PDE7A (NM_002604) Human Tagged ORF Clone
CAT#: RC220940
PDE7A (Myc-DDK-tagged)-Human phosphodiesterase 7A (PDE7A), transcript variant 2
ORF Plasmid: tGFP
"NM_002604" in other vectors (4)
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CNY 3,656.00
CNY 4,280.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HCP1; PDE7 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC220940 representing NM_002604
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGTGTGTTACCAGCTGCCGGTACTGCCCCTGGACAGGCCGGTCCCCCAGCACGTCCTCAGCCGCC GAGGAGCCATCAGCTTCAGCTCCAGCTCCGCTCTCTTCGGCTGCCCCAATCCCCGGCAGCTCTCTCAGAG GCGTGGAGCTATTTCCTATGACAGTTCTGATCAGACTGCATTATACATTCGTATGCTAGGAGATGTACGT GTAAGGAGCCGAGCAGGATTTGAATCAGAAAGAAGAGGTTCTCACCCATATATTGATTTTCGTATTTTCC ACTCTCAATCTGAAATTGAAGTGTCTGTCTCTGCAAGGAATATCAGAAGGCTACTAAGTTTCCAGCGATA TCTTAGATCTTCACGCTTTTTTCGTGGTACTGCGGTTTCAAATTCCCTAAACATTTTAGATGATGATTAT AATGGACAAGCCAAGTGTATGCTGGAAAAAGTTGGAAATTGGAATTTTGATATCTTTCTATTTGATAGAC TAACAAATGGAAATAGTCTAGTAAGCTTAACCTTTCATTTATTTAGTCTTCATGGATTAATTGAGTACTT CCATTTAGATATGATGAAACTTCGTAGATTTTTAGTTATGATTCAAGAAGATTACCACAGTCAAAATCCT TACCATAACGCAGTCCACGCTGCGGATGTTACTCAGGCCATGCACTGTTACTTAAAGGAACCTAAGCTTG CCAATTCTGTAACTCCTTGGGATATCTTGCTGAGCTTAATTGCAGCTGCCACTCATGATCTGGATCATCC AGGTGTTAATCAACCTTTCCTTATTAAAACTAACCATTACTTGGCAACTTTATACAAGAATACCTCAGTA CTGGAAAATCACCACTGGAGATCTGCAGTGGGCTTATTGAGAGAATCAGGCTTATTCTCACATCTGCCAT TAGAAAGCAGGCAACAAATGGAGACACAGATAGGTGCTCTGATACTAGCCACAGACATCAGTCGCCAGAA TGAGTATCTGTCTTTGTTTAGGTCCCATTTGGATAGAGGTGATTTATGCCTAGAAGACACCAGACACAGA CATTTGGTTTTACAGATGGCTTTGAAATGTGCTGATATTTGTAACCCATGTCGGACGTGGGAATTAAGCA AGCAGTGGAGTGAAAAAGTAACGGAGGAATTCTTCCATCAAGGAGATATAGAAAAAAAATATCATTTGGG TGTGAGTCCACTTTGCGATCGTCACACTGAATCTATTGCCAACATCCAGATTGGTAACTATACATATTTA GATATAGCTGGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220940 representing NM_002604
Red=Cloning site Green=Tags(s) MEVCYQLPVLPLDRPVPQHVLSRRGAISFSSSSALFGCPNPRQLSQRRGAISYDSSDQTALYIRMLGDVR VRSRAGFESERRGSHPYIDFRIFHSQSEIEVSVSARNIRRLLSFQRYLRSSRFFRGTAVSNSLNILDDDY NGQAKCMLEKVGNWNFDIFLFDRLTNGNSLVSLTFHLFSLHGLIEYFHLDMMKLRRFLVMIQEDYHSQNP YHNAVHAADVTQAMHCYLKEPKLANSVTPWDILLSLIAAATHDLDHPGVNQPFLIKTNHYLATLYKNTSV LENHHWRSAVGLLRESGLFSHLPLESRQQMETQIGALILATDISRQNEYLSLFRSHLDRGDLCLEDTRHR HLVLQMALKCADICNPCRTWELSKQWSEKVTEEFFHQGDIEKKYHLGVSPLCDRHTESIANIQIGNYTYL DIAG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002604 |
ORF Size | 1272 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002604.2 |
RefSeq Size | 2990 bp |
RefSeq ORF | 1274 bp |
Locus ID | 5150 |
Domains | PDEase, HDc |
Protein Families | Druggable Genome |
Protein Pathways | Progesterone-mediated oocyte maturation, Purine metabolism |
MW | 48.6 kDa |
Gene Summary | The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE7 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC220940L3 | Lenti ORF clone of Human phosphodiesterase 7A (PDE7A), transcript variant 2, Myc-DDK-tagged |
CNY 6,180.00 |
|
RC220940L4 | Lenti ORF clone of Human phosphodiesterase 7A (PDE7A), transcript variant 2, mGFP tagged |
CNY 6,180.00 |
|
RG220940 | PDE7A (tGFP-tagged) - Human phosphodiesterase 7A (PDE7A), transcript variant 2 |
CNY 5,256.00 |
|
SC313399 | PDE7A (untagged)-Human phosphodiesterase 7A (PDE7A), transcript variant 2 |
CNY 3,656.00 |