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DGAT1 (NM_012079) Human Tagged ORF Clone
CAT＃: RC220595
- TrueORF®
DGAT1 (Myc-DDK-tagged)-Human diacylglycerol O-acyltransferase 1 (DGAT1)
ORF Plasmid: tGFP
"NM_012079" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARAT; ARGP1; DGAT; DIAR7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220595 representing NM_012079
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGACCGCGGCAGCTCCCGGCGCCGGAGGACAGGGTCGCGGCCCTCGAGCCACGGCGGCGGCGGGC CTGCGGCGGCGGAAGAGGAGGTGCGGGACGCCGCTGCGGGCCCCGACGTGGGAGCCGCGGGGGACGCGCC AGCCCCGGCCCCCAACAAGGACGGAGACGCCGGCGTGGGCAGCGGCCACTGGGAGCTGAGGTGCCATCGC CTGCAGGATTCTTTATTCAGCTCTGACAGTGGCTTCAGCAACTACCGTGGCATCCTGAACTGGTGTGTGG TGATGCTGATCTTGAGCAATGCCCGGTTATTTCTGGAGAACCTCATCAAGTATGGCATCCTGGTGGACCC CATCCAGGTGGTTTCTCTGTTCCTGAAGGATCCCTATAGCTGGCCCGCCCCATGCCTGGTTATTGCGGCC AATGTCTTTGCTGTGGCTGCATTCCAGGTTGAGAAGCGCCTGGCGGTGGGTGCCCTGACGGAGCAGGCGG GACTGCTGCTGCACGTGGCCAACCTGGCCACCATTCTGTGTTTCCCAGCGGCTGTGGTCTTACTGGTTGA GTCTATCACTCCAGTGGGCTCCCTGCTGGCGCTGATGGCGCACACCATCCTCTTCCTCAAGCTCTTCTCC TACCGCGACGTCAACTCATGGTGCCGCAGGGCCAGGGCCAAGGCTGCCTCTGCAGGGAAGAAGGCCAGCA GTGCTGCTGCCCCGCACACCGTGAGCTACCCGGACAATCTGACCTACCGCGATCTCTACTACTTCCTCTT CGCCCCCACCTTGTGCTACGAGCTCAACTTTCCCCGCTCTCCCCGCATCCGGAAGCGCTTTCTGCTGCGA CGGATCCTTGAGATGCTGTTCTTCACCCAGCTCCAGGTGGGGCTGATCCAGCAGTGGATGGTCCCCACCA TCCAGAACTCCATGAAGCCCTTCAAGGACATGGACTACTCACGCATCATCGAGCGCCTCCTGAAGCTGGC GGTCCCCAATCACCTCATCTGGCTCATCTTCTTCTACTGGCTCTTCCACTCCTGCCTGAATGCCGTGGCT GAGCTCATGCAGTTTGGAGACCGGGAGTTCTACCGGGACTGGTGGAACTCCGAGTCTGTCACCTACTTCT GGCAGAACTGGAACATCCCTGTGCACAAGTGGTGCATCAGACACTTCTACAAGCCCATGCTTCGACGGGG CAGCAGCAAGTGGATGGCCAGGACAGGGGTGTTCCTGGCCTCGGCCTTCTTCCACGAGTACCTGGTGAGC GTCCCTCTGCGAATGTTCCGCCTCTGGGCGTTCACGGGCATGATGGCTCAGATCCCACTGGCCTGGTTCG TGGGCCGCTTTTTCCAGGGCAACTATGGCAACGCAGCTGTGTGGCTGTCGCTCATCATCGGACAGCCAAT AGCCGTTCTCATGTACGTCCACGACTACTACGTGCTCAACTATGAGGCCCCAGCGGCAGAGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220595 representing NM_012079
Red=Cloning site Green=Tags(s) MGDRGSSRRRRTGSRPSSHGGGGPAAAEEEVRDAAAGPDVGAAGDAPAPAPNKDGDAGVGSGHWELRCHR LQDSLFSSDSGFSNYRGILNWCVVMLILSNARLFLENLIKYGILVDPIQVVSLFLKDPYSWPAPCLVIAA NVFAVAAFQVEKRLAVGALTEQAGLLLHVANLATILCFPAAVVLLVESITPVGSLLALMAHTILFLKLFS YRDVNSWCRRARAKAASAGKKASSAAAPHTVSYPDNLTYRDLYYFLFAPTLCYELNFPRSPRIRKRFLLR RILEMLFFTQLQVGLIQQWMVPTIQNSMKPFKDMDYSRIIERLLKLAVPNHLIWLIFFYWLFHSCLNAVA ELMQFGDREFYRDWWNSESVTYFWQNWNIPVHKWCIRHFYKPMLRRGSSKWMARTGVFLASAFFHEYLVS VPLRMFRLWAFTGMMAQIPLAWFVGRFFQGNYGNAAVWLSLIIGQPIAVLMYVHDYYVLNYEAPAAEA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012079 |
| ORF Size | 1464 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012079.6 |
| RefSeq Size | 1976 bp |
| RefSeq ORF | 1467 bp |
| Locus ID | 8694 |
| UniProt ID | O75907 |
| Domains | MBOAT |
| Protein Families | Transmembrane |
| Protein Pathways | Glycerolipid metabolism, Metabolic pathways, Retinol metabolism |
| MW | 55.1 kDa |
| Gene Summary | This gene encodes an multipass transmembrane protein that functions as a key metabolic enzyme. The encoded protein catalyzes the conversion of diacylglycerol and fatty acyl CoA to triacylglycerol. This enzyme can also transfer acyl CoA to retinol. Activity of this protein may be associated with obesity and other metabolic diseases. [provided by RefSeq, Jul 2013] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Discovery and optimization of adamantane carboxylic acid derivatives as potent diacylglycerol acyltransferase 1 inhibitors for the potential treatment of obesity and diabetes
,Pagire, SH;Pagire, HS;Lee, GB;Han, SJ;Kwak, HJ;Kim, JY;Kim, KY;Rhee, SD;Ryu, JI;Song, JS;Bae, MA;Park, MJ;Kim, D;Lee, DH;Ahn, JH;,
Eur J Med Chem
,PubMed ID 26218650
[DGAT1]
|
|
Synthesis and Diacylglycerol Acyltransferase-1 Inhibition of Azabicyclo [3.1. 0] hexane Derivatives
,Han, SJ;Lee, GB;Kwak, HJ;Pagire, SH;,
BULLETIN OF THEKOREAN CHEMICAL SOCIETY
[DGAT1]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220595L1 | Lenti-ORF clone of DGAT1 (Myc-DDK-tagged)-Human diacylglycerol O-acyltransferase 1 (DGAT1) |
CNY 6,056.00 |
|
| RC220595L2 | Lenti-ORF clone of DGAT1 (mGFP-tagged)-Human diacylglycerol O-acyltransferase 1 (DGAT1) |
CNY 5,890.00 |
|
| RC220595L3 | Lenti-ORF clone of DGAT1 (Myc-DDK-tagged)-Human diacylglycerol O-acyltransferase 1 (DGAT1) |
CNY 5,890.00 |
|
| RC220595L4 | Lenti-ORF clone of DGAT1 (mGFP-tagged)-Human diacylglycerol O-acyltransferase 1 (DGAT1) |
CNY 6,056.00 |
|
| RG220595 | DGAT1 (tGFP-tagged) - Human diacylglycerol O-acyltransferase 1 (DGAT1) |
CNY 5,256.00 |
|
| SC115583 | DGAT1 (untagged)-Human diacylglycerol O-acyltransferase 1 (DGAT1) |
CNY 3,656.00 |
