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Contactin 1 (CNTN1) (NM_175038) Human Tagged ORF Clone
CAT＃: RC220493
CNTN1 (Myc-DDK-tagged)-Human contactin 1 (CNTN1), transcript variant 2
ORF Plasmid: tGFP
"NM_175038" in other vectors (4)
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CNY 7,016.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | F3; GP135; MYPCN |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220493 representing NM_175038
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAATGTGGTTGCTGGTCAGTCATCTTGTGATAATATCTATTACTACCTGTTTAGCAGTTTCTGAGG AAGACAAAGGATTTGGACCAATTTTTGAAGAGCAGCCAATCAATACCATTTATCCAGAGGAATCACTGGA AGGAAAAGTCTCACTCAACTGTAGGGCACGAGCCAGCCCTTTCCCGGTTTACAAATGGAGAATGAATAAT GGGGACGTTGATCTCACAAGTGATCGATACAGTATGGTAGGAGGAAACCTTGTTATCAACAACCCTGACA AACAGAAAGATGCTGGAATATACTACTGTTTAGCATCTAATAACTACGGGATGGTCAGAAGCACTGAAGC AACCCTGAGCTTTGGATATCTTGATCCTTTCCCACCTGAGGAACGTCCTGAGGTCAGAGTAAAAGAAGGG AAAGGAATGGTGCTTCTCTGTGACCCCCCATACCATTTTCCAGATGATCTTAGCTATCGCTGGCTTCTAA ATGAATTTCCTGTATTTATCACAATGGATAAACGGCGATTTGTGTCTCAGACAAATGGCAATCTCTACAT TGCAAATGTTGAGGCTTCCGACAAAGGCAATTATTCCTGCTTTGTTTCCAGTCCTTCTATTACAAAGAGC GTGTTCAGCAAATTCATCCCACTCATTCCAATACCTGAACGAACAACAAAACCATATCCTGCTGATATTG TAGTTCAGTTCAAGGATGTATATGCATTGATGGGCCAAAATGTGACCTTAGAATGTTTTGCACTTGGAAA TCCTGTTCCGGATATCCGATGGCGGAAGGTTCTAGAACCAATGCCAAGCACTGCTGAGATTAGCACCTCT GGGGCTGTTCTTAAGATCTTCAATATTCAGCTAGAAGATGAAGGCATCTATGAATGTGAGGCTGAGAACA TTAGAGGAAAGGATAAACATCAAGCAAGAATTTATGTTCAAGCATTCCCTGAGTGGGTAGAACACATCAA TGACACAGAGGTGGACATAGGCAGTGATCTCTACTGGCCTTGTGTGGCCACAGGAAAGCCCATCCCTACA ATCCGATGGTTGAAAAATGGATATGCGTATCATAAAGGGGAATTAAGACTGTATGATGTGACTTTTGAAA ATGCCGGAATGTATCAGTGCATAGCTGAAAACACATATGGAGCCATTTATGCAAATGCTGAGTTGAAGAT CTTGGCGTTGGCTCCAACTTTTGAAATGAATCCTATGAAGAAAAAGATCCTGGCTGCTAAAGGTGGAAGG GTGATAATTGAATGCAAACCTAAAGCTGCACCGAAACCAAAGTTTTCATGGAGTAAAGGGACAGAGTGGC TTGTCAATAGCAGCAGAATACTCATTTGGGAAGATGGTAGCTTGGAAATCAACAACATTACAAGGAATGA TGGAGGTATCTATACATGCTTTGCAGAAAATAACAGAGGGAAAGCTAATAGCACTGGAACCCTTGTTATC ACAGATCCTACGCGAATTATATTGGCCCCAATTAATGCCGATATCACAGTTGGAGAAAACGCCACCATGC AGTGTGCTGCGTCCTTTGATCCTGCCTTGGATCTCACATTTGTTTGGTCCTTCAATGGCTATGTGATCGA TTTTAACAAAGAGAATATTCACTACCAGAGGAATTTTATGCTGGATTCCAATGGGGAATTACTAATCCGA AATGCGCAGCTGAAACATGCTGGAAGATACACATGCACTGCCCAGACAATTGTGGACAATTCTTCAGCTT CAGCTGACCTTGTAGTGAGAGGCCCTCCAGGCCCTCCAGGTGGTCTGAGAATAGAAGACATTAGAGCCAC TTCTGTGGCACTTACTTGGAGCCGTGGTTCAGACAATCATAGTCCTATTTCTAAATACACTATCCAGACC AAGACTATTCTTTCAGATGACTGGAAAGATGCAAAGACAGATCCCCCAATTATTGAAGGAAATATGGAGG CAGCAAGAGCAGTGGACTTAATCCCATGGATGGAGTATGAATTCCGCGTGGTAGCAACCAATACACTGGG TAGAGGAGAGCCCAGTATACCATCTAACAGAATTAAAACAGACGGTGCTGCACCAAATGTGGCTCCTTCA GATGTAGGAGGTGGAGGTGGAAGAAACAGAGAGCTGACCATAACATGGGCGCCTTTGTCAAGAGAATACC ACTATGGCAACAATTTTGGTTACATAGTGGCATTTAAGCCATTTGATGGAGAAGAATGGAAAAAAGTCAC AGTTACTAATCCTGATACTGGCCGATATGTCCATAAAGATGAAACCATGAGCCCTTCCACTGCATTTCAA GTTAAAGTCAAGGCCTTCAACAACAAAGGAGATGGACCTTACAGCCTAGTAGCAGTCATTAATTCAGCAC AAGACGCTCCCAGTGAAGCCCCAACAGAAGTAGGTGTAAAAGTCTTATCATCTTCTGAGATATCTGTTCA TTGGGAACATGTTTTAGAAAAAATAGTGGAAAGCTATCAGATTCGGTATTGGGCTGCCCATGACAAAGAA GAAGCTGCAAACAGAGTTCAAGTCACCAGCCAAGAGTACTCGGCCAGGCTCGAGAACCTTCTGCCAGACA CCCAGTATTTTATAGAAGTCGGGGCCTGCAATAGTGCAGGGTGTGGACCTCCAAGTGACATGATTGAGGC TTTCACCAAGAAAGCACCTCCTAGCCAGCCTCCAAGGATCATCAGTTCAGTAAGGTCTGGTTCACGCTAT ATAATCACCTGGGATCATGTCGTTGCACTATCAAATGAATCTACAGTGACGGGATATAAGGTACTCTACA GACCTGATGGCCAGCATGATGGCAAGCTGTATTCAACTCACAAACACTCCATAGAAGTCCCAATCCCCAG AGATGGAGAATACGTTGTGGAGGTTCGCGCGCACAGTGATGGAGGAGATGGAGTGGTGTCTCAAGTCAAA ATTTCAGGTGCACCCACCCTATCCCCAAGTCTTCTCGGCTTACTGCTGCCTGCCTTTGGCATCCTTGTCT ACTTGGAATTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220493 representing NM_175038
Red=Cloning site Green=Tags(s) MKMWLLVSHLVIISITTCLAVSEEDKGFGPIFEEQPINTIYPEESLEGKVSLNCRARASPFPVYKWRMNN GDVDLTSDRYSMVGGNLVINNPDKQKDAGIYYCLASNNYGMVRSTEATLSFGYLDPFPPEERPEVRVKEG KGMVLLCDPPYHFPDDLSYRWLLNEFPVFITMDKRRFVSQTNGNLYIANVEASDKGNYSCFVSSPSITKS VFSKFIPLIPIPERTTKPYPADIVVQFKDVYALMGQNVTLECFALGNPVPDIRWRKVLEPMPSTAEISTS GAVLKIFNIQLEDEGIYECEAENIRGKDKHQARIYVQAFPEWVEHINDTEVDIGSDLYWPCVATGKPIPT IRWLKNGYAYHKGELRLYDVTFENAGMYQCIAENTYGAIYANAELKILALAPTFEMNPMKKKILAAKGGR VIIECKPKAAPKPKFSWSKGTEWLVNSSRILIWEDGSLEINNITRNDGGIYTCFAENNRGKANSTGTLVI TDPTRIILAPINADITVGENATMQCAASFDPALDLTFVWSFNGYVIDFNKENIHYQRNFMLDSNGELLIR NAQLKHAGRYTCTAQTIVDNSSASADLVVRGPPGPPGGLRIEDIRATSVALTWSRGSDNHSPISKYTIQT KTILSDDWKDAKTDPPIIEGNMEAARAVDLIPWMEYEFRVVATNTLGRGEPSIPSNRIKTDGAAPNVAPS DVGGGGGRNRELTITWAPLSREYHYGNNFGYIVAFKPFDGEEWKKVTVTNPDTGRYVHKDETMSPSTAFQ VKVKAFNNKGDGPYSLVAVINSAQDAPSEAPTEVGVKVLSSSEISVHWEHVLEKIVESYQIRYWAAHDKE EAANRVQVTSQEYSARLENLLPDTQYFIEVGACNSAGCGPPSDMIEAFTKKAPPSQPPRIISSVRSGSRY IITWDHVVALSNESTVTGYKVLYRPDGQHDGKLYSTHKHSIEVPIPRDGEYVVEVRAHSDGGDGVVSQVK ISGAPTLSPSLLGLLLPAFGILVYLEF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175038 |
| ORF Size | 3021 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_175038.2, NP_778203.1 |
| RefSeq Size | 3394 bp |
| RefSeq ORF | 3024 bp |
| Locus ID | 1272 |
| UniProt ID | Q12860 |
| Protein Pathways | Cell adhesion molecules (CAMs) |
| MW | 109.6 kDa |
| Gene Summary | The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220493L3 | Lenti ORF clone of Human contactin 1 (CNTN1), transcript variant 2, Myc-DDK-tagged |
CNY 11,970.00 |
|
| RC220493L4 | Lenti ORF clone of Human contactin 1 (CNTN1), transcript variant 2, mGFP tagged |
CNY 11,970.00 |
|
| RG220493 | CNTN1 (tGFP-tagged) - Human contactin 1 (CNTN1), transcript variant 2 |
CNY 11,120.00 |
|
| SC310010 | CNTN1 (untagged)-Human contactin 1 (CNTN1), transcript variant 2 |
CNY 19,290.00 |
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