GPR 150 (GPR150) (NM_199243) Human Tagged ORF Clone
CAT#: RC223227
GPR150 (Myc-DDK-tagged)-Human G protein-coupled receptor 150 (GPR150)
ORF Plasmid: tGFP
"NM_199243" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | PGR11 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC223227 representing NM_199243
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGATCTCTTTAGCCCCTCAATTCTGCCGCCGGCGCCCAACATTTCCGTGCCCATCTTGCTGGGCT GGGGTCTCAACCTGACCTTGGGGCAAGGAGCCCCTGCCTCTGGGCCGCCCAGCCGCCGCGTCCGCCTGGT GTTCCTGGGGGTCATCCTGGTGGTGGCGGTGGCAGGCAACACCACAGTGCTGTGCCGCCTGTGCGGCGGC GGCGGGCCCTGGGCGGGCCCCAAGCGTCGCAAGATGGACTTCCTGCTGGTGCAGCTGGCCCTGGCGGACC TGTACGCGTGCGGGGGCACGGCGCTGTCACAGCTGGCCTGGGAACTGCTGGGCGAGCCCCGCGCGGCCAC GGGGGACCTGGCGTGCCGCTTCCTGCAGCTGCTGCAGGCATCCGGGCGGGGCGCCTCGGCCCACCTCGTG GTGCTCATCGCCCTCGAGCGCCGGCGCGCGGTGCGTCTTCCGCACGGCCGGCCGCTGCCCGCGCGTGCCC TCGCCGCCCTGGGCTGGCTGCTGGCACTGCTGCTGGCGCTGCCCCCGGCCTTCGTGGTGCGCGGGGACTC CCCCTCGCCGCTGCCGCCGCCGCCGCCGCCAACGTCCCTGCAGCCAGGCGCGCCCCCGGCCGCCCGCGCC TGGCCGGGGGAGCGTCGCTGCCACGGGATCTTCGCGCCCCTGCCGCGCTGGCACCTGCAGGTCTACGCGT TCTACGAGGCCGTCGCGGGCTTCGTCGCGCCTGTTACGGTCCTGGGCGTCGCTTGCGGCCACCTACTCTC CGTCTGGTGGCGGCACCGGCCGCAGGCCCCCGCGGCTGCAGCGCCCTGGTCGGCGAGCCCAGGTCGAGCC CCTGCGCCCAGCGCGCTGCCCCGCGCCAAGGTGCAGAGCCTGAAGATGAGCCTGCTGCTGGCGCTGCTGT TCGTGGGCTGCGAGCTGCCCTACTTTGCCGCCCGGCTGGCGGCCGCGTGGTCGTCCGGGCCCGCGGGAGA CTGGGAGGGAGAGGGCCTGTCGGCGGCGCTGCGCGTGGTGGCGATGGCCAACAGCGCTCTCAATCCCTTC GTCTACCTCTTCTTCCAGGCGGGCGACTGCCGGCTCCGGCGACAGCTGCGGAAGCGGCTGGGCTCTCTGT GCTGCGCGCCGCAGGGAGGCGCGGAGGACGAGGAGGGGCCCCGGGGCCACCAGGCGCTCTACCGCCAACG CTGGCCCCACCCTCATTATCACCATGCTCGGCGGGAACCGCTGGACGAGGGCGGCTTGCGCCCACCCCCT CCGCGCCCCAGACCCCTGCCTTGCTCCTGCGAAAGTGCCTTC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC223227 representing NM_199243
Red=Cloning site Green=Tags(s) MEDLFSPSILPPAPNISVPILLGWGLNLTLGQGAPASGPPSRRVRLVFLGVILVVAVAGNTTVLCRLCGG GGPWAGPKRRKMDFLLVQLALADLYACGGTALSQLAWELLGEPRAATGDLACRFLQLLQASGRGASAHLV VLIALERRRAVRLPHGRPLPARALAALGWLLALLLALPPAFVVRGDSPSPLPPPPPPTSLQPGAPPAARA WPGERRCHGIFAPLPRWHLQVYAFYEAVAGFVAPVTVLGVACGHLLSVWWRHRPQAPAAAAPWSASPGRA PAPSALPRAKVQSLKMSLLLALLFVGCELPYFAARLAAAWSSGPAGDWEGEGLSAALRVVAMANSALNPF VYLFFQAGDCRLRRQLRKRLGSLCCAPQGGAEDEEGPRGHQALYRQRWPHPHYHHARREPLDEGGLRPPP PRPRPLPCSCESAF SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-RsrII Cloning Scheme for this gene Plasmid Map |
ACCN | NM_199243 |
ORF Size | 1302 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_199243.3 |
RefSeq Size | 1305 bp |
RefSeq ORF | 1305 bp |
Locus ID | 285601 |
UniProt ID | Q8NGU9 |
Protein Families | Druggable Genome, Transmembrane |
MW | 46.2 kDa |
Gene Summary | This gene encodes an orphan member of the class A rhodopsin-like family of G-protein-coupled receptors (GPCRs). Within the rhodopsin-like family, this gene is a member of the vasopressin-like subfamily that also includes vasopressin and oxytocin receptors. The silencing of this gene, due to promoter methylation, is associated with ovarian cancer progression. All GPCRs have a transmembrane domain that includes seven transmembrane alpha-helices. A general feature of GPCR signaling is the agonist-induced conformational change in the receptor, leading to activation of the heterotrimeric G protein. The activated G protein then binds to and activates numerous downstream effector proteins, which generate second messengers that mediate a broad range of cellular and physiological processes. [provided by RefSeq, Jul 2017] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC223227L3 | Lenti ORF clone of Human G protein-coupled receptor 150 (GPR150), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC223227L4 | Lenti ORF clone of Human G protein-coupled receptor 150 (GPR150), mGFP tagged |
CNY 5,890.00 |
|
RG223227 | GPR150 (tGFP-tagged) - Human G protein-coupled receptor 150 (GPR150) |
CNY 5,256.00 |
|
SC307908 | GPR150 (untagged)-Human G protein-coupled receptor 150 (GPR150) |
CNY 3,656.00 |
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