SAP155 (SF3B1) (NM_012433) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Hsh155; MDS; PRP10; PRPF10; SAP155; SF3b155
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC219605 representing NM_012433, codon optimized. Due to the complexity of NM_012433, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGAAGATCGCCAAGACTCACGAAGATATTGAAGCACAGATTCGAGAAATTCAAGGCAAGAAGGCA GCTCTTGATGAAGCTCAAGGAGTGGGCCTCGATTCTACAGGTTATTATGACCAGGAAATTTATGGTGGA AGTGACAGCAGATTTGCTGGATACGTGACATCAATTGCTGCAACTGAACTTGAAGATGATGACGATGAC TATTCATCATCTACGAGTTTGCTTGGTCAGAAGAAGCCAGGATATCATGCCCCTGTGGCATTGCTTAAT GATATACCACAGTCAACAGAACAGTATGATCCATTTGCTGAGCACAGACCTCCAAAGATTGCAGACCGG GAAGATGAATACAAAAAGCATAGGCGGACCATGATAATTTCCCCAGAGCGTCTTGATCCTTTTGCAGAT GGAGGGAAAACCCCTGATCCTAAAATGAATGCTAGGACTTACATGGATGTAATGCGAGAACAACACTTG ACTAAAGAAGAACGAGAAATTAGGCAACAGCTAGCAGAAAAAGCTAAAGCTGGAGAACTAAAAGTCGTC AATGGAGCAGCAGCGTCCCAGCCTCCATCAAAACGAAAACGGCGTTGGGATCAAACAGCTGATCAGACT CCTGGTGCCACTCCCAAAAAACTATCAAGTTGGGATCAGGCAGAGACCCCTGGGCATACTCCTTCCTTA AGATGGGATGAGACACCAGGTCGTGCAAAGGGAAGCGAGACTCCTGGAGCAACCCCAGGCTCAAAAATA TGGGATCCTACACCTAGCCACACACCAGCGGGAGCTGCTACTCCTGGACGAGGTGATACACCAGGCCAT GCGACACCAGGCCATGGAGGCGCAACTTCCAGTGCTCGTAAAAACAGATGGGATGAAACCCCCAAAACA GAGAGAGATACTCCTGGGCATGGAAGTGGATGGGCTGAGACTCCTCGAACAGATCGAGGTGGAGATTCT ATTGGTGAAACACCGACTCCTGGAGCCAGTAAAAGAAAATCACGGTGGGATGAAACACCAGCTAGTCAG ATGGGTGGAAGCACTCCAGTTCTGACCCCTGGAAAGACACCAATTGGCACACCAGCCATGAACATGGCT ACCCCTACTCCAGGTCACATAATGAGTATGACTCCTGAACAGCTTCAGGCTTGGCGGTGGGAAAGAGAA ATTGATGAGAGAAATCGCCCACTTTCTGATGAGGAATTAGATGCTATGTTCCCAGAAGGATATAAGGTA CTTCCTCCTCCAGCTGGTTATGTTCCTATTCGAACTCCAGCTCGAAAGCTGACAGCTACTCCAACACCT TTGGGTGGTATGACTGGTTTCCACATGCAAACTGAAGATCGAACTATGAAAAGTGTTAATGACCAGCCA TCTGGAAATCTTCCATTTTTAAAACCTGATGATATTCAATACTTTGATAAACTATTGGTTGATGTTGAT GAATCAACACTTAGTCCAGAAGAGCAAAAAGAGAGAAAAATAATGAAGTTGCTTTTAAAAATTAAGAAT GGAACACCACCAATGAGAAAGGCTGCATTGCGTCAGATTACTGATAAAGCTCGTGAATTTGGAGCTGGT CCTTTGTTTAATCAGATTCTTCCTCTGCTGATGTCTCCTACACTTGAGGATCAAGAGCGTCATTTACTT GTGAAAGTTATTGATAGGATACTGTACAAACTTGATGACTTAGTTCGTCCATATGTGCATAAGATCCTC GTGGTCATTGAACCGCTATTGATTGATGAAGATTACTATGCTAGAGTGGAAGGCCGAGAGATCATTTCT AATTTGGCAAAGGCTGCTGGTCTGGCTACTATGATCTCTACCATGAGACCTGATATAGATAACATGGAT GAGTATGTCCGTAACACAACAGCTAGAGCTTTTGCTGTTGTAGCCTCTGCCCTGGGCATTCCTTCTTTA TTGCCCTTCTTAAAAGCTGTGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGACACACTGGTATTAAGATT GTACAACAGATAGCTATTCTTATGGGCTGTGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATT GAACATGGTCTTGTGGATGAGCAGCAGAAAGTTCGGACCATCAGTGCTTTGGCCATTGCTGCCTTGGCT GAAGCAGCAACTCCTTATGGTATCGAATCTTTTGATTCTGTGTTAAAGCCTTTATGGAAGGGTATCCGC CAACACAGAGGAAAGGGTTTGGCTGCTTTCTTGAAGGCTATTGGGTATCTTATTCCTCTTATGGATGCA GAATATGCCAACTACTATACTAGAGAAGTGATGTTAATCCTTATTCGAGAATTCCAGTCTCCTGATGAG GAAATGAAAAAAATTGTGCTGAAGGTGGTAAAACAGTGTTGTGGGACAGATGGTGTAGAAGCAAACTAC ATTAAAACAGAGATTCTTCCTCCCTTTTTTAAACACTTCTGGCAGCACAGGATGGCTTTGGATAGAAGA AATTACCGACAGTTAGTTGATACTACTGTGGAGTTGGCAAACAAAGTAGGTGCAGCAGAAATTATATCC AGGATTGTGGATGATCTGAAAGATGAAGCCGAACAGTACAGAAAAATGGTGATGGAGACAATTGAGAAA ATTATGGGTAATTTGGGAGCAGCAGATATTGATCATAAACTTGAAGAACAACTGATTGATGGTATTCTT TATGCTTTCCAAGAACAGACTACAGAGGACTCAGTAATGTTGAACGGCTTTGGCACAGTGGTTAATGCT CTTGGCAAACGAGTCAAACCATACTTGCCTCAGATCTGTGGTACAGTTTTGTGGCGTTTAAATAACAAA TCTGCTAAAGTTAGGCAACAGGCAGCTGACTTGATTTCTCGAACTGCTGTTGTCATGAAGACTTGTCAA GAGGAAAAATTGATGGGACACTTGGGTGTTGTATTGTATGAGTATTTGGGTGAAGAGTACCCTGAAGTA TTGGGCAGCATTCTTGGAGCACTGAAGGCCATTGTAAATGTCATAGGTATGCATAAGATGACTCCACCA ATTAAAGATCTGCTGCCTAGACTCACCCCCATCTTAAAGAACAGACATGAAAAAGTACAAGAGAATTGT ATTGATCTTGTTGGTCGTATTGCTGACAGGGGAGCTGAATATGTATCTGCAAGAGAGTGGATGAGGATT TGCTTTGAGCTTTTAGAGCTCTTAAAAGCCCACAAAAAGGCTATTCGTAGAGCCACAGTCAACACATTT GGTTATATTGCAAAGGCCATTGGCCCTCATGATGTATTGGCTACACTTCTGAACAACCTCAAAGTTCAA GAAAGGCAGAACAGAGTTTGTACCACTGTAGCAATAGCTATTGTTGCAGAAACATGTTCACCCTTTACA GTACTCCCTGCCTTAATGAATGAATACAGAGTTCCTGAACTGAATGTTCAAAATGGAGTGTTAAAATCG CTTTCCTTCTTGTTTGAATATATTGGTGAAATGGGAAAAGACTACATTTATGCCGTAACACCGTTACTT GAAGATGCTTTAATGGATAGAGACCTTGTACACAGACAGACGGCTAGTGCAGTGGTACAGCACATGTCA CTTGGGGTTTATGGATTTGGTTGTGAAGATTCGCTGAATCACTTGTTGAACTATGTATGGCCCAATGTA TTTGAGACATCTCCTCATGTAATTCAGGCAGTTATGGGAGCCCTAGAGGGCCTGAGAGTTGCTATTGGA CCATGTAGAATGTTGCAATATTGTTTACAGGGTCTGTTTCACCCAGCCCGGAAAGTCAGAGATGTATAT TGGAAAATTTACAACTCCATCTACATTGGTTCCCAGGACGCTCTCATAGCACATTACCCAAGAATCTAC AACGATGATAAGAACACCTATATTCGTTATGAACTTGACTATATCTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC Protein Sequence (showhide) >Peptide sequence encoded by RC219605 Blue=ORF Red=Cloning site Green=Tag(s) MAKIAKTHEDIEAQIREIQGKKAALDEAQGVGLDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDD YSSSTSLLGQKKPGYHAPVALLNDIPQSTEQYDPFAEHRPPKIADREDEYKKHRRTMIISPERLDPFAD GGKTPDPKMNARTYMDVMREQHLTKEEREIRQQLAEKAKAGELKVVNGAAASQPPSKRKRRWDQTADQT PGATPKKLSSWDQAETPGHTPSLRWDETPGRAKGSETPGATPGSKIWDPTPSHTPAGAATPGRGDTPGH ATPGHGGATSSARKNRWDETPKTERDTPGHGSGWAETPRTDRGGDSIGETPTPGASKRKSRWDETPASQ MGGSTPVLTPGKTPIGTPAMNMATPTPGHIMSMTPEQLQAWRWEREIDERNRPLSDEELDAMFPEGYKV LPPPAGYVPIRTPARKLTATPTPLGGMTGFHMQTEDRTMKSVNDQPSGNLPFLKPDDIQYFDKLLVDVD ESTLSPEEQKERKIMKLLLKIKNGTPPMRKAALRQITDKAREFGAGPLFNQILPLLMSPTLEDQERHLL VKVIDRILYKLDDLVRPYVHKILVVIEPLLIDEDYYARVEGREIISNLAKAAGLATMISTMRPDIDNMD EYVRNTTARAFAVVASALGIPSLLPFLKAVCKSKKSWQARHTGIKIVQQIAILMGCAILPHLRSLVEII EHGLVDEQQKVRTISALAIAALAEAATPYGIESFDSVLKPLWKGIRQHRGKGLAAFLKAIGYLIPLMDA EYANYYTREVMLILIREFQSPDEEMKKIVLKVVKQCCGTDGVEANYIKTEILPPFFKHFWQHRMALDRR NYRQLVDTTVELANKVGAAEIISRIVDDLKDEAEQYRKMVMETIEKIMGNLGAADIDHKLEEQLIDGIL YAFQEQTTEDSVMLNGFGTVVNALGKRVKPYLPQICGTVLWRLNNKSAKVRQQAADLISRTAVVMKTCQ EEKLMGHLGVVLYEYLGEEYPEVLGSILGALKAIVNVIGMHKMTPPIKDLLPRLTPILKNRHEKVQENC IDLVGRIADRGAEYVSAREWMRICFELLELLKAHKKAIRRATVNTFGYIAKAIGPHDVLATLLNNLKVQ ERQNRVCTTVAIAIVAETCSPFTVLPALMNEYRVPELNVQNGVLKSLSFLFEYIGEMGKDYIYAVTPLL EDALMDRDLVHRQTASAVVQHMSLGVYGFGCEDSLNHLLNYVWPNVFETSPHVIQAVMGALEGLRVAIG PCRMLQYCLQGLFHPARKVRDVYWKIYNSIYIGSQDALIAHYPRIYNDDKNTYIRYELDYIL TRTRPLEQKLISEEDLAANDILDYKDDDDKV Recombinant protein using RC219605 also available, TP319605
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_012433
ORF Size	3912 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_012433.4
RefSeq Size	4338 bp
RefSeq ORF	3915 bp
Locus ID	23451
UniProt ID	O75533
Protein Pathways	Spliceosome
MW	145.8 kDa
Gene Summary	This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

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