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RBM15B (NM_013286) Human Tagged ORF Clone
CAT＃: RC222622
RBM15B (Myc-DDK-tagged)-Human RNA binding motif protein 15B (RBM15B)
ORF Plasmid: tGFP
"NM_013286" in other vectors (5)
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CNY 6,520.00
CNY 9,410.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HsOTT3; HUMAGCGB; OTT3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222622 representing NM_013286
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCGGCAGAGCGAGCGAGACTCTAGCCCGAGCGGGCGCGGCTCGTCATCGTCCGCCAAGCGTCCGC GGGAGCGCGAACGGGAGGCGGAGGCGGGCGGGCGGCGGGCGGCGCACAAGGCCTCTGGCGGCGCCAAGCA CCCGGTTCCAGCGCGGGCCCGCGACAAACCCCGCGGCAGCGGAAGCGGCGGGGGCGGGCATCGCGACGGC CGCGGCACCGGGGACGCGAATCACCGCGCGAGTAGCGGGCGCTCCTCGGGCTCCGGCGCTGGCGGCGGGG GACGCGGCGGCAAGGCCTCGGGGGACCCGGGCGCCTCCGGCATGTCGCCCCGCGCGTCTCCTCTGCCGCC GCCTCCGCCACCGCCTGGGGCCGAGCCCGCGTGTCCCGGCTCATCCGCGGCCGCGCCTGAGTACAAGACG TTGCTCATCAGCAGCTTGAGCCCCGCGCTGCCCGCCGAGCACCTCGAGGACCGGCTCTTCCACCAGTTCA AGCGCTTCGGCGAGATCAGCCTCCGCCTGTCGCACACGCCTGAGCTGGGCCGTGTGGCCTACGTGAATTT CCGGCACCCACAGGACGCACGCGAGGCCCGCCAGCACGCCCTGGCCCGGCAGCTGCTGCTCTACGACCGC CCGCTCAAGGTAGAGCCCGTGTACCTGCGTGGCGGCGGCGGGAGCAGTCGGCGAAGTAGCAGCAGCAGCG CCGCCGCTTCCACGCCTCCCCCAGGGCCGCCCGCGCCCGCCGACCCGCTCGGCTACCTCCCGCTACACGG AGGCTACCAGTACAAGCAGCGCTCGCTGTCCCCCGTCGCTGCCCCGCCCCTGCGGGAGCCCCGTGCCCGT CACGCCGCCGCAGCCTTCGCCCTGGATGCCGCTGCTGCCGCCGCCGTGGGACTGTCCCGGGAGCGGGCCC TGGACTACTACGGGCTGTACGACGACCGTGGGCGCCCCTATGGCTACCCAGCTGTGTGTGAGGAGGACCT GATGCCCGAGGATGACCAGCGGGCCACGCGCAACCTCTTCATTGGTAACCTGGACCACAGCGTATCTGAG GTGGAGCTGCGAAGGGCCTTCGAGAAATATGGCATCATCGAGGAGGTGGTCATCAAGAGGCCTGCCCGTG GCCAGGGCGGTGCCTATGCCTTCCTCAAGTTCCAGAACCTGGACATGGCCCATAGGGCTAAGGTGGCCAT GTCGGGCCGAGTGATTGGTCGCAACCCCATTAAGATAGGCTATGGCAAGGCCAACCCCACCACTCGTCTC TGGGTGGGTGGCCTGGGACCTAACACGTCACTGGCGGCTCTGGCCCGAGAGTTTGACCGCTTTGGGAGCA TTCGGACCATTGATCACGTCAAAGGAGATAGCTTTGCCTATATTCAGTACGAGAGCTTGGACGCAGCCCA GGCCGCCTGTGCTAAAATGAGGGGTTTTCCCTTGGGTGGACCAGACCGCAGGCTCCGCGTGGATTTTGCC AAAGCAGAGGAGACTCGGTACCCCCAGCAGTACCAGCCCTCGCCACTCCCTGTGCATTATGAGCTGCTCA CAGATGGATACACCCGGCACCGCAACCTGGACGCCGACCTGGTGCGGGACAGGACGCCCCCACACCTTCT GTACTCAGACCGAGACCGGACTTTTTTGGAAGGGGACTGGACCAGCCCCAGTAAAAGCTCTGACCGCCGA AACAGCCTTGAGGGCTACAGTCGCTCAGTGCGCAGCCGGAGTGGTGAGCGTTGGGGGGCAGATGGAGACC GTGGTTTGCCCAAGCCCTGGGAAGAGAGGCGGAAACGGAGAAGCCTTTCCAGTGACCGTGGGAGGACAAC CCATTCACCATATGAGGAACGGAGTAGGACCAAGGGCAGTGGGCAGCAGTCAGAGCGGGGCTCCGACCGC ACCCCTGAGCGCAGCCGCAAGGAGAACCACTCCAGTGAAGGGACCAAGGAGTCCAGCAGCAACTCCCTCA GCAACAGCAGACATGGGGCTGAGGAACGGGGCCACCACCACCACCACCACGAGGCTGCAGACTCTTCCCA CGGGAAGAAGGCAAGAGACAGCGAGCGCAATCACCGGACCACAGAGGCCGAGCCCAAGCCTCTGGAAGAG CCAAAACACGAGACCAAAAAGCTGAAGAATCTTTCAGAGTACGCTCAGACACTACAGCTGGGTTGGAATG GGCTTCTGGTGTTGAAAAACAGCTGCTTCCCCACGTCTATGCATATCCTAGAGGGGGACCAGGGGGTGAT CAGCAGTCTCCTCAAAGACCACACTTCTGGGAGCAAGCTGACCCAGCTGAAGATCGCCCAGCGCCTTCGA CTGGACCAGCCCAAGCTTGACGAGGTCACACGACGCATCAAGCAGGGGAGCCCCAACGGCTATGCGGTCC TCTTAGCCACCCAGGCAACCCCCAGTGGGCTTGGCACTGAGGGGATGCCCACAGTAGAGCCCGGTCTGCA GAGGCGGCTTCTCAGGAACCTGGTCTCCTACTTGAAACAGAAGCAGGCCGCAGGGGTGATCAGCTTGCCA GTGGGGGGGTCCAAGGGCAGAGACGGCACAGGCATGCTCTACGCCTTCCCACCCTGCGACTTTTCCCAGC AGTACCTCCAGTCAGCACTAAGGACATTGGGCAAGCTAGAAGAAGAACACATGGTGATAGTCATCGTCAG AGACACTGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222622 representing NM_013286
Red=Cloning site Green=Tags(s) MKRQSERDSSPSGRGSSSSAKRPREREREAEAGGRRAAHKASGGAKHPVPARARDKPRGSGSGGGGHRDG RGTGDANHRASSGRSSGSGAGGGGRGGKASGDPGASGMSPRASPLPPPPPPPGAEPACPGSSAAAPEYKT LLISSLSPALPAEHLEDRLFHQFKRFGEISLRLSHTPELGRVAYVNFRHPQDAREARQHALARQLLLYDR PLKVEPVYLRGGGGSSRRSSSSSAAASTPPPGPPAPADPLGYLPLHGGYQYKQRSLSPVAAPPLREPRAR HAAAAFALDAAAAAAVGLSRERALDYYGLYDDRGRPYGYPAVCEEDLMPEDDQRATRNLFIGNLDHSVSE VELRRAFEKYGIIEEVVIKRPARGQGGAYAFLKFQNLDMAHRAKVAMSGRVIGRNPIKIGYGKANPTTRL WVGGLGPNTSLAALAREFDRFGSIRTIDHVKGDSFAYIQYESLDAAQAACAKMRGFPLGGPDRRLRVDFA KAEETRYPQQYQPSPLPVHYELLTDGYTRHRNLDADLVRDRTPPHLLYSDRDRTFLEGDWTSPSKSSDRR NSLEGYSRSVRSRSGERWGADGDRGLPKPWEERRKRRSLSSDRGRTTHSPYEERSRTKGSGQQSERGSDR TPERSRKENHSSEGTKESSSNSLSNSRHGAEERGHHHHHHEAADSSHGKKARDSERNHRTTEAEPKPLEE PKHETKKLKNLSEYAQTLQLGWNGLLVLKNSCFPTSMHILEGDQGVISSLLKDHTSGSKLTQLKIAQRLR LDQPKLDEVTRRIKQGSPNGYAVLLATQATPSGLGTEGMPTVEPGLQRRLLRNLVSYLKQKQAAGVISLP VGGSKGRDGTGMLYAFPPCDFSQQYLQSALRTLGKLEEEHMVIVIVRDTA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013286 |
| ORF Size | 2670 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013286.5 |
| RefSeq Size | 6620 bp |
| RefSeq ORF | 2673 bp |
| Locus ID | 29890 |
| UniProt ID | Q8NDT2 |
| Protein Families | Transcription Factors |
| MW | 97 kDa |
| Gene Summary | Members of the SPEN (Split-end) family of proteins, including RBM15B, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components (Hiriart et al., 2005 [PubMed 16129689]).[supplied by OMIM, Feb 2009] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
hnRNP G/RBMX enhances HPV16 E2 mRNA splicing through a novel splicing enhancer and inhibits production of spliced E7 oncogene mRNAs
,null,
Nucleic Acids Research
,PubMed ID 35357488
[RBM15B]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222622L3 | Lenti ORF clone of Human RNA binding motif protein 15B (RBM15B), Myc-DDK-tagged |
CNY 11,310.00 |
|
| RC222622L4 | Lenti ORF clone of Human RNA binding motif protein 15B (RBM15B), mGFP tagged |
CNY 11,310.00 |
|
| RG222622 | RBM15B (tGFP-tagged) - Human RNA binding motif protein 15B (RBM15B) |
CNY 10,360.00 |
|
| SC115329 | RBM15B (untagged)-Human RNA binding motif protein 15B (RBM15B) |
CNY 2,400.00 |
|
| SC318363 | RBM15B (untagged)-Human RNA binding motif protein 15B (RBM15B) |
CNY 17,100.00 |
