTGTGTTTGGCCTGGGAGGAGTCGGCTTGTCTGTTGTCATG GGTTGTAAAGCAGCAGGAGCAGCCAGGATCATTGGAGTGGATGTCAACAAGGAGAAATTTAAGAAGGCAC AGGAATTGGGTGCTACTGAGTGCCTCAACCCTCAGGACTTAAAGAAACCCATTCAAGAAGTTTTATTTGA TATGACAGATGCTGGTATAGACTTCTGCTTTGAGGCCATTGGAAATCTGGACGTTCTGGCAGCTGCCCTC GCCTCCTGCAATGAGAGCTATGGGGTCTGTGTGGTTGTTGGGGTGTTGCCTGCCAGTGTTCAACTCAAAA TCAGTGGCCAGTTGTTCTTCTCAGGACGTTCTTTGAAGGGTTCTGTTTTTGGAGGCTGGAAGAGCAGACA GCACATCCCTAAACTGGTTGCTGATTATATGGCAGAGAAGTTGAATCTAGATCCACTAATTACTCATACT CTGAATCTTGATAAAATCAATGAAGCAGTTGAATTAATGAAAACTGGAAAATGTATCCGCTGTATCCTGT TACTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219515 representing NM_001102470
Red=Cloning site Green=Tags(s) MSTTGQVIRCKAAILWKPGAPFSIEEVEVAPPKAKEVRIKVVATGLCGTEMKVLGSKHLDLLYPTILGHE GAGIVESIGEGVSTVKPGDKVITLFLPQCGECTSCLNSEGNFCIQFKQSKTQLMSDGTSRFTCKGKSIYH FGNTSTFCEYTVIKEISVAKIDAVAPLEKVCLISCGFSTGFGAAINTAKVTPGSTCAVFGLGGVGLSVVM GCKAAGAARIIGVDVNKEKFKKAQELGATECLNPQDLKKPIQEVLFDMTDAGIDFCFEAIGNLDVLAAAL ASCNESYGVCVVVGVLPASVQLKISGQLFFSGRSLKGSVFGGWKSRQHIPKLVADYMAEKLNLDPLITHT LNLDKINEAVELMKTGKCIRCILLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001102470 |
| ORF Size | 1125 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001102470.2 |
| RefSeq Size | 2803 bp |
| RefSeq ORF | 1128 bp |
| Locus ID | 130 |
| UniProt ID | P28332 |
| Protein Families | Druggable Genome |
| Protein Pathways | Drug metabolism - cytochrome P450, Fatty acid metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism, Tyrosine metabolism |
| MW | 39.6 kDa |
| Gene Summary | This gene encodes class V alcohol dehydrogenase, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This gene is expressed in the stomach as well as in the liver, and it contains a glucocorticoid response element upstream of its 5' UTR, which is a steroid hormone receptor binding site. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219515L3 | Lenti ORF clone of Human alcohol dehydrogenase 6 (class V) (ADH6), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC219515L4 | Lenti ORF clone of Human alcohol dehydrogenase 6 (class V) (ADH6), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG219515 | ADH6 (tGFP-tagged) - Human alcohol dehydrogenase 6 (class V) (ADH6), transcript variant 1 |
CNY 4,370.00 |
|
| SC316928 | ADH6 (untagged)-Human alcohol dehydrogenase 6 (class V) (ADH6), transcript variant 1 |
CNY 5,488.00 |
