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CLCN2 (NM_004366) Human Tagged ORF Clone
CAT＃: RC222621
CLCN2 (Myc-DDK-tagged)-Human chloride channel 2 (CLCN2), transcript variant 1
ORF Plasmid: tGFP
"NM_004366" in other vectors (6)
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CNY 2,400.00
CNY 9,500.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CIC-2; clC-2; CLC2; ECA2; ECA3; EGI3; EGI11; EGMA; EJM6; EJM8; HALD2; LKPAT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222621 representing NM_004366
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCGCGGCGGCGGAGGAAGGGATGGAGCCACGGGCGCTGCAGTACGAGCAGACCCTGATGTATG GCCGGTACACTCAGGACCTTGGGGCCTTTGCCAAAGAGGAAGCTGCTCGGATTCGCCTGGGAGGGCCTGA ACCCTGGAAAGGTCCCCCTTCCTCTCGGGCTGCCCCAGAGCTCTTGGAATATGGACGGAGCCGTTGCGCC CGATGCCGCGTCTGTTCTGTCCGCTGCCACAAGTTCCTAGTATCCAGGGTTGGTGAAGATTGGATCTTCC TGGTCCTGCTGGGGCTTCTCATGGCATTGGTCAGCTGGGTCATAGACTATGCCATTGCTGCCTGTCTGCA AGCCCAGCAGTGGATGTCCCGGGGCTTGAACACCAGCATCTTGCTCCAGTACCTGGCCTGGGTCACCTAC CCTGTTGTCCTCATCACTTTCTCAGCCGGATTCACACAGATCCTGGCCCCTCAGGCTGTCGGCTCTGGCA TCCCTGAGATGAAGACCATCTTGCGGGGAGTGGTGCTGAAAGAATACCTCACACTCAAGACCTTTATAGC TAAGGTCATTGGGCTGACCTGCGCCCTAGGCAGCGGGATGCCGCTTGGCAAAGAGGGCCCTTTTGTGCAT ATCGCAAGCATGTGTGCTGCCCTTCTCAGCAAGTTCCTCTCCCTCTTTGGGGGTATCTATGAGAATGAAT CCCGGAACACAGAGATGCTGGCTGCCGCCTGTGCCGTGGGGGTGGGCTGCTGCTTCGCGGCACCTATTGG AGGCGTCCTCTTCAGCATCGAGGTCACCTCCACCTTCTTTGCAGTGCGGAACTACTGGCGGGGCTTCTTC GCTGCCACCTTCAGTGCCTTCATCTTCCGGGTCTTGGCAGTCTGGAACCGGGATGAAGAGACTATTACAG CCCTCTTCAAAACCCGATTCCGGCTCGACTTCCCCTTTGACCTGCAGGAGCTGCCAGCCTTTGCTGTCAT TGGTATTGCTAGTGGCTTCGGTGGAGCCCTCTTTGTCTACCTGAACCGGAAGATTGTCCAGGTGATGCGG AAGCAGAAAACCATCAATCGCTTCCTCATGAGGAAACGCCTGCTCTTCCCGGCTCTGGTGACCCTGCTCA TCTCCACGCTGACCTTCCCCCCTGGCTTTGGACAGTTCATGGCTGGACAGCTCTCACAGAAAGAGACGCT GGTCACCCTGTTTGACAATCGGACGTGGGTCCGCCAGGGCCTGGTGGAGGAGCTAGAACCACCCAGCACC TCACAGGCCTGGAACCCACCACGTGCCAACGTCTTCCTCACCCTGGTCATCTTCATTCTCATGAAGTTCT GGATGTCTGCACTGGCCACCACCATCCCAGTTCCCTGTGGGGCCTTCATGCCTGTCTTTGTCATTGGAGC AGCATTTGGGCGTCTGGTGGGTGAAAGCATGGCTGCCTGGTTCCCAGATGGAATTCATACGGACAGCAGC ACCTACCGGATTGTGCCTGGGGGCTACGCTGTGGTCGCTGCGCTGGCAGGAGCGGTGACACACACAGTGT CCACGGCTGTGATCGTGTTCGAGCTCACAGGCCAGATTGCCCACATCCTGCCTGTCATGATCGCCGTCAT CCTGGCCAACGCTGTCGCCCAGAGTCTGCAGCCCTCCCTCTATGACAGCATCATCCGAATCAAGAAACTG CCCTACCTGCCTGAGCTCGGCTGGGGCCGCCACCAGCAGTACCGGGTGCGTGTGGAGGACATCATGGTGC GGGATGTTCCCCATGTGGCCCTCAGCTGCACCTTCCGGGACCTGCGTTTGGCACTGCACAGGACCAAGGG CCGAATGCTGGCCCTAGTGGAGTCCCCTGAGTCCATGATTCTGCTGGGCTCCATCGAGCGTTCACAGGTG GTGGCATTGTTGGGGGCCCAGCTGAGCCCAGCCCGCCGGCGGCAGCACATGCAGGAGCGCAGAGCCACCC AGACCTCTCCACTATCTGATCAGGAGGGTCCCCCTAGCCCTGAGGCTTCTGTCTGCTTCCAGGTGAACAC AGAAGACTCAGCCTTCCCAGCAGCCCGGGGGGAGACCCACAAGCCCCTAAAGCCTGCACTCAAGAGGGGG CCCAGTGTCACCAGGAACCTCGGAGAGAGTCCCACAGGGAGCGCAGAGTCGGCAGGCATCGCCCTCCGGA GCCTCTTCTGTGGCAGTCCACCCCCTGAGGCTGCTTCGGAGAAGTTGGAATCCTGTGAGAAGCGCAAGCT GAAGCGTGTCCGAATCTCCCTGGCAAGTGACGCGGACCTGGAAGGCGAGATGAGCCCTGAAGAGATTCTG GAGTGGGAGGAGCAGCAACTAGATGAACCTGTCAACTTCAGTGACTGCAAAATTGATCCTGCTCCCTTCC AGCTGGTGGAGCGGACCTCTTTGCACAAGACTCACACTATCTTCTCACTGCTGGGAGTGGACCATGCTTA TGTCACCAGTATTGGCAGACTCATTGGAATCGTTACTCTAAAGGAGCTCCGGAAGGCCATCGAGGGCTCT GTCACAGCACAGGGTGTGAAAGTCCGGCCGCCCCTCGCCAGCTTCCGAGACAGTGCCACCAGCAGCAGTG ACACGGAGACCACTGAGGTGCATGCACTCTGGGGGCCCCACTCCCGTCATGGCCTCCCCCGGGAGGGCAG CCCTTCCGACAGCGACGACAAATGCCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222621 representing NM_004366
Red=Cloning site Green=Tags(s) MAAAAAEEGMEPRALQYEQTLMYGRYTQDLGAFAKEEAARIRLGGPEPWKGPPSSRAAPELLEYGRSRCA RCRVCSVRCHKFLVSRVGEDWIFLVLLGLLMALVSWVIDYAIAACLQAQQWMSRGLNTSILLQYLAWVTY PVVLITFSAGFTQILAPQAVGSGIPEMKTILRGVVLKEYLTLKTFIAKVIGLTCALGSGMPLGKEGPFVH IASMCAALLSKFLSLFGGIYENESRNTEMLAAACAVGVGCCFAAPIGGVLFSIEVTSTFFAVRNYWRGFF AATFSAFIFRVLAVWNRDEETITALFKTRFRLDFPFDLQELPAFAVIGIASGFGGALFVYLNRKIVQVMR KQKTINRFLMRKRLLFPALVTLLISTLTFPPGFGQFMAGQLSQKETLVTLFDNRTWVRQGLVEELEPPST SQAWNPPRANVFLTLVIFILMKFWMSALATTIPVPCGAFMPVFVIGAAFGRLVGESMAAWFPDGIHTDSS TYRIVPGGYAVVAALAGAVTHTVSTAVIVFELTGQIAHILPVMIAVILANAVAQSLQPSLYDSIIRIKKL PYLPELGWGRHQQYRVRVEDIMVRDVPHVALSCTFRDLRLALHRTKGRMLALVESPESMILLGSIERSQV VALLGAQLSPARRRQHMQERRATQTSPLSDQEGPPSPEASVCFQVNTEDSAFPAARGETHKPLKPALKRG PSVTRNLGESPTGSAESAGIALRSLFCGSPPPEAASEKLESCEKRKLKRVRISLASDADLEGEMSPEEIL EWEEQQLDEPVNFSDCKIDPAPFQLVERTSLHKTHTIFSLLGVDHAYVTSIGRLIGIVTLKELRKAIEGS VTAQGVKVRPPLASFRDSATSSSDTETTEVHALWGPHSRHGLPREGSPSDSDDKCQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004366 |
| ORF Size | 2688 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004366.6 |
| RefSeq Size | 2697 bp |
| RefSeq ORF | 2697 bp |
| Locus ID | 1181 |
| UniProt ID | P51788 |
| Domains | CBS, voltage_CLC |
| Protein Families | Druggable Genome, Ion Channels: Other, Transmembrane |
| MW | 98.4 kDa |
| Gene Summary | This gene encodes a voltage-gated chloride channel. The encoded protein is a transmembrane protein that maintains chloride ion homeostasis in various cells. Defects in this gene may be a cause of certain epilepsies. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222621L1 | Lenti ORF clone of Human chloride channel 2 (CLCN2), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC222621L2 | Lenti ORF clone of Human chloride channel 2 (CLCN2), transcript variant 1, mGFP tagged |
CNY 11,400.00 |
|
| RC222621L3 | Lenti ORF clone of Human chloride channel 2 (CLCN2), transcript variant 1, Myc-DDK-tagged |
CNY 11,400.00 |
|
| RC222621L4 | Lenti ORF clone of Human chloride channel 2 (CLCN2), transcript variant 1, mGFP tagged |
CNY 11,400.00 |
|
| RG222621 | CLCN2 (tGFP-tagged) - Human chloride channel 2 (CLCN2), transcript variant 1 |
CNY 4,000.00 |
|
| SC117417 | CLCN2 (untagged)-Human chloride channel 2 (CLCN2), transcript variant 1 |
CNY 6,584.00 |
