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ZNF148 (NM_021964) Human Tagged ORF Clone
CAT＃: RC222687
ZNF148 (Myc-DDK-tagged)-Human zinc finger protein 148 (ZNF148)
ORF Plasmid: tGFP
"NM_021964" in other vectors (6)
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CNY 5,816.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52; ZBP-89; ZFP148 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222687 representing NM_021964
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACATTGACGACAAACTGGAAGGATTGTTTCTTAAATGTGGCGGCATAGACGAAATGCAGTCTTCCA GGACAATGGTTGTAATGGGTGGAGTGTCTGGCCAGTCTACTGTGTCTGGAGAGCTACAGGATTCAGTACT TCAAGATCGAAGTATGCCTCACCAGGAGATCCTTGCTGCAGATGAAGTGTTACAAGAAAGTGAAATGAGA CAACAGGATATGATATCACATGATGAACTCATGGTCCATGAGGAGACAGTGAAAAATGATGAAGAGCAGA TGGAAACACATGAAAGACTTCCTCAAGGACTACAGTATGCACTTAATGTCCCTATAAGCGTAAAGCAGGA AATTACTTTTACTGATGTATCTGAGCAACTGATGAGAGACAAAAAACAAATCAGAGAGCCAGTAGACTTA CAGAAAAAGAAGAAGCGGAAACAACGTTCTCCCGCAAAAATCCTTACAATAAATGAGGATGGATCACTTG GTTTGAAAACCCCTAAATCTCACGTTTGTGAGCACTGCAATGCTGCCTTTAGAACGAACTATCACTTACA GAGACATGTCTTCATTCATACAGGTGAAAAACCATTTCAATGTAGTCAATGTGACATGCGTTTCATACAG AAGTACCTGCTTCAGAGACATGAGAAGATTCATACTGGTGAAAAACCATTTCGCTGTGATGAATGTGGTA TGAGATTCATACAAAAATATCATATGGAAAGGCATAAGAGAACTCATAGTGGAGAAAAACCTTACCAGTG TGAATACTGTTTACAGTATTTTTCCAGAACAGATCGTGTATTGAAACATAAACGTATGTGCCATGAAAAT CATGACAAAAAACTAAATAGATGTGCCATCAAAGGTGGCCTTCTGACATCTGAGGAAGATTCTGGCTTTT CTACATCACCAAAAGACAACTCACTGCCAAAAAAGAAAAGGCAGAAAACGGAGAAAAAATCATCTGGAAT GGACAAAGAGAGTGCTTTGGACAAATCTGACCTGAAAAAAGACAAAAATGATTACTTGCCTCTTTATTCT TCAAGTACTAAAGTAAAAGATGAGTATATGGTTGCAGAATATGCTGTTGAAATGCCACATTCGTCAGTTG GGGGCTCGCATTTAGAAGATGCGTCAGGAGAAATACACCCACCTAAGTTAGTTCTCAAAAAAATTAATAG TAAGAGAAGTCTGAAACAGCCACTGGAGCAAAATCAAACAATTTCACCTTTATCCACATATGAAGAGAGC AAAGTTTCAAAGTATGCTTTTGAACTTGTGGATAAACAGGCTTTACTGGACTCAGAAGGCAATGCTGACA TTGATCAGGTTGATAATTTGCAGGAGGGGCCCAGTAAACCTGTGCATAGTAGTACTAATTATGATGATGC CATGCAGTTTTTGAAGAAGAAGCGGTATCTTCAAGCAGCAAGTAACAACAGCAGGGAATATGCGCTGAAT GTGGGTACCATAGCTTCTCAGCCTTCTGTAACACAAGCAGCTGTGGCAAGTGTCATTGATGAAAGTACCA CGGCATCCATATTAGAGTCACAGGCACTGAATGTGGAGATTAAGAGTAATCATGACAAAAATGTTATTCC AGATGAGGTACTGCAGACTCTGTTGGATCATTATTCCCACAAAGCTAATGGACAGCATGAGATATCCTTC AGTGTTGCAGATACTGAAGTGACTTCTAGCATATCAATAAATTCTTCAGAAGTACCAGAGGTCACCCCAT CAGAGAATGTTGGATCAAGCTCCCAAGCATCCTCATCAGATAAAGCCAACATGTTGCAGGAATACTCCAA GTTTCTGCAGCAGGCTTTGGACAGAACTAGCCAAAATGATGCCTATTTGAATAGCCCGAGCCTTAACTTT GTGACTGATAACCAGACCCTCCCAAATCAGCCAGCATTCTCTTCCATAGACAAGCAGGTCTATGCCACCA TGCCCATCAATAGCTTTCGATCAGGAATGAATTCTCCACTAAGAACAACTCCAGATAAGTCCCACTTTGG ACTAATAGTTGGTGATTCACAGCACTCATTTCCCTTTTCAGGTGATGAGACAAACCATGCTTCTGCCACA TCAACACAGGACTTTCTGGATCAAGTGACTTCTCAGAAGAAAGCTGAGGCCCAGCCTGTCCACCAAGCTT ACCAAATGAGCTCCTTTGAACAGCCCTTCCGTGCTCCCTATCATGGATCAAGAGCTGGAATAGCTACTCA ATTTAGCACTGCCAATGGACAGGTGAACCTTCGGGGACCAGGGACAAGTGCTGAATTTTCAGAATTTCCC TTGGTGAATGTAAATGATAATAGAGCTGGGATGACATCTTCACCTGATGCCACAACTGGCCAGACTTTTG GC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222687 representing NM_021964
Red=Cloning site Green=Tags(s) MNIDDKLEGLFLKCGGIDEMQSSRTMVVMGGVSGQSTVSGELQDSVLQDRSMPHQEILAADEVLQESEMR QQDMISHDELMVHEETVKNDEEQMETHERLPQGLQYALNVPISVKQEITFTDVSEQLMRDKKQIREPVDL QKKKKRKQRSPAKILTINEDGSLGLKTPKSHVCEHCNAAFRTNYHLQRHVFIHTGEKPFQCSQCDMRFIQ KYLLQRHEKIHTGEKPFRCDECGMRFIQKYHMERHKRTHSGEKPYQCEYCLQYFSRTDRVLKHKRMCHEN HDKKLNRCAIKGGLLTSEEDSGFSTSPKDNSLPKKKRQKTEKKSSGMDKESALDKSDLKKDKNDYLPLYS SSTKVKDEYMVAEYAVEMPHSSVGGSHLEDASGEIHPPKLVLKKINSKRSLKQPLEQNQTISPLSTYEES KVSKYAFELVDKQALLDSEGNADIDQVDNLQEGPSKPVHSSTNYDDAMQFLKKKRYLQAASNNSREYALN VGTIASQPSVTQAAVASVIDESTTASILESQALNVEIKSNHDKNVIPDEVLQTLLDHYSHKANGQHEISF SVADTEVTSSISINSSEVPEVTPSENVGSSSQASSSDKANMLQEYSKFLQQALDRTSQNDAYLNSPSLNF VTDNQTLPNQPAFSSIDKQVYATMPINSFRSGMNSPLRTTPDKSHFGLIVGDSQHSFPFSGDETNHASAT STQDFLDQVTSQKKAEAQPVHQAYQMSSFEQPFRAPYHGSRAGIATQFSTANGQVNLRGPGTSAEFSEFP LVNVNDNRAGMTSSPDATTGQTFG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021964 |
| ORF Size | 2382 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021964.3 |
| RefSeq Size | 3032 bp |
| RefSeq ORF | 2385 bp |
| Locus ID | 7707 |
| UniProt ID | Q9UQR1 |
| Domains | zf-C2H2 |
| Protein Families | Transcription Factors |
| MW | 88.8 kDa |
| Gene Summary | The protein encoded by this gene is a member of the Kruppel family of zinc finger DNA binding proteins. The encoded protein activates transcription of the T-cell receptor and intestinal alkaline phosphatase genes but represses transcription of the ornithine decarboxylase, vimentin, gastrin, stomelysin, and enolase genes. Increased expression of this gene results in decreased patient survival rates from colorectal cancer, while mutations in this gene have been associated with global developmental delay, hypoplastic corpus callosum, and dysmorphic facies. [provided by RefSeq, Feb 2017] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Functional characterization of a multi-cancer risk locus on chr5p15.33 reveals regulation of TERT by ZNF148
,null,
Nature Communications
,PubMed ID 28447668
[ZNF148]
|
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222687L1 | Lenti ORF clone of Human zinc finger protein 148 (ZNF148), Myc-DDK-tagged |
CNY 8,216.00 |
|
| RC222687L2 | Lenti ORF clone of Human zinc finger protein 148 (ZNF148), mGFP tagged |
CNY 5,890.00 |
|
| RC222687L3 | Lenti ORF clone of Human zinc finger protein 148 (ZNF148), Myc-DDK-tagged |
CNY 8,216.00 |
|
| RC222687L4 | Lenti ORF clone of Human zinc finger protein 148 (ZNF148), mGFP tagged |
CNY 8,216.00 |
|
| RG222687 | ZNF148 (tGFP-tagged) - Human zinc finger protein 148 (ZNF148) |
CNY 7,416.00 |
|
| SC112833 | ZNF148 (untagged)-Human zinc finger protein 148 (ZNF148) |
CNY 5,824.00 |
