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Neurotrypsin (PRSS12) (NM_003619) Human Tagged ORF Clone
CAT＃: RC220241
PRSS12 (Myc-DDK-tagged)-Human protease, serine, 12 (neurotrypsin, motopsin) (PRSS12)
ORF Plasmid: tGFP
"NM_003619" in other vectors (6)
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CNY 9,608.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BSSP-3; BSSP3; MRT1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220241 representing NM_003619
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGCTCGCCCGCTTCGTGCTAGCCCTGATGTTAGGGGCGCTCCCCGAAGTGGTCGGCTTTGATTCTG TCCTCAATGATTCCCTCCACCACAGCCACCGCCATTCGCCCCCTGCGGGTCCGCACTACCCCTATTACCT TCCCACCCAGCAGCGGCCCCCGAGGACGCGTCCGCCGCCGCCTCTCCCGCGCTTCCCGCGCCCCCCGCGG GCGCTCCCTGCCCAGCGCCCGCACGCCCTCCAGGCCGGGCACACGCCCCGGCCGCACCCCTGGGGCTGCC CCGCCGGCGAGCCATGGGTCAGCGTGACGGACTTCGGCGCCCCGTGTCTGCGGTGGGCGGAGGTGCCACC CTTCCTGGAGCGGTCGCCCCCAGCGAGCTGGGCTCAGCTGCGAGGACAGCGCCACAACTTTTGTCGGAGC CCCGACGGCGCGGGCAGACCCTGGTGTTTCTACGGAGACGCCCGTGGCAAGGTGGACTGGGGCTACTGCG ACTGCAGACACGGATCAGTACGACTTCGTGGCGGCAAAAATGAGTTTGAAGGCACAGTGGAAGTATATGC AAGTGGAGTTTGGGGCACTGTCTGTAGCAGCCACTGGGATGATTCTGATGCATCAGTCATTTGTCACCAG CTGCAGCTGGGAGGAAAAGGAATAGCAAAACAAACCCCGTTTTCTGGACTGGGCCTTATTCCCATTTATT GGAGCAATGTCCGTTGCCGAGGAGATGAAGAAAATATACTGCTTTGTGAAAAAGACATCTGGCAGGGTGG GGTGTGTCCTCAGAAGATGGCAGCTGCTGTCACGTGTAGCTTTTCCCATGGCCCAACGTTCCCCATCATT CGCCTTGCTGGAGGCAGCAGTGTGCATGAAGGCCGGGTGGAGCTCTACCATGCTGGCCAGTGGGGAACCG TTTGTGATGACCAATGGGATGATGCCGATGCAGAAGTGATCTGCAGGCAGCTGGGCCTCAGTGGCATTGC CAAAGCATGGCATCAGGCATATTTTGGGGAAGGGTCTGGCCCAGTTATGTTGGATGAAGTACGCTGCACT GGGAATGAGCTTTCAATTGAGCAGTGTCCAAAGAGCTCCTGGGGAGAGCATAACTGTGGCCATAAAGAAG ATGCTGGAGTGTCCTGTACCCCTCTAACAGATGGGGTCATCAGACTTGCAGGTGGGAAAGGCAGCCATGA GGGTCGCTTGGAGGTATATTACAGAGGCCAGTGGGGAACTGTCTGTGATGATGGCTGGACTGAGCTGAAT ACATACGTGGTTTGTCGACAATTGGGATTTAAATATGGTAAACAAGCATCTGCCAACCATTTTGAAGAAA GCACAGGGCCCATATGGTTGGATGACGTCAGCTGCTCAGGAAAGGAAACCAGATTTCTTCAGTGTTCCAG GCGACAGTGGGGAAGGCATGACTGCAGCCACCGCGAAGATGTTAGCATTGCCTGCTACCCTGGCGGCGAG GGACACAGGCTCTCTCTGGGTTTTCCTGTCAGACTGATGGATGGAGAAAATAAGAAAGAAGGACGAGTGG AGGTTTTTATCAATGGCCAGTGGGGAACAATCTGTGATGATGGATGGACTGATAAGGATGCAGCTGTGAT CTGTCGTCAGCTTGGCTACAAGGGTCCTGCCAGAGCAAGAACCATGGCTTACTTTGGAGAAGGAAAAGGA CCCATCCATGTGGATAATGTGAAGTGCACAGGAAATGAGAGGTCCTTGGCTGACTGTATCAAGCAAGATA TTGGAAGACACAACTGCCGCCACAGTGAAGATGCAGGAGTTATTTGTGATTATTTTGGCAAGAAGGCCTC AGGTAACAGTAATAAAGAGTCCCTCTCATCTGTTTGTGGCTTGAGATTACTGCACCGTCGGCAGAAGCGG ATCATTGGTGGGAAAAATTCTTTAAGGGGTGGTTGGCCTTGGCAGGTTTCCCTCCGGCTGAAGTCATCCC ATGGAGATGGCAGGCTCCTCTGCGGGGCTACGCTCCTGAGTAGCTGCTGGGTCCTCACAGCAGCACACTG TTTCAAGAGGTATGGCAACAGCACTAGGAGCTATGCTGTTAGGGTTGGAGATTATCATACTCTGGTACCA GAGGAGTTTGAGGAAGAAATTGGAGTTCAACAGATTGTGATTCATCGGGAGTATCGACCCGACCGCAGTG ATTATGACATAGCCCTGGTTAGATTACAAGGACCAGAAGAGCAATGTGCCAGATTCAGCAGCCATGTTTT GCCAGCCTGTTTACCACTCTGGAGAGAGAGGCCACAGAAAACAGCATCCAACTGTTACATAACAGGATGG GGTGACACAGGACGAGCCTATTCAAGAACACTACAACAAGCAGCCATTCCCTTACTTCCTAAAAGGTTTT GTGAAGAACGTTATAAGGGTCGGTTTACAGGGAGAATGCTTTGTGCTGGAAACCTCCATGAACACAAACG CGTGGACAGCTGCCAGGGAGACAGCGGAGGACCACTCATGTGTGAACGGCCCGGAGAGAGCTGGGTGGTG TATGGGGTGACCTCCTGGGGGTATGGCTGTGGAGTCAAGGATTCTCCTGGTGTTTATACCAAAGTCTCAG CCTTTGTACCTTGGATAAAAAGTGTCACCAAACTG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC220241 representing NM_003619
Red=Cloning site Green=Tags(s) MTLARFVLALMLGALPEVVGFDSVLNDSLHHSHRHSPPAGPHYPYYLPTQQRPPRTRPPPPLPRFPRPPR ALPAQRPHALQAGHTPRPHPWGCPAGEPWVSVTDFGAPCLRWAEVPPFLERSPPASWAQLRGQRHNFCRS PDGAGRPWCFYGDARGKVDWGYCDCRHGSVRLRGGKNEFEGTVEVYASGVWGTVCSSHWDDSDASVICHQ LQLGGKGIAKQTPFSGLGLIPIYWSNVRCRGDEENILLCEKDIWQGGVCPQKMAAAVTCSFSHGPTFPII RLAGGSSVHEGRVELYHAGQWGTVCDDQWDDADAEVICRQLGLSGIAKAWHQAYFGEGSGPVMLDEVRCT GNELSIEQCPKSSWGEHNCGHKEDAGVSCTPLTDGVIRLAGGKGSHEGRLEVYYRGQWGTVCDDGWTELN TYVVCRQLGFKYGKQASANHFEESTGPIWLDDVSCSGKETRFLQCSRRQWGRHDCSHREDVSIACYPGGE GHRLSLGFPVRLMDGENKKEGRVEVFINGQWGTICDDGWTDKDAAVICRQLGYKGPARARTMAYFGEGKG PIHVDNVKCTGNERSLADCIKQDIGRHNCRHSEDAGVICDYFGKKASGNSNKESLSSVCGLRLLHRRQKR IIGGKNSLRGGWPWQVSLRLKSSHGDGRLLCGATLLSSCWVLTAAHCFKRYGNSTRSYAVRVGDYHTLVP EEFEEEIGVQQIVIHREYRPDRSDYDIALVRLQGPEEQCARFSSHVLPACLPLWRERPQKTASNCYITGW GDTGRAYSRTLQQAAIPLLPKRFCEERYKGRFTGRMLCAGNLHEHKRVDSCQGDSGGPLMCERPGESWVV YGVTSWGYGCGVKDSPGVYTKVSAFVPWIKSVTKL SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003619 |
| ORF Size | 2625 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003619.4 |
| RefSeq Size | 4573 bp |
| RefSeq ORF | 2628 bp |
| Locus ID | 8492 |
| UniProt ID | P56730 |
| Domains | KR, SR, Tryp_SPc |
| Protein Families | Druggable Genome, Protease, Secreted Protein |
| MW | 97.1 kDa |
| Gene Summary | This gene encodes a member of the trypsin family of serine proteases and contains a signal peptide, a proline-rich region, a Kringle domain, four scavenger receptor cysteine-rich domains, and a trypsin-like serine protease domain. The protein, sometimes referred to as neurotrypsin or motopsin, is secreted from neuronal cells and localizes to the synaptic cleft. Studies in mice show that this protein cleaves a protein, agrin, that is important for the formation and maintenance of exitatory synapses. Defects in this gene cause a form of autosomal recessive cognitive impairment (MRT1). [provided by RefSeq, Jul 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220241L1 | Lenti-ORF clone of PRSS12 (Myc-DDK-tagged)-Human protease, serine, 12 (neurotrypsin, motopsin) (PRSS12) |
CNY 12,008.00 |
|
| RC220241L2 | Lenti-ORF clone of PRSS12 (mGFP-tagged)-Human protease, serine, 12 (neurotrypsin, motopsin) (PRSS12) |
CNY 11,120.00 |
|
| RC220241L3 | Lenti-ORF clone of PRSS12 (Myc-DDK-tagged)-Human protease, serine, 12 (neurotrypsin, motopsin) (PRSS12) |
CNY 11,120.00 |
|
| RC220241L4 | Lenti-ORF clone of PRSS12 (mGFP-tagged)-Human protease, serine, 12 (neurotrypsin, motopsin) (PRSS12) |
CNY 11,120.00 |
|
| RG220241 | PRSS12 (tGFP-tagged) - Human protease, serine, 12 (neurotrypsin, motopsin) (PRSS12) |
CNY 10,170.00 |
|
| SC126501 | PRSS12 (untagged)-Human protease, serine, 12 (neurotrypsin, motopsin) (PRSS12) |
CNY 9,624.00 |
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