KCNG4 (NM_172347) Human Tagged ORF Clone

CAT#: RC220851

KCNG4 (Myc-DDK-tagged)-Human potassium voltage-gated channel, subfamily G, member 4 (KCNG4)

ORF Plasmid: DDK tGFP



  "NM_172347" in other vectors (4)

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CNY 3,864.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-KCNG4 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms KV6.3; KV6.4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC220851 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCATGCCTTCCAGAGACGGGGGCCTGCATCCCAGACACCACCACTATGGTTCCCACAGCCCTTGGA
GTCAGCTCCTGTCCAGCCCCATGGAGACGCCGTCCATCAAGGGCCTTTACTACCGGAGGGTGCGGAAGGT
GGGTGCCCTGGACGCCTCCCCAGTGGACCTGAAGAAGGAGATCCTGATCAACGTGGGGGGCAGGAGGTAT
CTCCTCCCCTGGAGCACACTGGACCGGTTCCCGCTGAGCCGCCTGAGCAAACTCAGGCTCTGTCGGAGCT
ACGAGGAGATCGTGCAGCTCTGCGATGATTACGACGAGGACAGCCAGGAGTTCTTCTTCGACAGGAGCCC
CAGCGCCTTCGGGGTGATCGTGAGCTTCCTGGCGGCCGGGAAGCTGGTGCTTCTGCAGGAGATGTGCGCG
CTGTCCTTCCAGGAGGAGCTGGCCTACTGGGGCATCGAGGAGGCCCACCTGGAGAGGTGCTGCCTGCGGA
AGCTGCTGAGGAAGCTGGAGGAGCTGGAGGAGCTGGCCAAGCTGCACAGGGAGGACGTACTGAGGCAGCA
GAGGGAGACCCGCCGCCCCGCCTCGCACTCCTCGCGCTGGGGCCTGTGCATGAACCGGCTGCGCGAGATG
GTGGAAAACCCGCAGTCCGGGCTGCCCGGGAAGGTCTTCGCTTGCCTCTCCATCCTCTTCGTGGCCACCA
CAGCCGTCAGCCTGTGTGTCAGCACCATGCCCGACCTCAGGGCAGAGGAGGACCAGGGCGAATGCTCTCG
GAAGTGCTACTATATTTTCATCGTGGAGACCATCTGCGTGGCCTGGTTCTCCCTGGAGTTCTGCCTGCGG
TTTGTCCAGGCCCAAGACAAGTGTCAGTTCTTCCAGGGGCCCCTGAACATCATCGACATCCTGGCCATCT
CCCCATACTACGTGTCGCTGGCGGTGTCTGAGGAGCCCCCGGAGGACGGCAAGAGGCCGAGCGGGAGCTC
CTACCTGGAGAAGGTGGGGCTGGTCCTGCGTGTGCTGCGAGCGCTGCGCATCCTCTACGTGATGCGCCTG
GCTCGCCACTCGCTGGGGCTGCAGACGCTGGGGCTCACCGTGCGCCGTTGCACACGTGAGTTCGGCCTGC
TCCTTCTCTTCCTGGCCGTGGCCATCACCCTCTTCTCCCCTTTGGTCTACGTGGCCGAGAAGGAGTCCGG
GCGGGTGCTGGAGTTCACCAGCATCCCCGCCTCCTATTGGTGGGCCATCATCTCCATGACAACGGTGGGC
TACGGGGACATGGTGCCCCACAGTGTGCCAGGCCAGATGGTGGCCCTCAGCAGCATCCTGAGCGGGATCC
TCATCATGGCCTTCCCGGCCACGTCTATCTTCCACACCTTCTCCCACTCCTACCTGGAGCTCAAGAAGGA
GCAGGAGCAGCTTCAGGCCCGCCTCCGCCACCTCCAAAACACCGGTCCAGCCAGTGAATGTGAACTCCTG
GACCCCCATGTGGCCAGTGAACATGAGCTCATGAACGATGTCAATGACCTAATCCTGGAGGGCCCAGCCT
TGCCTATCATGCACATGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC220851 protein sequence
Red=Cloning site Green=Tags(s)

MPMPSRDGGLHPRHHHYGSHSPWSQLLSSPMETPSIKGLYYRRVRKVGALDASPVDLKKEILINVGGRRY
LLPWSTLDRFPLSRLSKLRLCRSYEEIVQLCDDYDEDSQEFFFDRSPSAFGVIVSFLAAGKLVLLQEMCA
LSFQEELAYWGIEEAHLERCCLRKLLRKLEELEELAKLHREDVLRQQRETRRPASHSSRWGLCMNRLREM
VENPQSGLPGKVFACLSILFVATTAVSLCVSTMPDLRAEEDQGECSRKCYYIFIVETICVAWFSLEFCLR
FVQAQDKCQFFQGPLNIIDILAISPYYVSLAVSEEPPEDGKRPSGSSYLEKVGLVLRVLRALRILYVMRL
ARHSLGLQTLGLTVRRCTREFGLLLLFLAVAITLFSPLVYVAEKESGRVLEFTSIPASYWWAIISMTTVG
YGDMVPHSVPGQMVALSSILSGILIMAFPATSIFHTFSHSYLELKKEQEQLQARLRHLQNTGPASECELL
DPHVASEHELMNDVNDLILEGPALPIMHM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_172347
ORF Size 1558 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_172347.2, NP_758857.1
RefSeq Size 2779 bp
RefSeq ORF 1560 bp
Locus ID 93107
UniProt ID Q8TDN1
Protein Families Druggable Genome, Ion Channels: Potassium, Transmembrane
MW 59 kDa
Gene Summary Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, subfamily G. This member functions as a modulatory subunit. The gene has strong expression in brain. Multiple alternatively spliced variants have been found in normal and cancerous tissues. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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