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NDRG2 (NM_201535) Human Tagged ORF Clone
CAT＃: RC219812
NDRG2 (Myc-DDK-tagged)-Human NDRG family member 2 (NDRG2), transcript variant 1
ORF Plasmid: tGFP
"NM_201535" in other vectors (4)
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CNY 5,488.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SYLD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219812 representing NM_201535
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCTGCAGGAGGTGCAGATCACAGAGGAGAAGCCACTGTTGCCAGGACAGACGCCTGAGGCGG CCAAGGAGGCTGAGTTAGCTGCCCGAATCCTCCTGGACCAGGGACAGACTCACTCTGTGGAGACACCATA CGGCTCTGTCACTTTCACTGTCTATGGCACCCCCAAACCCAAACGCCCAGCGATCCTTACCTACCACGAT GTGGGACTCAACTATAAATCTTGCTTCCAGCCACTGTTTCAGTTCGAGGACATGCAGGAAATCATTCAGA ACTTTGTGCGGGTTCATGTGGATGCCCCTGGAATGGAAGAGGGAGCCCCTGTGTTCCCTTTGGGATATCA GTACCCATCTCTGGACGAGCTTGCAGACATGATCCCTTGCGTCCTGCAGTACCTAAATTTCTCTACAATA ATTGGAGTTGGTGTTGGAGCTGGAGCCTACATCCTGGCGAGATATGCTCTTAACCACCCGGACACTGTTG AAGGTCTTGTCCTCATCAACATTGATCCCAATGCCAAGGGTTGGATGGATTGGGCAGCCCACAAGCTAAC AGGCCTCACCTCTTCCATTCCGGAGATGATCCTTGGACATCTTTTCAGCCAGGAAGAGCTCTCTGGAAAT TCTGAGTTGATACAAAAGTACAGAAATATCATTACACATGCACCCAACCTGGATAACATTGAATTGTACT GGAACAGCTACAACAACCGCCGAGACCTGAACTTTGAGCGTGGAGGTGATATCACCCTCAGGTGTCCTGT GATGCTGGTGGTAGGAGACCAAGCACCTCATGAAGATGCAGTGGTGGAATGTAACTCAAAACTGGACCCC ACCCGGACCTCGTTCCTCAAGATGGCTGACTCCGGAGGTCAGCCCCAGCTGACTCAGCCAGGCAAGCTGA CCGAGGCCTTCAAGTACTTCCTGCAAGGCATGGGCTACATGGCCTCATCCTGCATGACTCGCCTGTCCCG GTCTCGTACAGCCTCTCTGACCAGTGCAGCATCCGTTGATGGCAACAGGTCCCGCTCTCGCACCCTGTCC CAGAGCAGCGAGTCTGGAACTCTTTCTTCGGGGCCCCCGGGGCACACCATGGAGGTCTCCTGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219812 representing NM_201535
Red=Cloning site Green=Tags(s) MAELQEVQITEEKPLLPGQTPEAAKEAELAARILLDQGQTHSVETPYGSVTFTVYGTPKPKRPAILTYHD VGLNYKSCFQPLFQFEDMQEIIQNFVRVHVDAPGMEEGAPVFPLGYQYPSLDELADMIPCVLQYLNFSTI IGVGVGAGAYILARYALNHPDTVEGLVLINIDPNAKGWMDWAAHKLTGLTSSIPEMILGHLFSQEELSGN SELIQKYRNIITHAPNLDNIELYWNSYNNRRDLNFERGGDITLRCPVMLVVGDQAPHEDAVVECNSKLDP TRTSFLKMADSGGQPQLTQPGKLTEAFKYFLQGMGYMASSCMTRLSRSRTASLTSAASVDGNRSRSRTLS QSSESGTLSSGPPGHTMEVSC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_201535 |
| ORF Size | 1113 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_201535.1, NP_963293.1 |
| RefSeq Size | 2142 bp |
| RefSeq ORF | 1116 bp |
| Locus ID | 57447 |
| UniProt ID | Q9UN36 |
| MW | 40.6 kDa |
| Gene Summary | This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may play a role in neurite outgrowth. This gene may be involved in glioblastoma carcinogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2017] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
MiR-483-5p and miR-139-5p promote aggressiveness by targeting N-myc downstream-regulated gene family members in adrenocortical cancer
,Agosta, C;Laugier, J;Guyon, L;Denis, J;Bertherat, J;Libé, R;Boisson, B;Sturm, N;Feige, JJ;Chabre, O;Cherradi, N;,
Int. J. Cancer
,PubMed ID 29516499
[NDRG2]
|
|
Epigenetic silencing of NDRG2 promotes colorectal cancer proliferation and invasion
,Hong, SN;Kim, SJ;Kim, ER;Chang, DK;Kim, YH;,
J. Gastroenterol. Hepatol.
,PubMed ID 26250123
[NDRG2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219812L3 | Lenti-ORF clone of NDRG2 (Myc-DDK-tagged)-Human NDRG family member 2 (NDRG2), transcript variant 1 |
CNY 7,888.00 |
|
| RC219812L4 | Lenti-ORF clone of NDRG2 (mGFP-tagged)-Human NDRG family member 2 (NDRG2), transcript variant 1 |
CNY 5,890.00 |
|
| RG219812 | NDRG2 (tGFP-tagged) - Human NDRG family member 2 (NDRG2), transcript variant 1 |
CNY 7,088.00 |
|
| SC310475 | NDRG2 (untagged)-Human NDRG family member 2 (NDRG2), transcript variant 1 |
CNY 5,488.00 |
