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ISM1 (NM_080826) Human Tagged ORF Clone
CAT＃: RC222172
ISM1 (Myc-DDK-tagged)-Human isthmin 1 homolog (zebrafish) (ISM1)
ORF Plasmid: tGFP
"NM_080826" in other vectors (4)
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CNY 3,656.00
CNY 4,090.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | bA149I18.1; C20orf82; dJ1077I2.1; ISM; Isthmin |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222172 representing NM_080826
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCGCCTGGCGGCCGAGCTGCTGCTGCTGCTGGGGCTGCTGCTGCTCACGCTGCACATCACCGTGC TGCGCGGCTCGGGAGCCGCCGACGGGCCCGACGCGGCCGCGGGCAACGCCAGCCAAGCCCAGCTGCAGAA TAACCTCAACGTGGGAAGTGACACCACATCAGAAACCAGCTTTTCTCTCTCCAAAGAAGCACCAAGGGAG CATCTGGACCACCAGGCTGCACACCAACCCTTCCCCAGACCGCGATTCCGACAAGAGACGGGGCACCCTT CATTGCAAAGAGATTTCCCCAGATCCTTTCTCCTTGATCTACCAAACTTTCCAGATCTTTCCAAAGCTGA TATCAATGGGCAGAATCCAAATATCCAGGTCACCATAGAGGTGGTCGACGGTCCTGACTCTGAAGCAGAT AAAGATCAGCATCCGGAGAATAAGCCCAGCTGGTCAGTCCCATCCCCCGACTGGCGGGCCTGGTGGCAGA GGTCCCTGTCCTTGGCCAGGGCAAACAGCGGGGACCAGGACTACAAGTACGACAGTACCTCAGACGACAG CAACTTCCTCAACCCCCCCAGGGGGTGGGACCATACAGCCCCAGGCCACCGGACTTTTGAAACCAAAGAT CAGCCAGAATATGATTCCACAGATGGCGAGGGTGACTGGAGTCTCTGGTCTGTCTGCAGCGTCACCTGCG GGAACGGCAACCAGAAACGGACCCGGTCTTGTGGCTACGCGTGCACTGCAACAGAATCGAGGACCTGTGA CCGTCCAAACTGCCCAGGAATTGAAGACACTTTTAGGACAGCTGCCACCGAAGTGAGTCTGCTTGCGGGA AGCGAGGAGTTTAATGCCACCAAACTGTTTGAAGTTGACACAGACAGCTGTGAGCGCTGGATGAGCTGCA AAAGCGAGTTCTTAAAGAAGTACATGCACAAGGTGATGAATGACCTGCCCAGCTGCCCCTGCTCCTACCC CACTGAGGTGGCCTACAGCACGGCCGACATCTTCGACCGCATCAAGCGCAAGGACTTCCGCTGGAAGGAC GCCAGCGGGCCCAAGGAGAAGCTGGAGATCTACAAGCCCACTGCCCGGTACTGCATCCGCTCCATGCTGT CCCTGGAGAGCACCACGCTGGCGGCACAGCACTGCTGCTACGGCGACAACATGCAGCTCATCACCAGGGG CAAGGGGGCGGGCACGCCCAACCTCATCAGCACCGAGTTCTCCGCGGAGCTCCACTACAAGGTGGACGTC CTGCCCTGGATTATCTGCAAGGGTGACTGGAGCAGGTATAACGAGGCCCGGCCTCCCAACAACGGACAGA AGTGCACAGAGAGCCCCTCGGACGAGGACTACATCAAGCAGTTCCAAGAGGCCAGGGAATAT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC222172 representing NM_080826
Red=Cloning site Green=Tags(s) MVRLAAELLLLLGLLLLTLHITVLRGSGAADGPDAAAGNASQAQLQNNLNVGSDTTSETSFSLSKEAPRE HLDHQAAHQPFPRPRFRQETGHPSLQRDFPRSFLLDLPNFPDLSKADINGQNPNIQVTIEVVDGPDSEAD KDQHPENKPSWSVPSPDWRAWWQRSLSLARANSGDQDYKYDSTSDDSNFLNPPRGWDHTAPGHRTFETKD QPEYDSTDGEGDWSLWSVCSVTCGNGNQKRTRSCGYACTATESRTCDRPNCPGIEDTFRTAATEVSLLAG SEEFNATKLFEVDTDSCERWMSCKSEFLKKYMHKVMNDLPSCPCSYPTEVAYSTADIFDRIKRKDFRWKD ASGPKEKLEIYKPTARYCIRSMLSLESTTLAAQHCCYGDNMQLITRGKGAGTPNLISTEFSAELHYKVDV LPWIICKGDWSRYNEARPPNNGQKCTESPSDEDYIKQFQEAREY SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_080826 |
| ORF Size | 1392 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_080826.2 |
| RefSeq Size | 2610 bp |
| RefSeq ORF | 1395 bp |
| Locus ID | 140862 |
| UniProt ID | B1AKI9 |
| MW | 51.9 kDa |
| Gene Summary | Acts as an angiogenesis inhibitor.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222172L3 | Lenti ORF clone of Human isthmin 1 homolog (zebrafish) (ISM1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC222172L4 | Lenti ORF clone of Human isthmin 1 homolog (zebrafish) (ISM1), mGFP tagged |
CNY 5,990.00 |
|
| RG222172 | ISM1 (tGFP-tagged) - Human isthmin 1 homolog (zebrafish) (ISM1) |
CNY 4,470.00 |
|
| SC316757 | ISM1 (untagged)-Human isthmin 1 homolog (zebrafish) (ISM1) |
CNY 7,410.00 |
