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SPRED1 (NM_152594) Human Tagged Lenti ORF Clone
CAT＃: RC220714L3
- LentiORF®
Lenti ORF clone of Human sprouty-related, EVH1 domain containing 1 (SPRED1), Myc-DDK-tagged
"NM_152594" in other vectors (6)
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CNY 5,890.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | hSpred1; LGSS; NFLS; PPP1R147; spred-1 |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(RC220714).
|
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NM_152594 |
| ORF Size | 1332 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_152594.1, NP_689807.1 |
| RefSeq Size | 3816 bp |
| RefSeq ORF | 1335 bp |
| Locus ID | 161742 |
| UniProt ID | Q7Z699 |
| Domains | WH1 |
| Protein Pathways | Jak-STAT signaling pathway |
| MW | 50.3 kDa |
| Gene Summary | The protein encoded by this gene is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220714 | SPRED1 (Myc-DDK-tagged)-Human sprouty-related, EVH1 domain containing 1 (SPRED1) |
CNY 3,656.00 |
|
| RC220714L1 | Lenti ORF clone of Human sprouty-related, EVH1 domain containing 1 (SPRED1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC220714L2 | Lenti ORF clone of Human sprouty-related, EVH1 domain containing 1 (SPRED1), mGFP tagged |
CNY 5,890.00 |
|
| RC220714L4 | Lenti ORF clone of Human sprouty-related, EVH1 domain containing 1 (SPRED1), mGFP tagged |
CNY 5,890.00 |
|
| RG220714 | SPRED1 (tGFP-tagged) - Human sprouty-related, EVH1 domain containing 1 (SPRED1) |
CNY 4,370.00 |
|
| SC101247 | SPRED1 (untagged)-Human sprouty-related, EVH1 domain containing 1 (SPRED1) |
CNY 3,656.00 |
