United States
Germany
Japan
United Kingdom
China
PDE4C (NM_001098819) Human Tagged ORF Clone
CAT＃: RC221103
PDE4C (Myc-DDK-tagged)-Human phosphodiesterase 4C, cAMP-specific (PDE4C), transcript variant 2
ORF Plasmid: tGFP
"NM_001098819" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,512.00
CNY 4,560.00
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DPDE1; PDE21 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221103 representing NM_001098819
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGCTCCAGTCCCGCACAGCCAGCGGCGCGAGTCCTTCCTGTACCGCTCAGATAGCGACTATGAAC TCTCGCCCAAGGCCATGTCTCGGAACTCCTCTGTGGCCAGCGACCTACATGGAGAGGACATGATTGTGAC GCCCTTTGCCCAGGTCCTGGCCAGTCTGCGGACCGTTCGGAGCAACGTGGCGGCCCTTGCCCGCCAGCAA TGCCTAGGAGCAGCCAAGCAGGGACCCGTCGGAAACCCTTCATCCAGCAATCAGCTCCCTCCTGCAGAGG ACACGGGGCAGAAGCTGGCATTGGAGACGCTAGACGAGCTGGACTGGTGCCTGGATCAGTTGGAGACGCT GCAGACCCGGCACTCGGTGGGGGAGATGGCCTCCAACAAGTTCAAGCGGATCCTGAACCGGGAGTTGACC CACCTGTCCGAAACCAGCCGCTCCGGGAACCAGGTGTCCGAGTACATCTCCCGGACCTTCCTGGACCAGC AGACCGAGGTGGAGCTGCCCAAGGTGACCGCTGAGGAGGCCCCACAGCCCATGTCCCGGATCAGTGGCCT ACATGGGCTCTGCCACAGTGCCAGCCTCTCCTCAGCCACTGTCCCACGCTTTGGGGTCCAGACTGACCAG GAGGAGCAACTGGCCAAGGAGCTAGAAGACACCAACAAGTGGGGACTTGATGTGTTCAAGGTGGCGGAGC TAAGTGGGAACCGGCCCCTCACAGCTATCATATTCAGCATTTTTCAGGAGCGGGACCTGCTGAAGACATT CCAGATCCCAGCAGACACACTGGCCACCTACCTGCTGATGCTGGAAGGTCACTACCACGCCAATGTGGCC TACCACAACAGCCTACATGCCGCCGACGTGGCCCAGTCCACGCATGTGCTGCTGGCTACGCCCGCCCTCG AGGCTGTGTTCACAGACTTGGAAATCCTGGCTGCCCTCTTTGCAAGCGCCATCCACGACGTGGACCATCC TGGGGTCTCCAACCAGTTTCTGATTAACACCAACTCAGAGCTGGCGCTTATGTACAACGACGCCTCGGTG CTGGAGAACCATCACCTGGCTGTGGGCTTCAAGCTGCTGCAGGCAGAGAACTGCGATATCTTCCAGAACC TCAGCGCCAAGCAGCGACTGAGTCTGCGCAGGATGGTCATTGACATGGTGCTGGCCACAGACATGTCCAA ACACATGAACCTCCTGGCCGACCTCAAGACCATGGTGGAGACCAAGAAGGTGACAAGCCTCGGTGTCCTC CTCCTGGACAACTATTCCGACCGAATCCAGGTCTTGCAGAACCTGGTGCACTGTGCTGATCTGAGCAACC CCACCAAGCCGCTGCCCCTGTACCGCCAGTGGACGGACCGCATCATGGCCGAGTTCTTCCAGCAGGGAGA CCGCGAGCGTGAGTCGGGCCTGGACATCAGTCCCATGTGTGACAAGCATACGGCCTCAGTGGAGAAGTCC CAGGTGGGTTTCATTGACTACATTGCTCACCCACTGTGGGAGACTTGGGCTGACCTGGTCCACCCAGATG CACAGGACCTGCTGGACACGCTGGAGGACAATCGAGAGTGGTACCAGAGCAAGATCCCCCGAAGTCCCTC AGACCTCACCAACCCCGAGCGGGACGGGCCTGACAGATTCCAGTTTGAACTGACTCTGGAGGAGGCAGAG GAAGAGGATGAGGAGGAAGAAGAGGAGGGGGAAGAGACAGCTTTAGCCAAAGAGGCCTTGGAGTTGCCTG ACACTGAACTCCTGTCCCCTGAAGCCGGCCCAGACCCTGGGGACTTACCCCTCGACAACCAGAGGACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221103 representing NM_001098819
Red=Cloning site Green=Tags(s) MQAPVPHSQRRESFLYRSDSDYELSPKAMSRNSSVASDLHGEDMIVTPFAQVLASLRTVRSNVAALARQQ CLGAAKQGPVGNPSSSNQLPPAEDTGQKLALETLDELDWCLDQLETLQTRHSVGEMASNKFKRILNRELT HLSETSRSGNQVSEYISRTFLDQQTEVELPKVTAEEAPQPMSRISGLHGLCHSASLSSATVPRFGVQTDQ EEQLAKELEDTNKWGLDVFKVAELSGNRPLTAIIFSIFQERDLLKTFQIPADTLATYLLMLEGHYHANVA YHNSLHAADVAQSTHVLLATPALEAVFTDLEILAALFASAIHDVDHPGVSNQFLINTNSELALMYNDASV LENHHLAVGFKLLQAENCDIFQNLSAKQRLSLRRMVIDMVLATDMSKHMNLLADLKTMVETKKVTSLGVL LLDNYSDRIQVLQNLVHCADLSNPTKPLPLYRQWTDRIMAEFFQQGDRERESGLDISPMCDKHTASVEKS QVGFIDYIAHPLWETWADLVHPDAQDLLDTLEDNREWYQSKIPRSPSDLTNPERDGPDRFQFELTLEEAE EEDEEEEEEGEETALAKEALELPDTELLSPEAGPDPGDLPLDNQRT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001098819 |
| ORF Size | 1818 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001098819.3 |
| RefSeq Size | 5124 bp |
| RefSeq ORF | 1821 bp |
| Locus ID | 5143 |
| UniProt ID | Q08493 |
| Protein Families | Druggable Genome |
| Protein Pathways | Progesterone-mediated oocyte maturation, Purine metabolism |
| MW | 67.6 kDa |
| Gene Summary | The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221103L3 | Lenti ORF clone of Human phosphodiesterase 4C, cAMP-specific (PDE4C), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC221103L4 | Lenti ORF clone of Human phosphodiesterase 4C, cAMP-specific (PDE4C), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RG221103 | PDE4C (tGFP-tagged) - Human phosphodiesterase 4C, cAMP-specific (PDE4C), transcript variant 2 |
CNY 5,040.00 |
|
| SC316553 | PDE4C (untagged)-Human phosphodiesterase 4C, cAMP-specific (PDE4C), transcript variant 2 |
CNY 9,790.00 |
