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SIDT2 (NM_001040455) Human Tagged ORF Clone
CAT＃: RC220528
- TrueORF®
SIDT2 (Myc-DDK-tagged)-Human SID1 transmembrane family, member 2 (SIDT2)
ORF Plasmid: tGFP
"NM_001040455" in other vectors (4)
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CNY 8,740.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CGI-40 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220528 representing NM_001040455
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCGCTCTGGGCTTGCCCTTCTTGGTGCTCTTGGTGGCCTCGGTCGAGAGCCATCTGGGGGTTCTGG GGCCCAAGAACGTCTCGCAGAAAGACGCCGAGTTTGAGCGCACCTACGTGGACGAGGTCAACAGCGAGCT GGTCAACATCTACACCTTCAACCATACTGTGACCCGCAACAGGACAGAGGGCGTGCGTGTGTCTGTGAAC GTCCTGAACAAGCAGAAGGGGGCGCCGTTGCTGTTTGTGGTCCGCCAGAAGGAGGCTGTGGTGTCCTTCC AGGTGCCCCTAATCCTGCGAGGGATGTTTCAGCGCAAGTACCTCTACCAAAAAGTGGAACGAACCCTGTG TCAGCCCCCCACCAAGAATGAGTCGGAGATTCAGTTCTTCTACGTGGATGTGTCCACCCTGTCACCAGTC AACACCACATACCAGCTCCGGGTCAGCCGCATGGACGATTTTGTGCTCAGGACTGGGGAGCAGTTCAGCT TCAATACCACAGCAGCACAGCCCCAGTACTTCAAGTATGAGTTCCCTGAAGGCGTGGACTCGGTAATTGT CAAGGTGACCTCCAACAAGGCCTTCCCCTGCTCAGTCATCTCCATTCAGGATGTGCTGTGTCCTGTCTAT GACCTGGACAACAACGTAGCCTTCATCGGCATGTACCAGACGATGACCAAGAAGGCGGCCATCACCGTAC AGCGCAAAGACTTCCCCAGCAACAGCTTTTATGTGGTGGTGGTGGTGAAGACCGAAGACCAAGCCTGCGG GGGCTCCCTGCCTTTCTACCCCTTCGCAGAAGATGAACCGGTCGATCAAGGGCACCGCCAGAAAACCCTG TCAGTGCTGGTGTCTCAAGCAGTCACGTCTGAGGCATACGTCAGTGGGATGCTCTTTTGCCTGGGTATAT TTCTCTCCTTTTACCTGCTGACCGTCCTCCTGGCCTGCTGGGAGAACTGGAGGCAGAAGAAGAAGACCCT GCTGGTGGCCATTGACCGAGCCTGCCCAGAAAGCGGTCACCCTCGAGTCCTGGCTGATTCTTTTCCTGGC AGTTCCCCTTATGAGGGTTACAACTATGGCTCCTTTGAGAATGTTTCTGGATCTACCGATGGTCTGGTTG ACAGCGCTGGCACTGGGGACCTCTCTTACGGTTACCAGGGCCGCTCCTTTGAACCTGTAGGTACTCGGCC CCGAGTGGACTCCATGAGCTCTGTGGAGGAGGATGACTACGACACATTGACCGACATCGATTCCGACAAG AATGTCATTCGCACCAAGCAATACCTCTATGTGGCTGACCTGGCACGGAAGGACAAGCGTGTTCTGCGGA AAAAGTACCAGATCTACTTCTGGAACATTGCCACCATTGCTGTCTTCTATGCCCTTCCTGTGGTGCAGCT GGTGATCACCTACCAGACGGTGGTGAATGTCACAGGGAATCAGGACATCTGCTACTACAACTTCCTCTGC GCCCACCCACTGGGCAATCTCAGCGCCTTCAACAACATCCTCAGCAACCTGGGGTACATCCTGCTGGGGC TGCTTTTCCTGCTCATCATCCTGCAACGGGAGATCAACCACAACCGGGCCCTGCTGCGCAATGACCTCTG TGCCCTGGAATGTGGGATCCCCAAACACTTTGGGCTTTTCTACGCCATGGGCACAGCCCTGATGATGGAG GGGCTGCTCAGTGCTTGCTATCATGTGTGCCCCAACTATACCAATTTCCAGTTTGACACATCGTTCATGT ACATGATCGCCGGACTCTGCATGCTGAAGCTCTACCAGAAGCGGCACCCGGACATCAACGCCAGCGCCTA CAGTGCCTACGCCTGCCTGGCCATTGTCATCTTCTTCTCTGTGCTGGGCGTGGTCTTTGGCAAAGGGAAC ACGGCGTTCTGGATCGTCTTCTCCATCATTCACATCATCGCCACCCTGCTCCTCAGCACGCAGCTCTATT ACATGGGCCGGTGGAAACTGGACTCGGGGATCTTCCGCCGCATCCTCCACGTGCTCTACACAGACTGCAT CCGGCAGTGCAGCGGGCCGCTCTACGTGGACCGCATGGTGCTGCTGGTCATGGGCAACGTCATCAACTGG TCGCTGGCTGCCTATGGGCTTATCATGCGCCCCAATGATTTCGCTTCCTACTTGTTGGCCATTGGCATCT GCAACCTGCTCCTTTACTTCGCCTTCTACATCATCATGAAGCTCCGGAGTGGGGAGAGGATCAAGCTCAT CCCCCTGCTCTGCATCGTTTGCACCTCCGTGGTCTGGGGCTTCGCGCTCTTCTTCTTCTTCCAGGGACTC AGCACCTGGCAGAAAACCCCTGCAGAGTCGAGGGAGCACAACCGGGACTGCATCCTCCTCGACTTCTTTG ACGACCACGACATCTGGCACTTCCTCTCCTCCATCGCCATGTTCGGGTCCTTCCTGGTGTTGCTGACACT GGATGACGACCTGGATACTGTGCAGCGGGACAAGATCTATGTCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220528 representing NM_001040455
Red=Cloning site Green=Tags(s) MFALGLPFLVLLVASVESHLGVLGPKNVSQKDAEFERTYVDEVNSELVNIYTFNHTVTRNRTEGVRVSVN VLNKQKGAPLLFVVRQKEAVVSFQVPLILRGMFQRKYLYQKVERTLCQPPTKNESEIQFFYVDVSTLSPV NTTYQLRVSRMDDFVLRTGEQFSFNTTAAQPQYFKYEFPEGVDSVIVKVTSNKAFPCSVISIQDVLCPVY DLDNNVAFIGMYQTMTKKAAITVQRKDFPSNSFYVVVVVKTEDQACGGSLPFYPFAEDEPVDQGHRQKTL SVLVSQAVTSEAYVSGMLFCLGIFLSFYLLTVLLACWENWRQKKKTLLVAIDRACPESGHPRVLADSFPG SSPYEGYNYGSFENVSGSTDGLVDSAGTGDLSYGYQGRSFEPVGTRPRVDSMSSVEEDDYDTLTDIDSDK NVIRTKQYLYVADLARKDKRVLRKKYQIYFWNIATIAVFYALPVVQLVITYQTVVNVTGNQDICYYNFLC AHPLGNLSAFNNILSNLGYILLGLLFLLIILQREINHNRALLRNDLCALECGIPKHFGLFYAMGTALMME GLLSACYHVCPNYTNFQFDTSFMYMIAGLCMLKLYQKRHPDINASAYSAYACLAIVIFFSVLGVVFGKGN TAFWIVFSIIHIIATLLLSTQLYYMGRWKLDSGIFRRILHVLYTDCIRQCSGPLYVDRMVLLVMGNVINW SLAAYGLIMRPNDFASYLLAIGICNLLLYFAFYIIMKLRSGERIKLIPLLCIVCTSVVWGFALFFFFQGL STWQKTPAESREHNRDCILLDFFDDHDIWHFLSSIAMFGSFLVLLTLDDDLDTVQRDKIYVF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001040455 |
| ORF Size | 2496 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001040455.2 |
| RefSeq Size | 3919 bp |
| RefSeq ORF | 2499 bp |
| Locus ID | 51092 |
| UniProt ID | Q8NBJ9 |
| Protein Families | Transmembrane |
| MW | 94.3 kDa |
| Gene Summary | Mediates the translocation of RNA and DNA across the lysosomal membrane during RNA and DNA autophagy (RDA), a process in which RNA or DNA is directly imported into lysosomes in an ATP-dependent manner, and degraded (PubMed:27046251, PubMed:27846365). Involved in the uptake of single-stranded oligonucleotides by living cells, a process called gymnosis (PubMed:28277980). Involved in the uptake of single-stranded oligonucleotides by living cells, a process called gymnosis. In vitro, mediates the uptake of linear DNA more efficiently than that of circular DNA, but exhibits similar uptake efficacy toward RNA and DNA. Binds long double-stranded RNA (dsRNA) (500 - 700 base pairs), but not dsRNA shorter than 100 bp (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A C. elegans model of human a1-antitrypsin deficiency links components of the RNAi pathway to misfolded protein turnover
,Long, OS;Benson, JA;Kwak, JH;Luke, CJ;Gosai, SJ;O'Reilly, LP;Wang, Y;Li, J;Vetica, AC;Miedel, MT;Stolz, DB;Watkins, SC;Züchner, S;Perlmutter, DH;Silverman, GA;Pak, SC;,
Hum. Mol. Genet.
,PubMed ID 24838286
[SIDT2]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220528L3 | Lenti-ORF clone of SIDT2 (Myc-DDK-tagged)-Human SID1 transmembrane family, member 2 (SIDT2) |
CNY 8,496.00 |
|
| RC220528L4 | Lenti-ORF clone of SIDT2 (mGFP-tagged)-Human SID1 transmembrane family, member 2 (SIDT2) |
CNY 10,640.00 |
|
| RG220528 | SIDT2 (tGFP-tagged) - Human SID1 transmembrane family, member 2 (SIDT2) |
CNY 7,696.00 |
|
| SC316840 | SIDT2 (untagged)-Human SID1 transmembrane family, member 2 (SIDT2) |
CNY 6,104.00 |
