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Ataxin 1 (ATXN1) (NM_000332) Human Tagged ORF Clone
CAT＃: RC222862
ATXN1 (Myc-DDK-tagged)-Human ataxin 1 (ATXN1), transcript variant 1
ORF Plasmid: tGFP
"NM_000332" in other vectors (6)
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CNY 5,968.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ATX1; D6S504E; SCA1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222862 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATCCAACCAAGAGCGGAGCAACGAATGCCTGCCTCCCAAGAAGCGCGAGATCCCCGCCACCAGCC GGTCCTCCGAGGAGAAGGCCCCTACCCTGCCCAGCGACAACCACCGGGTGGAGGGCACAGCATGGCTCCC GGGCAACCCTGGTGGCCGGGGCCACGGGGGCGGGAGGCATGGGCCGGCAGGGACCTCGGTGGAGCTTGGT TTACAACAGGGAATAGGTTTACACAAAGCATTGTCCACAGGGCTGGACTACTCCCCGCCCAGCGCTCCCA GGTCTGTCCCCGTGGCCACCACGCTGCCTGCCGCGTACGCCACCCCGCAGCCAGGGACCCCGGTGTCCCC CGTGCAGTACGCTCACCTGCCGCACACCTTCCAGTTCATTGGGTCCTCCCAATACAGTGGAACCTATGCC AGCTTCATCCCATCACAGCTGATCCCCCCAACCGCCAACCCCGTCACCAGTGCAGTGGCCTCGGCCGCAG GGGCCACCACTCCATCCCAGCGCTCCCAGCTGGAGGCCTATTCCACTCTGCTGGCCAACATGGGCAGTCT GAGCCAGACGCCGGGACACAAGGCTGAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCATCAG CATCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCACCTCAGCAGGGCTCCGGGGCTCA TCACCCCGGGGTCCCCCCCACCAGCCCAGCAGAACCAGTACGTCCACATTTCCAGTTCTCCGCAGAACAC CGGCCGCACCGCCTCTCCTCCGGCCATCCCCGTCCACCTCCACCCCCACCAGACGATGATCCCACACACG CTCACCCTGGGGCCCCCCTCCCAGGTCGTCATGCAATACGCCGACTCCGGCAGCCACTTTGTCCCTCGGG AGGCCACCAAGAAAGCTGAGAGCAGCCGGCTGCAGCAGGCCATCCAGGCCAAGGAGGTCCTGAACGGTGA GATGGAGAAGAGCCGGCGGTACGGGGCCCCGTCCTCAGCCGACCTGGGCCTGGGCAAGGCAGGCGGCAAG TCGGTTCCTCACCCGTACGAGTCCAGGCACGTGGTGGTCCACCCGAGCCCCTCAGACTACAGCAGTCGTG ATCCTTCGGGGGTCCGGGCCTCTGTGATGGTCCTGCCCAACAGCAACACGCCCGCAGCTGACCTGGAGGT GCAACAGGCCACTCATCGTGAAGCCTCCCCTTCTACCCTCAACGACAAAAGTGGCCTGCATTTAGGGAAG CCTGGCCACCGGTCCTACGCGCTCTCACCCCACACGGTCATTCAGACCACACACAGTGCTTCAGAGCCAC TCCCGGTGGGACTGCCAGCCACGGCCTTCTACGCAGGGACTCAACCCCCTGTCATCGGCTACCTGAGCGG CCAGCAGCAAGCAATCACCTACGCCGGCAGCCTGCCCCAGCACCTGGTGATCCCCGGCACACAGCCCCTG CTCATCCCGGTCGGCAGCACTGACATGGAAGCGTCGGGGGCAGCCCCGGCCATAGTCACGTCATCCCCCC AGTTTGCTGCAGTGCCTCACACGTTCGTCACCACCGCCCTTCCCAAGAGCGAGAACTTCAACCCTGAGGC CCTGGTCACCCAGGCCGCCTACCCAGCCATGGTGCAGGCCCAGATCCACCTGCCTGTGGTGCAGTCCGTG GCCTCCCCGGCGGCGGCTCCCCCTACGCTGCCTCCCTACTTCATGAAAGGCTCCATCATCCAGTTGGCCA ACGGGGAGCTAAAGAAGGTGGAAGACTTAAAAACAGAAGATTTCATCCAGAGTGCAGAGATAAGCAACGA CCTGAAGATCGACTCCAGCACCGTAGAGAGGATTGAAGACAGCCATAGCCCGGGCGTGGCCGTGATACAG TTCGCCGTCGGGGAGCACCGAGCCCAGGTCAGCGTTGAAGTTTTGGTAGAGTATCCTTTTTTTGTGTTTG GACAGGGCTGGTCATCCTGCTGTCCGGAGAGAACCAGCCAGCTCTTTGATTTGCCGTGTTCCAAACTCTC AGTTGGGGATGTCTGCATCTCGCTTACCCTCAAGAACCTGAAGAACGGCTCTGTTAAAAAGGGCCAGCCC GTGGATCCCGCCAGCGTCCTGCTGAAGCACTCAAAGGCCGACGGCCTGGCGGGCAGCAGACACAGGTATG CCGAGCAGGAAAACGGAATCAACCAGGGGAGTGCCCAGATGCTCTCTGAGAATGGCGAACTGAAGTTTCC AGAGAAAATGGGATTGCCTGCAGCGCCCTTCCTCACCAAAATAGAACCCAGCAAGCCCGCGGCAACGAGG AAGAGGAGGTGGTCGGCGCCAGAGAGCCGCAAACTGGAGAAGTCAGAAGACGAACCACCTTTGACTCTTC CTAAGCCTTCTCTAATTCCTCAGGAGGTTAAGATTTGCATTGAAGGCCGGTCTAATGTAGGCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222862 protein sequence
Red=Cloning site Green=Tags(s) MKSNQERSNECLPPKKREIPATSRSSEEKAPTLPSDNHRVEGTAWLPGNPGGRGHGGGRHGPAGTSVELG LQQGIGLHKALSTGLDYSPPSAPRSVPVATTLPAAYATPQPGTPVSPVQYAHLPHTFQFIGSSQYSGTYA SFIPSQLIPPTANPVTSAVASAAGATTPSQRSQLEAYSTLLANMGSLSQTPGHKAEQQQQQQQQQQQQHQ HQQQQQQQQQQQQQQHLSRAPGLITPGSPPPAQQNQYVHISSSPQNTGRTASPPAIPVHLHPHQTMIPHT LTLGPPSQVVMQYADSGSHFVPREATKKAESSRLQQAIQAKEVLNGEMEKSRRYGAPSSADLGLGKAGGK SVPHPYESRHVVVHPSPSDYSSRDPSGVRASVMVLPNSNTPAADLEVQQATHREASPSTLNDKSGLHLGK PGHRSYALSPHTVIQTTHSASEPLPVGLPATAFYAGTQPPVIGYLSGQQQAITYAGSLPQHLVIPGTQPL LIPVGSTDMEASGAAPAIVTSSPQFAAVPHTFVTTALPKSENFNPEALVTQAAYPAMVQAQIHLPVVQSV ASPAAAPPTLPPYFMKGSIIQLANGELKKVEDLKTEDFIQSAEISNDLKIDSSTVERIEDSHSPGVAVIQ FAVGEHRAQVSVEVLVEYPFFVFGQGWSSCCPERTSQLFDLPCSKLSVGDVCISLTLKNLKNGSVKKGQP VDPASVLLKHSKADGLAGSRHRYAEQENGINQGSAQMLSENGELKFPEKMGLPAAPFLTKIEPSKPAATR KRRWSAPESRKLEKSEDEPPLTLPKPSLIPQEVKICIEGRSNVGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000332 |
| ORF Size | 2445 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000332.3 |
| RefSeq Size | 10636 bp |
| RefSeq ORF | 2448 bp |
| Locus ID | 6310 |
| UniProt ID | P54253 |
| Domains | AXH |
| MW | 86.9 kDa |
| Gene Summary | The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the `pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. The function of the ataxins is not known. This locus has been mapped to chromosome 6, and it has been determined that the diseased allele contains 40-83 CAG repeats, compared to 6-39 in the normal allele, and is associated with spinocerebellar ataxia type 1 (SCA1). Alternative splicing results in multiple transcript variants, with one variant encoding multiple distinct proteins, ATXN1 and Alt-ATXN1, due to the use of overlapping alternate reading frames. [provided by RefSeq, Nov 2017] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Laminin signals initiate the reciprocal loop that informs breast-specific gene expression and homeostasis by activating NO, p53 and microRNAs
,Furuta, S;Ren, G;Mao, JH;Bissell, MJ;,
Elife
,PubMed ID 29560860
[Ataxin 1]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222862L1 | Lenti ORF clone of Human ataxin 1 (ATXN1), transcript variant 1, Myc-DDK-tagged |
CNY 8,368.00 |
|
| RC222862L2 | Lenti ORF clone of Human ataxin 1 (ATXN1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC222862L3 | Lenti ORF clone of Human ataxin 1 (ATXN1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222862L4 | Lenti ORF clone of Human ataxin 1 (ATXN1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG222862 | ATXN1 (tGFP-tagged) - Human ataxin 1 (ATXN1), transcript variant 1 |
CNY 4,370.00 |
|
| SC314762 | ATXN1 (untagged)-Human ataxin 1 (ATXN1), transcript variant 1 |
CNY 5,976.00 |
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