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GPR48 (LGR4) (NM_018490) Human Tagged ORF Clone
CAT＃: RC221345
LGR4 (Myc-DDK-tagged)-Human leucine-rich repeat containing G protein-coupled receptor 4 (LGR4)
ORF Plasmid: tGFP
"NM_018490" in other vectors (6)
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CNY 10,448.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BNMD17; GPR48 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221345 representing NM_018490
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGGCCCGCTAGGGCTGCTCTGCTTCCTCGCCCTGGGGCTGCTCGGCTCGGCCGGGCCCAGCGGCG CGGCGCCGCCTCTCTGCGCGGCGCCCTGCAGCTGCGACGGCGACCGTCGGGTGGACTGCTCCGGGAAGGG GCTGACGGCCGTGCCCGAGGGGCTCAGCGCCTTCACCCAAGCGCTGGATATCAGTATGAACAACATTACT CAGTTGCCAGAAGATGCATTTAAGAACTTTCCTTTTCTAGAAGAGCTACAATTGGCGGGCAACGACCTTT CTTTTATCCACCCAAAGGCCTTGTCTGGGTTGAAAGAACTCAAAGTTCTAACGCTCCAGAATAATCAGTT GAAAACAGTACCCAGTGAAGCCATTCGAGGGCTGAGTGCTTTGCAGTCTTTGCGTTTAGATGCCAACCAT ATTACCTCAGTCCCCGAGGACAGTTTTGAAGGACTTGTTCAGTTACGGCATCTGTGGCTGGATGACAACA GCTTGACGGAGGTGCCTGTGCACCCCCTCAGCAATCTGCCCACCCTACAGGCGCTGACCCTGGCTCTCAA CAAGATCTCAAGCATCCCTGACTTTGCATTTACCAACCTTTCAAGCCTGGTAGTTCTGCATCTTCATAAC AATAAAATTAGAAGCCTGAGTCAACACTGTTTTGATGGACTAGATAACCTGGAGACCTTAGACTTGAATT ATAATAACTTGGGGGAATTTCCTCAGGCTATTAAAGCCCTTCCTAGCCTTAAAGAGCTAGGATTTCATAG TAATTCTATTTCTGTTATCCCTGATGGAGCATTTGATGGTAATCCACTCTTAAGAACTATACATTTGTAT GATAATCCTCTGTCTTTTGTGGGGAACTCAGCATTTCACAATTTATCTGATCTTCATTCCCTAGTCATTC GTGGTGCAAGCATGGTGCAGCAGTTCCCCAATCTTACAGGAACTGTCCACCTGGAAAGTCTGACTTTGAC AGGTACAAAGATAAGCAGCATACCTAATAATTTGTGTCAAGAACAAAAGATGCTTAGGACTTTGGACTTG TCTTACAATAATATAAGAGACCTTCCAAGTTTTAATGGTTGCCATGCTCTGGAAGAAATTTCTTTACAGC GTAATCAAATCTACCAAATAAAGGAAGGCACCTTTCAAGGCCTGATATCTCTAAGGATTCTAGATCTGAG TAGAAACCTGATACATGAAATTCACAGTAGAGCTTTTGCCACACTTGGGCCAATAACTAACCTAGATGTA AGTTTCAATGAATTAACTTCCTTTCCTACGGAAGGCCTGAATGGGCTAAATCAACTGAAACTTGTGGGCA ACTTCAAGCTGAAAGAAGCCTTAGCAGCAAAAGACTTTGTTAACCTCAGGTCTTTATCAGTACCATATGC TTATCAGTGCTGTGCATTTTGGGGTTGTGACTCTTATGCAAATTTAAACACAGAAGATAACAGCCTCCAG GACCACAGTGTGGCACAGGAGAAAGGTACTGCTGATGCAGCAAATGTCACAAGCACTCTTGAAAATGAAG AACATAGTCAAATAATTATCCATTGTACACCTTCAACAGGTGCTTTTAAGCCCTGTGAATATTTACTGGG AAGCTGGATGATTCGTCTTACTGTGTGGTTCATTTTCTTGGTTGCATTATTTTTCAACCTGCTTGTTATT TTAACAACATTTGCATCTTGTACATCACTGCCTTCGTCCAAATTGTTTATAGGCTTGATTTCTGTGTCTA ACTTATTCATGGGAATCTATACTGGCATCCTAACTTTTCTTGATGCTGTGTCCTGGGGCAGATTCGCTGA ATTTGGCATTTGGTGGGAAACTGGCAGTGGCTGCAAAGTAGCTGGGTTTCTTGCAGTTTTCTCCTCAGAA AGTGCCATATTTTTATTAATGCTAGCAACTGTCGAAAGAAGCTTATCTGCAAAAGATATAATGAAAAATG GGAAGAGCAATCATCTCAAACAGTTCCGGGTTGCTGCCCTTTTGGCTTTCCTAGGTGCTACAGTAGCAGG CTGTTTTCCCCTTTTCCATAGAGGGGAATATTCTGCATCACCCCTTTGTTTGCCATTTCCTACAGGTGAA ACGCCATCATTAGGATTCACTGTAACGTTAGTGCTATTAAACTCACTAGCATTTTTATTAATGGCCGTTA TCTACACTAAGCTATACTGCAACTTGGAAAAAGAGGACCTCTCAGAAAACTCACAATCTAGCATGATTAA GCATGTCGCTTGGCTAATCTTCACCAATTGCATCTTTTTCTGCCCTGTGGCGTTTTTTTCATTTGCACCA TTGATCACTGCAATCTCTATCAGCCCCGAAATAATGAAGTCTGTTACTCTGATATTTTTTCCATTGCCTG CTTGCCTGAATCCAGTCCTGTATGTTTTCTTCAACCCAAAGTTTAAAGAAGACTGGAAGTTACTGAAGCG ACGTGTTACCAAGAAAAGTGGATCAGTTTCAGTTTCCATCAGTAGCCAAGGTGGTTGTCTGGAACAGGAT TTCTACTACGACTGTGGCATGTACTCACATTTGCAGGGCAACCTGACTGTTTGCGACTGCTGCGAATCGT TTCTTTTAACAAAGCCAGTATCATGCAAACACTTGATAAAATCACACAGCTGTCCTGCATTGGCAGTGGC TTCTTGCCAAAGACCTGAGGGCTACTGGTCCGACTGTGGCACACAGTCGGCCCACTCTGATTATGCAGAT GAAGAAGATTCCTTTGTCTCAGACAGTTCTGACCAGGTGCAGGCCTGTGGACGAGCCTGCTTCTACCAGA GTAGAGGATTCCCTTTGGTGCGCTATGCTTACAATCTACCAAGAGTTAAAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221345 representing NM_018490
Red=Cloning site Green=Tags(s) MPGPLGLLCFLALGLLGSAGPSGAAPPLCAAPCSCDGDRRVDCSGKGLTAVPEGLSAFTQALDISMNNIT QLPEDAFKNFPFLEELQLAGNDLSFIHPKALSGLKELKVLTLQNNQLKTVPSEAIRGLSALQSLRLDANH ITSVPEDSFEGLVQLRHLWLDDNSLTEVPVHPLSNLPTLQALTLALNKISSIPDFAFTNLSSLVVLHLHN NKIRSLSQHCFDGLDNLETLDLNYNNLGEFPQAIKALPSLKELGFHSNSISVIPDGAFDGNPLLRTIHLY DNPLSFVGNSAFHNLSDLHSLVIRGASMVQQFPNLTGTVHLESLTLTGTKISSIPNNLCQEQKMLRTLDL SYNNIRDLPSFNGCHALEEISLQRNQIYQIKEGTFQGLISLRILDLSRNLIHEIHSRAFATLGPITNLDV SFNELTSFPTEGLNGLNQLKLVGNFKLKEALAAKDFVNLRSLSVPYAYQCCAFWGCDSYANLNTEDNSLQ DHSVAQEKGTADAANVTSTLENEEHSQIIIHCTPSTGAFKPCEYLLGSWMIRLTVWFIFLVALFFNLLVI LTTFASCTSLPSSKLFIGLISVSNLFMGIYTGILTFLDAVSWGRFAEFGIWWETGSGCKVAGFLAVFSSE SAIFLLMLATVERSLSAKDIMKNGKSNHLKQFRVAALLAFLGATVAGCFPLFHRGEYSASPLCLPFPTGE TPSLGFTVTLVLLNSLAFLLMAVIYTKLYCNLEKEDLSENSQSSMIKHVAWLIFTNCIFFCPVAFFSFAP LITAISISPEIMKSVTLIFFPLPACLNPVLYVFFNPKFKEDWKLLKRRVTKKSGSVSVSISSQGGCLEQD FYYDCGMYSHLQGNLTVCDCCESFLLTKPVSCKHLIKSHSCPALAVASCQRPEGYWSDCGTQSAHSDYAD EEDSFVSDSSDQVQACGRACFYQSRGFPLVRYAYNLPRVKD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018490 |
| ORF Size | 2853 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018490.4 |
| RefSeq Size | 5211 bp |
| RefSeq ORF | 2856 bp |
| Locus ID | 55366 |
| UniProt ID | Q9BXB1 |
| Domains | 7tm_1, LRRNT, LRR, LRR_TYP, LRR_BAC, LRR_PS |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| MW | 104.3 kDa |
| Gene Summary | The protein encoded by this gene is a G-protein coupled receptor that binds R-spondins and activates the Wnt signaling pathway. This Wnt signaling pathway activation is necessary for proper development of many organs of the body. [provided by RefSeq, Oct 2016] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Establishment of a tagged variant of Lgr4 receptor suitable for functional and expression studies in the mouse
,Kriz, V;Krausova, M;Buresova, P;Dobes, J;Hrckulak, D;Babosova, O;Svec, J;Korinek, V;,
Transgenic Res.
,PubMed ID 28634819
[GPR48]
|
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221345L1 | Lenti ORF clone of Human leucine-rich repeat containing G protein-coupled receptor 4 (LGR4), Myc-DDK-tagged |
CNY 12,848.00 |
|
| RC221345L2 | Lenti ORF clone of Human leucine-rich repeat containing G protein-coupled receptor 4 (LGR4), mGFP tagged |
CNY 9,030.00 |
|
| RC221345L3 | Lenti ORF clone of Human leucine-rich repeat containing G protein-coupled receptor 4 (LGR4), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC221345L4 | Lenti ORF clone of Human leucine-rich repeat containing G protein-coupled receptor 4 (LGR4), mGFP tagged |
CNY 9,030.00 |
|
| RG221345 | LGR4 (tGFP-tagged) - Human leucine-rich repeat containing G protein-coupled receptor 4 (LGR4) |
CNY 12,048.00 |
|
| SC111332 | LGR4 (untagged)-Human leucine-rich repeat containing G protein-coupled receptor 4 (LGR4) |
CNY 10,456.00 |
