MARCH I (MARCH1) (NM_017923) Human Tagged ORF Clone
CAT#: RC222000
- TrueORF®
MARCH1 (Myc-DDK-tagged)-Human membrane-associated ring finger (C3HC4) 1 (MARCH1), transcript variant 2
ORF Plasmid: tGFP
"NM_017923" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MARCH-I; MARCH1; RNF171 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC222000 representing NM_017923
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCAGCAGCCACGTTTGTTGTAATTTTTTAAATATGTGGAAAAAAAGCAAGATATCAACCATGTATT ACCTTAACCAAGATGCCAAATTATCTAACTTGTTTCTCCAGGCAAGCAGCCCAACAACAGGGACAGCTCC CAGGAGCCAGTCAAGGTTGTCTGTCTGTCCATCCACTCAGGACATCTGCAGAATCTGTCACTGCGAAGGG GATGAAGAGAGCCCCCTCATCACACCCTGTCGCTGCACTGGGACACTGCGCTTTGTCCACCAGTCCTGCC TCCACCAGTGGATAAAGAGCTCAGATACACGCTGCTGTGAGCTCTGCAAGTATGACTTCATAATGGAGAC CAAGCTCAAACCCCTCCGGAAGTGGGAGAAACTACAGATGACCACAAGTGAAAGGAGGAAAATATTCTGC TCTGTCACATTCCACGTAATCGCGATCACCTGTGTGGTTTGGTCTTTGTATGTATTGATAGACCGGACAG CGGAGGAAATCAAGCAAGGCAATGACAATGGTGTCCTTGAATGGCCATTTTGGACAAAACTGGTTGTGGT AGCCATTGGCTTCACAGGAGGTCTTGTCTTCATGTACGTACAGTGTAAAGTCTATGTTCAGTTGTGGCGC AGGCTGAAGGCCTACAACCGTGTGATCTTTGTACAAAATTGCCCAGACACTGCCAAAAAACTGGAGAAGA ACTTCTCATGTAATGTAAACACAGACATCAAAGATGCTGTGGTAGTGCCTGTACCACAAACAGGTGCAAA TTCACTGCCATCTGCAGAGGGTGGCCCCCCTGAAGTTGTATCAGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222000 representing NM_017923
Red=Cloning site Green=Tags(s) MTSSHVCCNFLNMWKKSKISTMYYLNQDAKLSNLFLQASSPTTGTAPRSQSRLSVCPSTQDICRICHCEG DEESPLITPCRCTGTLRFVHQSCLHQWIKSSDTRCCELCKYDFIMETKLKPLRKWEKLQMTTSERRKIFC SVTFHVIAITCVVWSLYVLIDRTAEEIKQGNDNGVLEWPFWTKLVVVAIGFTGGLVFMYVQCKVYVQLWR RLKAYNRVIFVQNCPDTAKKLEKNFSCNVNTDIKDAVVVPVPQTGANSLPSAEGGPPEVVSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_017923 |
ORF Size | 816 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_017923.4 |
RefSeq Size | 1790 bp |
RefSeq ORF | 819 bp |
Locus ID | 55016 |
UniProt ID | Q8TCQ1 |
Domains | RING |
Protein Families | Druggable Genome, Transmembrane |
MW | 30.7 kDa |
Gene Summary | MARCH1 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH1 downregulates the surface expression of major histocompatibility complex (MHC) class II molecules (see MIM 142880) and other glycoproteins by directing them to the late endosomal/lysosomal compartment (Bartee et al., 2004 [PubMed 14722266]; Thibodeau et al., 2008 [PubMed 18389477]; De Gassart et al., 2008 [PubMed 18305173]).[supplied by OMIM, Mar 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC222000L3 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 1 (MARCH1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC222000L4 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 1 (MARCH1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG222000 | 41334 (tGFP-tagged) - Human membrane-associated ring finger (C3HC4) 1 (MARCH1), transcript variant 2 |
CNY 4,370.00 |
|
SC312747 | 41334 (untagged)-Human membrane-associated ring finger (C3HC4) 1 (MARCH1), transcript variant 2 |
CNY 6,270.00 |
其它MARCH I产品