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Protein kinase like protein SgK493 (PKDCC) (NM_138370) Human Tagged ORF Clone
CAT＃: RC220604
PKDCC (Myc-DDK-tagged)-Human protein kinase domain containing, cytoplasmic homolog (mouse) (PKDCC)
ORF Plasmid: tGFP
"NM_138370" in other vectors (7)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RLSDF; SGK493; Vlk |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220604 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCTGCTGGAGCGGCTGCGGCACCCCAACGTGCTGCAGCTCTATGGCTACTGCTACCAGGACAGCG AGGACATCCCAGACACCCTGACCACCATCACGGAGCTGGGCGCCCCTGTAGAAATGATCCAGCTGCTGCA AACTTCCTGGGAGGATCGATTCCGAATCTGCCTGAGCCTGGGCCGCCTCCTCCACCACCTGGCCCACTCC CCACTGGGCTCCGTCACTCTGCTGGACTTCCGCCCTCGGCAGTTTGTGCTGGTGGATGGGGAGCTCAAAG TGACGGACCTGGATGACGCACGTGTGGAGGAGACGCCGTGTGCAGGCAGCACCGACTGCATACTCGAGTT TCCGGCCAGGAACTTCACCCTGCCCTGCTCAGCCCAGGGCTGGTGCGAGGGCATGAACGAGAAGCGGAAC CTCTATAATGCCTACAGGTTTTTCTTCACATACCTCCTGCCTCACAGTGCCCCGCCTTCACTGCGTCCTC TGCTGGACAGCATCGTCAACGCCACAGGAGAGCTCGCCTGGGGGGTGGACGAGACCCTGGCCCAGCTGGA GAAGGTGCTGCACCTGTACCGGAGCGGGCAGTATCTGCAGAACTCCACGGCAAGCAGCAGTACCGAGTAC CAGTGTATCCCAGACAGCACCATCCCCCAGGAAGACTACCGCTGCTGGCCATCCTACCACCACGGGAGCT GCCTCCTTTCAGTGTTCAACCTGGCTGAGGCTGTGGATGTCTGTGAGAGCCATGCCCAGTGTCGGGCCTT TGTGGTCACCAACCAGACCACCTGGACAGGTCGGCAGCTGGTCTTTTTCAAGACTGGATGGAGCCAAGTG GTCCCTGATCCCAACAAGACCACATATGTGAAGGCCTCTGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220604 protein sequence
Red=Cloning site Green=Tags(s) MVLLERLRHPNVLQLYGYCYQDSEDIPDTLTTITELGAPVEMIQLLQTSWEDRFRICLSLGRLLHHLAHS PLGSVTLLDFRPRQFVLVDGELKVTDLDDARVEETPCAGSTDCILEFPARNFTLPCSAQGWCEGMNEKRN LYNAYRFFFTYLLPHSAPPSLRPLLDSIVNATGELAWGVDETLAQLEKVLHLYRSGQYLQNSTASSSTEY QCIPDSTIPQEDYRCWPSYHHGSCLLSVFNLAEAVDVCESHAQCRAFVVTNQTTWTGRQLVFFKTGWSQV VPDPNKTTYVKASG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138370 |
| ORF Size | 882 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_138370.1, NP_612379.1 |
| RefSeq Size | 2517 bp |
| RefSeq ORF | 1482 bp |
| Locus ID | 91461 |
| UniProt ID | Q504Y2 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 33.3 kDa |
| Gene Summary | Secreted tyrosine-protein kinase that mediates phosphorylation of extracellular proteins and endogenous proteins in the secretory pathway, which is essential for patterning at organogenesis stages. Mediates phosphorylation of MMP1, MMP13, MMP14, MMP19 and ERP29 (PubMed:25171405). Probably plays a role in platelets: rapidly and quantitatively secreted from platelets in response to stimulation of platelet degranulation (PubMed:25171405). May also have serine/threonine protein kinase activity. Required for longitudinal bone growth through regulation of chondrocyte differentiation. May be indirectly involved in protein transport from the Golgi apparatus to the plasma membrane (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220604L1 | Lenti ORF clone of Human protein kinase domain containing, cytoplasmic homolog (mouse) (PKDCC), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC220604L2 | Lenti ORF clone of Human protein kinase domain containing, cytoplasmic homolog (mouse) (PKDCC), mGFP tagged |
CNY 5,890.00 |
|
| RC220604L3 | Lenti ORF clone of Human protein kinase domain containing, cytoplasmic homolog (mouse) (PKDCC), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC220604L4 | Lenti ORF clone of Human protein kinase domain containing, cytoplasmic homolog (mouse) (PKDCC), mGFP tagged |
CNY 5,890.00 |
|
| RG220604 | PKDCC (tGFP-tagged) - Human protein kinase domain containing, cytoplasmic homolog (mouse) (PKDCC) |
CNY 4,000.00 |
|
| SC107086 | PKDCC (untagged)-Human protein kinase domain containing, cytoplasmic homolog (mouse) (PKDCC) |
CNY 7,890.00 |
|
| SC327880 | PKDCC (untagged)-Human protein kinase-like protein SgK493 (SGK493) |
CNY 3,656.00 |
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