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PAX3 (NM_181459) Human Tagged ORF Clone
CAT＃: RC219920
PAX3 (Myc-DDK-tagged)-Human paired box 3 (PAX3), transcript variant PAX3E
ORF Plasmid: tGFP
"NM_181459" in other vectors (4)
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CNY 5,640.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CDHS; HUP2; WS1; WS3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219920 representing NM_181459
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCACGCTGGCCGGCGCTGTGCCCAGGATGATGCGGCCGGGCCCGGGGCAGAACTACCCGCGTAGCG GGTTCCCGCTGGAAGTGTCCACTCCCCTCGGCCAGGGCCGCGTCAACCAGCTCGGCGGTGTTTTTATCAA CGGCAGGCCGCTGCCCAACCACATCCGCCACAAGATCGTGGAGATGGCCCACCACGGCATCCGGCCCTGC GTCATCTCGCGCCAGCTGCGCGTGTCCCACGGCTGCGTCTCCAAGATCCTGTGCAGGTACCAGGAGACTG GCTCCATACGTCCTGGTGCCATCGGCGGCAGCAAGCCCAAGCAGGTGACAACGCCTGACGTGGAGAAGAA AATTGAGGAATACAAAAGAGAGAACCCGGGCATGTTCAGCTGGGAAATCCGAGACAAATTACTCAAGGAC GCGGTCTGTGATCGAAACACCGTGCCGTCAGTGAGTTCCATCAGCCGCATCCTGAGAAGTAAATTCGGGA AAGGTGAAGAGGAGGAGGCCGACTTGGAGAGGAAGGAGGCAGAGGAAAGCGAGAAGAAGGCCAAACACAG CATCGACGGCATCCTGAGCGAGCGAGCCTCAGCACCCCAATCAGATGAAGGCTCTGATATTGACTCTGAA CCAGATTTACCACTAAAGAGGAAACAGCGCAGAAGCCGAACCACCTTCACAGCAGAACAGCTGGAGGAAC TGGAGCGTGCTTTTGAGAGAACTCATTACCCTGACATTTATACTAGGGAGGAACTGGCCCAGAGGGCGAA GCTCACCGAGGCCCGAGTACAGGTCTGGTTTAGCAACCGCCGTGCAAGATGGAGGAAGCAAGCTGGGGCC AATCAACTGATGGCTTTCAACCATCTCATTCCCGGGGGGTTCCCTCCCACTGCCATGCCGACCTTGCCAA CGTACCAGCTGTCGGAGACCTCTTACCAGCCCACATCTATTCCACAAGCTGTGTCAGATCCCAGCAGCAC CGTTCACAGACCTCAACCGCTTCCTCCAAGCACTGTACACCAAAGCACGATTCCTTCCAACCCAGACAGC AGCTCTGCCTACTGCCTCCCCAGCACCAGGCATGGATTTTCCAGCTATACAGACAGCTTTGTGCCTCCGT CGGGGCCCTCCAACCCCATGAACCCCACCATTGGCAATGGCCTCTCACCTCAGGTAATGGGACTCCTGAC CAACCACGGTGGGGTACCTCATCAGCCCCAGACTGATTACGCGCTCTCCCCTCTCACCGGGGGTCTGGAA CCTACCACCACGGTGTCGGCCAGCTGCAGTCAGAGACTAGACCATATGAAGAGCTTGGACAGTCTGCCAA CATCTCAGTCCTACTGTCCACCCACCTATAGCACCACAGGCTACAGTATGGACCCTGTCACAGGCTACCA ATATGGGCAGTATGGACAAAGTGCCTTTCATTATCTCAAGCCAGATATCGCTTGGTTTCAAATCCTTTTG AACACGTTCGACAAAAGCAGTGGAGAAGAGGAAGACCTGGAGCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219920 representing NM_181459
Red=Cloning site Green=Tags(s) MTTLAGAVPRMMRPGPGQNYPRSGFPLEVSTPLGQGRVNQLGGVFINGRPLPNHIRHKIVEMAHHGIRPC VISRQLRVSHGCVSKILCRYQETGSIRPGAIGGSKPKQVTTPDVEKKIEEYKRENPGMFSWEIRDKLLKD AVCDRNTVPSVSSISRILRSKFGKGEEEEADLERKEAEESEKKAKHSIDGILSERASAPQSDEGSDIDSE PDLPLKRKQRRSRTTFTAEQLEELERAFERTHYPDIYTREELAQRAKLTEARVQVWFSNRRARWRKQAGA NQLMAFNHLIPGGFPPTAMPTLPTYQLSETSYQPTSIPQAVSDPSSTVHRPQPLPPSTVHQSTIPSNPDS SSAYCLPSTRHGFSSYTDSFVPPSGPSNPMNPTIGNGLSPQVMGLLTNHGGVPHQPQTDYALSPLTGGLE PTTTVSASCSQRLDHMKSLDSLPTSQSYCPPTYSTTGYSMDPVTGYQYGQYGQSAFHYLKPDIAWFQILL NTFDKSSGEEEDLEQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_181459 |
| ORF Size | 1515 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_181459.4 |
| RefSeq Size | 3170 bp |
| RefSeq ORF | 1518 bp |
| Locus ID | 5077 |
| UniProt ID | P23760 |
| Protein Families | Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors |
| MW | 55.8 kDa |
| Gene Summary | This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219920L3 | Lenti ORF clone of Human paired box 3 (PAX3), transcript variant PAX3E, Myc-DDK-tagged |
CNY 6,270.00 |
|
| RC219920L4 | Lenti ORF clone of Human paired box 3 (PAX3), transcript variant PAX3E, mGFP tagged |
CNY 8,040.00 |
|
| RG219920 | PAX3 (tGFP-tagged) - Human paired box 3 (PAX3), transcript variant PAX3E |
CNY 7,240.00 |
|
| SC309287 | PAX3 (untagged)-Human paired box 3 (PAX3), transcript variant PAX3E |
CNY 8,080.00 |
