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DNASE1L2 (NM_001374) Human Tagged ORF Clone
CAT＃: RC222905
DNASE1L2 (Myc-DDK-tagged)-Human deoxyribonuclease I-like 2 (DNASE1L2)
ORF Plasmid: tGFP
"NM_001374" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DNAS1L2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222905 representing NM_001374
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGGGCCCCGGGCTCTGCTGGCCGCACTCTGGGCGCTGGAAGCCGCCGGGACCGCCGCGCTTCGCA TCGGAGCCTTCAACATTCAGAGCTTCGGTGACAGCAAAGTGTCGGACCCCGCTTGCGGCAGCATCATCGC GAAGATCCTGGCTGGCTATGACCTCGCGCTGGTGCAGGAGGTGCGAGACCCAGACCTCAGCGCCGTGTCC GCGCTCATGGAGCAGATCAACAGCGTGTCCGAGCACGAGTACAGCTTTGTGAGCAGCCAGCCCCTGGGCC GGGACCAGTACAAGGAGATGTACCTGTTCGTGTACAGGAAAGACGCGGTGTCGGTCGTGGACACCTACCT GTACCCAGACCCCGAGGACGTCTTCAGCCGCGAGCCCTTCGTGGTCAAGTTCTCGGCCCCCGGCACCGGT GAGCGGGCCCCGCCCCTCCCCTCCCGCCGAGCTCTGACGCCCCCACCCCTTCCCGCAGCAGCACAGAACC TGGTGCTGATCCCGCTGCACGCGGCGCCGCATCAAGCCGTGGCGGAGATCGACGCGCTCTACGACGTGTA CCTGGACGTGATCGACAAGTGGGGCACCGACGACATGCTGTTCCTGGGCGACTTCAACGCCGACTGCAGC TATGTGCGGGCGCAGGACTGGGCCGCCATCCGTCTGAGGAGCAGTGAGGTCTTCAAGTGGCTCATCCCTG ACAGCGCCGACACCACGGTGGGCAACTCAGACTGCGCCTACGACCGCATTGTGGCCTGTGGCGCCCGCCT GCGCCGGAGCCTGAAGCCCCAGTCGGCCACCGTGCACGACTTCCAGGAGGAATTCGGCCTGGACCAGACT CAGGCTCTTGCCATCAGCGACCACTTTCCAGTGGAGGTGACCCTCAAGTTCCACCGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222905 representing NM_001374
Red=Cloning site Green=Tags(s) MGGPRALLAALWALEAAGTAALRIGAFNIQSFGDSKVSDPACGSIIAKILAGYDLALVQEVRDPDLSAVS ALMEQINSVSEHEYSFVSSQPLGRDQYKEMYLFVYRKDAVSVVDTYLYPDPEDVFSREPFVVKFSAPGTG ERAPPLPSRRALTPPPLPAAAQNLVLIPLHAAPHQAVAEIDALYDVYLDVIDKWGTDDMLFLGDFNADCS YVRAQDWAAIRLRSSEVFKWLIPDSADTTVGNSDCAYDRIVACGARLRRSLKPQSATVHDFQEEFGLDQT QALAISDHFPVEVTLKFHR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001374 |
| ORF Size | 897 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001374.3 |
| RefSeq Size | 1365 bp |
| RefSeq ORF | 900 bp |
| Locus ID | 1775 |
| UniProt ID | Q92874 |
| Protein Families | Secreted Protein |
| MW | 32.7 kDa |
| Gene Summary | Divalent cation-dependent acid DNA endonuclease involved in the breakdown of the nucleus during corneocyte formation of epidermal keratinocytes. May play an immune role by eliminating harmful DNA released into the extracellular environment by damaged epidermal cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222905L3 | Lenti ORF clone of Human deoxyribonuclease I-like 2 (DNASE1L2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222905L4 | Lenti ORF clone of Human deoxyribonuclease I-like 2 (DNASE1L2), mGFP tagged |
CNY 5,890.00 |
|
| RG222905 | DNASE1L2 (tGFP-tagged) - Human deoxyribonuclease I-like 2 (DNASE1L2) |
CNY 4,370.00 |
|
| SC122592 | DNASE1L2 (untagged)-Human deoxyribonuclease I-like 2 (DNASE1L2) |
CNY 6,270.00 |
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