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BRD8 (NM_139199) Human Tagged ORF Clone
CAT＃: RC223002
BRD8 (Myc-DDK-tagged)-Human bromodomain containing 8 (BRD8), transcript variant 2
ORF Plasmid: tGFP
"NM_139199" in other vectors (6)
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CNY 8,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | p120; SMAP; SMAP2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223002 representing NM_139199
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACGGGAACGGGCAAACACAAGCTGCTAAGCACTGGCCCCACAGAGCCATGGTCCATCCGAGAGA AGCTATGTTTAGCATCTTCTGTCATGAGAAGTGGCGATCAAAATTGGGTATCAGTTAGCAGAGCAATCAA GCCCTTTGCAGAACCTGGCCGCCCTCCAGACTGGTTCTCTCAAAAACATTGTGCTTCCCAGTACTCGGAG CTTTTAGAGACCACTGAGACACCAAAACGGAAACGAGGTGAAAAGGGAGAAGTGGTGGAAACTGTTGAAG ATGTTATTGTTCGGAAATTGACTGCTGAGCGAGTTGAAGAACTAAAGAAAGTGATAAAGGAAACCCAGGA GAGATATAGACGGCTAAAGAGAGATGCAGAACTAATTCAAGCTGGACACATGGACAGCAGACTGGATGAG CTTTGCAATGACATTGCAACGAAAAAGAAATTGGAAGAAGAGGAGGCTGAAGTAAAGAGGAAGGCTACAG ATGCTGCATACCAGGCTCGTCAAGCAGTAAAAACACCCCCCCGGAGGTTACCCACTGTGATGGTTCGCTC TCCTATAGATTCTGCCTCCCCAGGAGGTGATTATCCACTTGGGGACTTGACTCCAACCACTATGGAAGAG GCTACCTCTGGGGTCAATGAGAGTGAAATGGCTGTGGCTTCTGGCCACCTGAACAGTACAGGTGTCCTCC TGGAGGTAGGCGGGGTCCTTCCCATGATACATGGTGGGGAGATACAGCAAACACCCAATACTGTTGCAGC CTCCCCTGCTGCATCAGGTGCTCCCACTCTTTCCCGGCTTTTAGAAGCTGGTCCTACACAGTTCACCACA CCTCTTGCTTCCTTCACTACTGTTGCCAGTGAGCCTCCAGTTAAACTTGTGCCACCCCCTGTAGAGTCTG TGTCCCAAGCTACCATTGTCATGATGCCTGCGCTGCCAGCACCATCCTCTGCTCCGGCTGTCTCCACTAC TGAAAGTGTAGCTCCAGTGAGTCAACCCGACAACTGTGTTCCCATGGAGGCTGTGGGGGATCCACATACT GTGACTGTTTCCATGGACAGCAGTGAAATATCCATGATCATCAATTCTATCAAAGAAGAGTGTTTTCGAT CAGGGGTAGCAGAGGCTCCTGTTGGATCAAAGGCTCCCAGCATAGATGGGAAGGAAGAATTAGATCTGGC TGAGAAGATGGATATTGCTGTGTCTTACACAGGTGAAGAGCTGGATTTTGAGACTGTTGGAGACATCATT GCCATCATTGAGGACAAGGTAGATGATCATCCTGAAGTGCTGGATGTGGCAGCAGTGGAAGCAGCACTGT CATTTTGTGAAGAAAATGATGATCCTCAGTCCCTGCCTGGCCCCTGGGAGCATCCTATCCAGCAGGAGCG GGACAAGCCAGTACCTCTCCCTGCACCAGAAATGACGGTCAAGCAAGAGAGACTGGACTTTGAGGAAACG GAAAACAAGGGAATACATGAACTGGTGGACATCAGGGAGCCCAGTGCAGAGATCAAGGTGGAACCTGCAG AACCAGAGCCAGTCATTTCAGGAGCCGAAATAGTAGCTGGAGTTGTTCCAGCCACAAGTATGGAGCCACC AGAACTCAGGAGTCAGGACTTAGATGAGGAACTGGGAAGTACTGCAGCTGGAGAGATTGTTGAAGCAGAT GTTGCCATTGGGAAAGGCGATGAGACTCCACTTACAAATGTGAAGACAGAGGCATCCCCTGAAAGCATGT TGTCTCCATCACATGGCTCAAATCCCATTGAAGATCCTTTAGAGGCAGAGACTCAGCACAAGTTTGAAAT GTCAGACTCATTGAAAGAAGAATCAGGGACTATTTTTGGAAGCCAGATAAAGGATGCCCCAGGTGAGGAT GAGGAGGAAGATGGTGTCAGTGAAGCGGCCAGCCTAGAGGAGCCTAAGGAAGAGGATCAAGGAGAAGGCT ACTTGTCAGAAATGGATAATGAACCTCCTGTGAGCGAGAGTGATGATGGCTTCAGCATACACAATGCTAC ACTGCAGTCACACACACTGGCAGACTCCATCCCCAGCAGCCCTGCTTCTTCACAGTTCTCTGTCTGTAGT GAGGATCAGGAAGCTATTCAGGCACAGAAAATTTGGAAGAAAGCCATCATGCTTGTATGGAGAGCTGCAG CTAATCATAGGTATGCCAATGTCTTCCTGCAGCCTGTTACAGATGACATAGCACCTGGCTACCACAGCAT TGTGCAGAGGCCTATGGATTTGTCAACTATTAAGAAAAACATAGAAAATGGACTGATCCGAAGCACAGCT GAATTTCAGCGTGACATTATGCTGATGTTTCAGAATGCTGTAATGTACAATAGCTCAGACCATGATGTCT ATCACATGGCAGTGGAGATGCAGCGAGATGTCTTGGAACAGATCCAGCAATTCTTGGCCACGCAGTTGAT TATGCAAACATCCGAGTCTGGGATCAGTGCTAAAAGTCTTCGAGGGAGAGATTCTACCCGCAAACAGGAT GCTTCAGAGAAGGACAGTGTCCCAATGGGCTCTCCTGCCTTCCTTCTCTCTCTCTTTATGGGACACGAGT GGGTTTGGCTGGATTCTGAACAAGATCATCCCAATGACTCTGAGTTGAGCAATGACTGCAGGTCCCTCTT CAGCTCATGGGACTCCAGTCTGGATCTTGATGTGGGCAACTGGAGGGAAACTGAGGATCCAGAGGCTGAG GAACTAGAGGAAAGCAGCCCGGAGAGAGAACCTAGTGAACTGCTTGTTGGGGATGGAGGCAGTGAGGAAT CTCAGGAAGCGGCAAGGAAAGCCAGCCACCAGAACCTCCTCCACTTTCTCTCTGAGGTAGCTTATTTAAT GGAGCCCTTGTGCATCAGCAGCAACGAATCAAGTGAAGGCTGCTGCCCTCCATCTGGTACCAGACAAGAA GGAAGGGAAATTAAAGCTAGCGAAGGAGAAAGGGAGCTCTGCAGAGAGACTGAAGAGCTTTCAGCTAAAG GAGACCCCTTAGTAGCTGAAAAGCCACTGGGAGAAAATGGAAAGCCAGAGGTGGCTTCAGCTCCCTCAGT TATTTGTACAGTTCAGGGACTACTCACAGAGAGTGAAGAGGGGGAGGCTCAGCAAGAATCCAAAGGGGAG GACCAGGGTGAAGTATATGTGTCAGAGATGGAAGACCAGCCCCCTTCAGGCGAGTGTGATGATGCCTTTA ACATTAAGGAGACTCCCTTGGTGGATACACTTTTCAGCCATGCTACCTCCTCAAAGCTGACTGATCTAAG CCAGGATGACCCTGTTCAGGATCATTTGCTATTTAAGAAGACTCTCCTGCCAGTCTGGAAGATGATTGCC AGTCACAGGTTCAGCAGTCCATTTCTGAAGCCTGTGTCAGAAAGGCAGGCCCCAGGGTACAAGGATGTGG TGAAAAGACCCATGGACTTAACTAGCCTGAAGAGAAATCTCTCTAAGGGTCGGATTCGCACCATGGCCCA ATTCCTGCGAGACCTGATGCTGATGTTCCAAAATGCTGTAATGTACAATGACTCTGATCATCATGTATAC CATATGGCTGTGGAGATGCGGCGAGAAGTCCTGGAGCAGATTCAGGTACTGAATATCTGGTTAGACAAAA GAAAAGGCTCAAGTAGTCTGGAAGGAGAACCAGCTAACCCAGTGGATGATGGAAAACCTGTTTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223002 representing NM_139199
Red=Cloning site Green=Tags(s) MATGTGKHKLLSTGPTEPWSIREKLCLASSVMRSGDQNWVSVSRAIKPFAEPGRPPDWFSQKHCASQYSE LLETTETPKRKRGEKGEVVETVEDVIVRKLTAERVEELKKVIKETQERYRRLKRDAELIQAGHMDSRLDE LCNDIATKKKLEEEEAEVKRKATDAAYQARQAVKTPPRRLPTVMVRSPIDSASPGGDYPLGDLTPTTMEE ATSGVNESEMAVASGHLNSTGVLLEVGGVLPMIHGGEIQQTPNTVAASPAASGAPTLSRLLEAGPTQFTT PLASFTTVASEPPVKLVPPPVESVSQATIVMMPALPAPSSAPAVSTTESVAPVSQPDNCVPMEAVGDPHT VTVSMDSSEISMIINSIKEECFRSGVAEAPVGSKAPSIDGKEELDLAEKMDIAVSYTGEELDFETVGDII AIIEDKVDDHPEVLDVAAVEAALSFCEENDDPQSLPGPWEHPIQQERDKPVPLPAPEMTVKQERLDFEET ENKGIHELVDIREPSAEIKVEPAEPEPVISGAEIVAGVVPATSMEPPELRSQDLDEELGSTAAGEIVEAD VAIGKGDETPLTNVKTEASPESMLSPSHGSNPIEDPLEAETQHKFEMSDSLKEESGTIFGSQIKDAPGED EEEDGVSEAASLEEPKEEDQGEGYLSEMDNEPPVSESDDGFSIHNATLQSHTLADSIPSSPASSQFSVCS EDQEAIQAQKIWKKAIMLVWRAAANHRYANVFLQPVTDDIAPGYHSIVQRPMDLSTIKKNIENGLIRSTA EFQRDIMLMFQNAVMYNSSDHDVYHMAVEMQRDVLEQIQQFLATQLIMQTSESGISAKSLRGRDSTRKQD ASEKDSVPMGSPAFLLSLFMGHEWVWLDSEQDHPNDSELSNDCRSLFSSWDSSLDLDVGNWRETEDPEAE ELEESSPEREPSELLVGDGGSEESQEAARKASHQNLLHFLSEVAYLMEPLCISSNESSEGCCPPSGTRQE GREIKASEGERELCRETEELSAKGDPLVAEKPLGENGKPEVASAPSVICTVQGLLTESEEGEAQQESKGE DQGEVYVSEMEDQPPSGECDDAFNIKETPLVDTLFSHATSSKLTDLSQDDPVQDHLLFKKTLLPVWKMIA SHRFSSPFLKPVSERQAPGYKDVVKRPMDLTSLKRNLSKGRIRTMAQFLRDLMLMFQNAVMYNDSDHHVY HMAVEMRREVLEQIQVLNIWLDKRKGSSSLEGEPANPVDDGKPVF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_139199 |
| ORF Size | 3705 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_139199.1, NP_631938.1 |
| RefSeq Size | 4074 bp |
| RefSeq ORF | 3708 bp |
| Locus ID | 10902 |
| UniProt ID | Q9H0E9 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 135.2 kDa |
| Gene Summary | The protein encoded by this gene interacts with thyroid hormone receptor in a ligand-dependent manner and enhances thyroid hormone-dependent activation from thyroid response elements. This protein contains a bromodomain and is thought to be a nuclear receptor coactivator. Multiple alternatively spliced transcript variants that encode distinct isoforms have been identified. [provided by RefSeq, Jul 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223002L1 | Lenti ORF clone of Human bromodomain containing 8 (BRD8), transcript variant 2, Myc-DDK-tagged |
CNY 11,000.00 |
|
| RC223002L2 | Lenti ORF clone of Human bromodomain containing 8 (BRD8), transcript variant 2, mGFP tagged |
CNY 14,250.00 |
|
| RC223002L3 | Lenti ORF clone of Human bromodomain containing 8 (BRD8), transcript variant 2, Myc-DDK-tagged |
CNY 14,250.00 |
|
| RC223002L4 | Lenti ORF clone of Human bromodomain containing 8 (BRD8), transcript variant 2, mGFP tagged |
CNY 14,250.00 |
|
| RG223002 | BRD8 (tGFP-tagged) - Human bromodomain containing 8 (BRD8), transcript variant 2 |
CNY 13,590.00 |
|
| SC125536 | BRD8 (untagged)-Human bromodomain containing 8 (BRD8), transcript variant 2 |
CNY 8,608.00 |
