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Cofilin 2 (CFL2) (NM_138638) Human Tagged ORF Clone
CAT＃: RC222215
CFL2 (Myc-DDK-tagged)-Human cofilin 2 (muscle) (CFL2), transcript variant 2
ORF Plasmid: tGFP
"NM_138638" in other vectors (4)
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CNY 1,200.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NEM7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222215 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCTGGAGTTACAGTGAATGATGAAGTCATCAAAGTTTTTAATGATATGAAAGTAAGGAAATCTT CTACACAAGAGGAGATCAAAAAGAGAAAGAAAGCAGTTCTCTTCTGTTTAAGCGATGACAAAAGACAAAT AATTGTAGAGGAAGCAAAGCAGATCTTGGTGGGTGACATTGGTGATACTGTAGAGGACCCCTACACATCT TTTGTGAAGTTGCTACCTCTGAATGATTGCCGATATGCTTTGTACGATGCCACATACGAAACAAAAGAGT CTAAGAAAGAAGACCTAGTATTTATATTCTGGGCTCCTGAAAGTGCACCTTTAAAAAGCAAGATGATTTA TGCTAGCTCTAAAGATGCCATTAAAAAGAAATTTACAGGTATTAAACATGAGTGGCAAGTAAATGGCTTG GATGATATTAAGGACCGTTCGACACTTGGAGAGAAATTGGGAGGCAATGTAGTAGTTTCACTTGAAGGAA AACCATTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222215 protein sequence
Red=Cloning site Green=Tags(s) MASGVTVNDEVIKVFNDMKVRKSSTQEEIKKRKKAVLFCLSDDKRQIIVEEAKQILVGDIGDTVEDPYTS FVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKFTGIKHEWQVNGL DDIKDRSTLGEKLGGNVVVSLEGKPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138638 |
| ORF Size | 498 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_138638.5 |
| RefSeq Size | 3267 bp |
| RefSeq ORF | 501 bp |
| Locus ID | 1073 |
| UniProt ID | Q9Y281 |
| Protein Families | Druggable Genome |
| Protein Pathways | Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton |
| MW | 18.7 kDa |
| Gene Summary | This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222215L3 | Lenti ORF clone of Human cofilin 2 (muscle) (CFL2), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222215L4 | Lenti ORF clone of Human cofilin 2 (muscle) (CFL2), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG222215 | CFL2 (tGFP-tagged) - Human cofilin 2 (muscle) (CFL2), transcript variant 2 |
CNY 2,800.00 |
|
| SC107636 | CFL2 (untagged)-Human cofilin 2 (muscle) (CFL2), transcript variant 2 |
CNY 2,400.00 |
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