POGK (NM_017542) Human Tagged ORF Clone

CAT#: RC222119

POGK (Myc-DDK-tagged)-Human pogo transposable element with KRAB domain (POGK)

ORF Plasmid: DDK tGFP



  "NM_017542" in other vectors (6)

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CNY 4,536.00

CNY 4,560.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


POGK mouse monoclonal antibody,clone OTI6D5
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms BASS2; KRBOX2; LST003
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC222119 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCCACAGCCTACCCTCTCAATTTGAGCCTGAAAGAAGAGGAAGAGGAAGAAGAGATTCAGAGCC
GGGAACTAGAGGACGGCCCGGCAGACATGCAGAAAGTACGAATCTGCTCTGAGGGCGGATGGGTACCGGC
CCTATTTGATGAGGTGGCCATATATTTTTCCGATGAGGAATGGGAAGTTTTGACGGAGCAACAAAAGGCC
CTCTACCGGGAAGTCATGAGGATGAATTATGAAACTGTCCTGTCCCTGGAATTCCCATTCCCTAAGCCAG
ACATGATCACCCGTTTGGAAGGGGAGGAGGAGTCTCAGAATTCTGACGAGTGGCAGCTCCAAGGAGGCAC
CTCTGCAGAAAATGAAGAATCTGACGTAAAGCCTCCAGACTGGCCAAACCCAATGAATGCTACCTCCCAG
TTTCCTCAGCCTCAGCACTTTGACAGCTTTGGCCTCCGTCTGCCTCGGGATATCACAGAGCTGCCCGAGT
GGAGTGAGGGGTACCCCTTCTACATGGCCATGGGCTTCCCAGGGTATGACCTCTCGGCTGATGACATAGC
TGGGAAGTTTCAGTTCAGCCGGGGCATGCGCCGCAGTTACGACGCAGGGTTCAAGCTGATGGTAGTGGAA
TATGCTGAGAGTACCAACAACTGCCAGGCTGCCAAGCAGTTTGGAGTATTGGAAAAAAACGTTCGAGACT
GGCGCAAAGTGAAGCCACAGCTTCAAAACGCCCACGCCATGCGGCGGGCATTCCGAGGCCCCAAGAATGG
GAGGTTTGCTCTGGTGGACCAGCGTGTGGCCGAATATGTCAGATACATGCAGGCCAAAGGGGACCCCATC
ACCCGGGAGGCGATGCAGCTGAAAGCTCTCGAAATCGCCCAGGAAATGAACATTCCAGAGAAAGGGTTCA
AGGCAAGCTTGGGTTGGTGTCGAAGAATGATGAGAAGGTATGACCTGTCTCTGAGGCATAAAGTGCCCGT
GCCCCAGCACCTGCCGGAAGACCTGACTGAGAAACTCGTCACTTACCAGCGCAGTGTCCTGGCTCTGCGC
AGGGCGCATGACTATGAGGTAGCTCAGATGGGGAATGCAGATGAGACGCCCATTTGTTTAGAGGTGCCAT
CACGGGTAACTGTTGATAACCAGGGCGAAAAGCCTGTCTTGGTCAAGACACCAGGCAGGGAAAAACTGAA
AATCACAGCAATGCTTGGTGTCTTGGCTGATGGGAGGAAGTTACCACCGTACATCATTTTGAGGGGAACA
TATATCCCCCCGGGGAAGTTTCCCAGTGGGATGGAAATTCGCTGCCACCGGTATGGGTGGATGACTGAAG
ACTTGATGCAGGACTGGTTGGAAGTGGTGTGGAGACGGAGGACAGGAGCAGTGCCCAAGCAGCGAGGGAT
GCTGATCTTGAATGGCTTCCGGGGCCATGCCACAGATTCCGTGAAGAACTCCATGGAAAGCATGAACACT
GACATGGTGATCATCCCAGGGGGTCTGACCTCACAGCTTCAGGTGCTGGATGTCGTGGTCTACAAGCCAC
TGAATGACAGTGTGCGGGCCCAGTACTCCAACTGGCTTCTGGCTGGGAACCTGGCGCTGAGCCCAACCGG
GAATGCTAAGAAGCCACCCCTGGGCCTCTTTCTGGAGTGGGTCATGGTCGCGTGGAATAGCATCTCAAGT
GAGTCCATCGTCCAAGGGTTCAAGAAGTGCCATATCTCCAGCAACTTGGAGGAGGAAGACGATGTCCTGT
GGGAAATCGAGAGTGAGTTGCCAGGAGGAGGAGAACCACCAAAAGATTGTGACACCGAAAGCATGGCTGA
GAGCAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC222119 protein sequence
Red=Cloning site Green=Tags(s)

MESTAYPLNLSLKEEEEEEEIQSRELEDGPADMQKVRICSEGGWVPALFDEVAIYFSDEEWEVLTEQQKA
LYREVMRMNYETVLSLEFPFPKPDMITRLEGEEESQNSDEWQLQGGTSAENEESDVKPPDWPNPMNATSQ
FPQPQHFDSFGLRLPRDITELPEWSEGYPFYMAMGFPGYDLSADDIAGKFQFSRGMRRSYDAGFKLMVVE
YAESTNNCQAAKQFGVLEKNVRDWRKVKPQLQNAHAMRRAFRGPKNGRFALVDQRVAEYVRYMQAKGDPI
TREAMQLKALEIAQEMNIPEKGFKASLGWCRRMMRRYDLSLRHKVPVPQHLPEDLTEKLVTYQRSVLALR
RAHDYEVAQMGNADETPICLEVPSRVTVDNQGEKPVLVKTPGREKLKITAMLGVLADGRKLPPYIILRGT
YIPPGKFPSGMEIRCHRYGWMTEDLMQDWLEVVWRRRTGAVPKQRGMLILNGFRGHATDSVKNSMESMNT
DMVIIPGGLTSQLQVLDVVVYKPLNDSVRAQYSNWLLAGNLALSPTGNAKKPPLGLFLEWVMVAWNSISS
ESIVQGFKKCHISSNLEEEDDVLWEIESELPGGGEPPKDCDTESMAESN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_017542
ORF Size 1827 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_017542.5
RefSeq Size 3852 bp
RefSeq ORF 1830 bp
Locus ID 57645
UniProt ID Q9P215
Domains KRAB, DDE, CENPB
Protein Families Transcription Factors
MW 69.4 kDa
Gene Summary The exact function of the protein encoded by this gene is not known. However, this gene product contains a KRAB domain (which is involved in protein-protein interactions) at the N-terminus, and a transposase domain at the C-terminus, suggesting that it may belong to the family of DNA-mediated transposons in human. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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