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SIM1 (NM_005068) Human Tagged ORF Clone
CAT＃: RC222805
SIM1 (Myc-DDK-tagged)-Human single-minded homolog 1 (Drosophila) (SIM1)
ORF Plasmid: tGFP
"NM_005068" in other vectors (4)
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CNY 8,416.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | bHLHe14 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222805 representing NM_005068
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGAAAAGTCCAAAAATGCTGCGCGGACTAGGAGGGAGAAGGAAAACAGTGAATTTTATGAACTGG CTAAATTACTGCCTTTGCCCTCGGCTATCACCTCGCAGCTGGACAAAGCATCCATAATCAGACTCACGAC CAGCTATCTCAAAATGAGAGTGGTGTTCCCAGAAGGGCTCGGCGAGGCGTGGGGCCACTCAAGTCGGACC AGCCCCCTGGACAACGTTGGCCGAGAACTGGGCTCCCATCTGCTCCAGACCCTGGATGGCTTCATCTTCG TGGTAGCCCCAGATGGGAAGATCATGTACATCTCAGAGACAGCCTCAGTCCACTTGGGTCTTTCTCAGGT AGAGCTGACCGGAAACAGCATTTATGAATACATTCACCCGGCAGACCACGACGAGATGACGGCGGTGCTC ACCGCCCATCAACCCTACCACTCTCACTTCGTGCAGGAGTATGAGATCGAGCGCTCCTTCTTCCTGAGGA TGAAGTGCGTCTTGGCCAAGCGTAACGCCGGCCTCACCTGTGGCGGCTACAAGGTCATCCACTGCAGCGG CTACTTGAAGATCCGCCAGTACAGCCTGGACATGTCCCCCTTCGACGGCTGCTACCAAAACGTGGGCCTG GTGGCCGTGGGCCACTCGCTGCCTCCCAGCGCCGTCACGGAGATCAAGCTACACAGCAATATGTTTATGT TCCGCGCCAGCCTGGACATGAAGCTCATCTTTCTGGACTCCAGGGTGGCGGAGCTGACGGGGTACGAACC TCAGGACCTGATTGAGAAGACTCTGTACCACCATGTGCACGGCTGCGACACCTTCCACCTGCGCTGCGCG CACCATTTGCTGCTGGTGAAGGGACAGGTGACCACCAAGTACTACAGGTTCCTGGCGAAACACGGCGGCT GGGTATGGGTGCAGAGCTACGCGACCATCGTGCACAACAGTCGCTCCTCCAGGCCACACTGTATCGTCAG CGTCAACTATGTCCTCACAGACACAGAATACAAAGGGCTGCAGCTCTCCCTGGATCAGATCTCAGCCTCC AAACCAGCCTTCTCCTATACCAGCAGCTCCACCCCCACCATGACTGACAACAGAAAGGGGGCCAAATCCC GGCTCTCCAGCTCAAAGTCAAAATCCAGGACTTCCCCATACCCTCAGTATTCGGGATTTCACACAGAAAG ATCGGAATCTGATCATGACAGCCAGTGGGGCGGAAGTCCCTTGACCGACACGGCCTCTCCGCAGCTTCTG GACCCCGCCGATAGGCCTGGCTCCCAGCACGACGCATCGTGCGCCTACAGACAGTTTTCGGACCGCAGCT CTCTCTGCTATGGCTTTGCGCTTGACCACTCGAGGCTGGTGGAAGAGAGGCATTTCCATACCCAGGCCTG TGAAGGAGGCCGATGTGAGGCAGGCAGGTACTTCCTGGGAACGCCGCAGGCCGGGAGGGAGCCCTGGTGG GGCTCTCGCGCAGCCTTGCCCCTGACAAAGGCCTCCCCAGAAAGCAGAGAAGCCTATGAAAACAGCATGC CTCACATCGCTTCAGTCCACAGGATCCATGGGCGAGGTCATTGGGATGAAGATAGTGTGGTCAGTTCTCC AGACCCTGGGTCGGCCAGTGAATCAGGTGACCGATATCGTACTGAGCAGTATCAAAGTAGCCCACATGAA CCCAGCAAAATTGAAACTCTTATAAGAGCCACTCAGCAAATGATTAAAGAAGAAGAGAACAGATTACAGC TAAGGAAAGCCCCCTCAGACCAACTGGCTTCCATTAATGGGGCTGGGAAAAAACACTCCCTGTGTTTTGC AAACTACCAACAGCCCCCACCAACAGGTGAAGTCTGCCATGGCTCTGCTCTTGCCAACACTTCACCATGT GACCATATCCAGCAGAGAGAGGGAAAAATGTTGAGCCCCCATGAAAATGACTATGACAACAGTCCCACCG CACTATCTCGGATAAGTAGTCCCAATTCGGATCGCATTTCAAAATCCAGTTTGATCCTAGCTAAAGACTA TCTGCATTCGGATATATCTCCTCATCAGACAGCAGGAGACCACCCTACTGTCTCTCCAAACTGCTTTGGC TCTCACCGGCAGTATTTTGACAAGCATGCTTACACATTAACTGGATATGCCCTGGAGCACTTATATGACA GCGAAACCATTAGAAACTATTCCTTGGGCTGTAATGGCTCACACTTTGATGTAACTTCCCATCTGAGGAT GCAACCAGACCCAGCACAAGGACACAAGGGAACATCTGTTATAATAACCAACGGAAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222805 representing NM_005068
Red=Cloning site Green=Tags(s) MKEKSKNAARTRREKENSEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRVVFPEGLGEAWGHSSRT SPLDNVGRELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVL TAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGL VAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCA HHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQISAS KPAFSYTSSSTPTMTDNRKGAKSRLSSSKSKSRTSPYPQYSGFHTERSESDHDSQWGGSPLTDTASPQLL DPADRPGSQHDASCAYRQFSDRSSLCYGFALDHSRLVEERHFHTQACEGGRCEAGRYFLGTPQAGREPWW GSRAALPLTKASPESREAYENSMPHIASVHRIHGRGHWDEDSVVSSPDPGSASESGDRYRTEQYQSSPHE PSKIETLIRATQQMIKEEENRLQLRKAPSDQLASINGAGKKHSLCFANYQQPPPTGEVCHGSALANTSPC DHIQQREGKMLSPHENDYDNSPTALSRISSPNSDRISKSSLILAKDYLHSDISPHQTAGDHPTVSPNCFG SHRQYFDKHAYTLTGYALEHLYDSETIRNYSLGCNGSHFDVTSHLRMQPDPAQGHKGTSVIITNGS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005068 |
| ORF Size | 2298 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005068.3 |
| RefSeq Size | 3995 bp |
| RefSeq ORF | 2301 bp |
| Locus ID | 6492 |
| UniProt ID | P81133 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 85.3 kDa |
| Gene Summary | SIM1 and SIM2 genes are Drosophila single-minded (sim) gene homologs. SIM1 transcript was detected only in fetal kidney out of various adult and fetal tissues tested. Since the sim gene plays an important role in Drosophila development and has peak levels of expression during the period of neurogenesis,it was proposed that the human SIM gene is a candidate for involvement in certain dysmorphic features (particularly the facial and skull characteristics), abnormalities of brain development, and/or cognitive disability of Down syndrome. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222805L3 | Lenti ORF clone of Human single-minded homolog 1 (Drosophila) (SIM1), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC222805L4 | Lenti ORF clone of Human single-minded homolog 1 (Drosophila) (SIM1), mGFP tagged |
CNY 7,700.00 |
|
| RG222805 | SIM1 (tGFP-tagged) - Human single-minded homolog 1 (Drosophila) (SIM1) |
CNY 6,370.00 |
|
| SC303573 | SIM1 (untagged)-Human single-minded homolog 1 (Drosophila) (SIM1) |
CNY 8,424.00 |
