A4GNT (NM_016161) Human Tagged ORF Clone

CAT#: RC221256

A4GNT (Myc-DDK-tagged)-Human alpha-1,4-N-acetylglucosaminyltransferase (A4GNT)

ORF Plasmid: DDK tGFP



  "NM_016161" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


A4GNT mouse monoclonal antibody, clone OTI5B5 (formerly 5B5)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms alpha4GnT
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC221256 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGAAGGAGCTCCAGCTCTCCCTGTCAGTCACCTTGCTGCTTGTCTGTGGCTTCCTCTACCAGTTCA
CCCTGAAGTCCAGCTGCCTCTTCTGCTTGCCTTCTTTCAAGTCCCACCAGGGGCTGGAAGCCCTCCTGAG
CCACAGACGTGGCATTGTGTTTCTAGAGACCTCAGAGAGAATGGAGCCACCCCATTTGGTCTCCTGTTCC
GTAGAGTCTGCTGCCAAGATTTATCCTGAGTGGCCTGTGGTGTTCTTTATGAAGGGTCTTACTGATTCCA
CACCGATGCCCTCAAACTCCACATACCCAGCTTTTTCCTTCCTGTCAGCAATAGACAACGTTTTCCTCTT
CCCTTTGGATATGAAAAGGCTGCTTGAAGACACACCATTGTTTTCATGGTACAATCAAATCAACGCCAGC
GCAGAGAGAAACTGGCTCCACATCAGCTCGGATGCATCCCGCCTGGCCATCATCTGGAAATACGGTGGCA
TCTACATGGACACCGATGTCATCTCCATCAGGCCCATCCCTGAGGAGAACTTTTTGGCTGCGCAGGCTTC
TCGGTACTCTAGTAATGGAATATTTGGGTTCCTCCCCCACCACCCCTTTTTGTGGGAATGCATGGAAAAC
TTTGTTGAACACTATAATTCAGACATTTGGGGCAACCAAGGCCCTGAGTTGATGACAAGGATGTTGAGGG
TATGGTGTAAACTTGAAGACTTCCAGGAGGTGAGCGACCTCAGGTGTCTGAACATATCCTTCTTACACCC
CCAAAGATTTTACCCCATCTCCTATCGAGAGTGGAGGCGCTACTATGAAGTGTGGGATACAGAGCCAAGC
TTCAATGTCTCTTATGCCCTGCATTTGTGGAACCACATGAACCAGGAGGGGCGGGCTGTGATTAGAGGAA
GCAACACACTGGTGGAAAATCTCTATCGCAAGCACTGTCCCAGGACTTACAGGGACCTGATTAAAGGCCC
AGAGGGGTCAGTGACTGGGGAGCTGGGTCCAGGTAACAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC221256 protein sequence
Red=Cloning site Green=Tags(s)

MRKELQLSLSVTLLLVCGFLYQFTLKSSCLFCLPSFKSHQGLEALLSHRRGIVFLETSERMEPPHLVSCS
VESAAKIYPEWPVVFFMKGLTDSTPMPSNSTYPAFSFLSAIDNVFLFPLDMKRLLEDTPLFSWYNQINAS
AERNWLHISSDASRLAIIWKYGGIYMDTDVISIRPIPEENFLAAQASRYSSNGIFGFLPHHPFLWECMEN
FVEHYNSDIWGNQGPELMTRMLRVWCKLEDFQEVSDLRCLNISFLHPQRFYPISYREWRRYYEVWDTEPS
FNVSYALHLWNHMNQEGRAVIRGSNTLVENLYRKHCPRTYRDLIKGPEGSVTGELGPGNK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016161
ORF Size 1020 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016161.3
RefSeq Size 1771 bp
RefSeq ORF 1023 bp
Locus ID 51146
UniProt ID Q9UNA3
Protein Families Transmembrane
MW 39.5 kDa
Gene Summary This gene encodes a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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