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Doppel (PRND) (NM_012409) Human Tagged ORF Clone
CAT＃: RC223341
- TrueORF®
PRND (Myc-DDK-tagged)-Human prion protein 2 (dublet) (PRND)
ORF Plasmid: tGFP
"NM_012409" in other vectors (4)
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CNY 3,600.00
CNY 4,180.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | dJ1068H6.4; DOPPEL; DPL; PrPLP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223341 representing NM_012409
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAAGCACCTGAGCTGGTGGTGGCTGGCCACTGTCTGCATGCTGCTCTTCAGCCACCTCTCTGCGG TCCAGACGAGGGGCATCAAGCACAGAATCAAGTGGAACCGGAAGGCCCTGCCCAGCACTGCCCAGATCAC TGAGGCCCAGGTGGCTGAGAACCGCCCGGGAGCCTTCATCAAGCAAGGCCGCAAGCTCGACATTGACTTC GGAGCCGAGGGCAACAGGTACTACGAGGCCAACTACTGGCAGTTCCCCGATGGCATCCACTACAACGGCT GCTCTGAGGCTAATGTGACCAAGGAGGCATTTGTCACCGGCTGCATCAATGCCACCCAGGCGGCGAACCA GGGGGAGTTCCAGAAGCCAGACAACAAGCTCCACCAGCAGGTGCTCTGGCGGCTGGTCCAGGAGCTCTGC TCCCTCAAGCATTGCGAGTTTTGGTTGGAGAGGGGCGCAGGACTTCGGGTCACCATGCACCAGCCAGTGC TCCTCTGCCTTCTGGCTTTGATCTGGCTCACGGTGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223341 representing NM_012409
Red=Cloning site Green=Tags(s) MRKHLSWWWLATVCMLLFSHLSAVQTRGIKHRIKWNRKALPSTAQITEAQVAENRPGAFIKQGRKLDIDF GAEGNRYYEANYWQFPDGIHYNGCSEANVTKEAFVTGCINATQAANQGEFQKPDNKLHQQVLWRLVQELC SLKHCEFWLERGAGLRVTMHQPVLLCLLALIWLTVK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012409 |
| ORF Size | 528 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012409.4 |
| RefSeq Size | 4047 bp |
| RefSeq ORF | 531 bp |
| Locus ID | 23627 |
| UniProt ID | Q9UKY0 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 20.29 kDa |
| Gene Summary | This gene is found on chromosome 20, approximately 20 kbp downstream of the gene encoding cellular prion protein, to which it is biochemically and structurally similar. The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that is found predominantly in testis. Mutations in this gene may lead to neurological disorders. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223341L3 | Lenti ORF clone of Human prion protein 2 (dublet) (PRND), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC223341L4 | Lenti ORF clone of Human prion protein 2 (dublet) (PRND), mGFP tagged |
CNY 6,000.00 |
|
| RG223341 | PRND (tGFP-tagged) - Human prion protein 2 (dublet) (PRND) |
CNY 5,200.00 |
|
| SC303977 | PRND (untagged)-Human prion protein 2 (dublet) (PRND) |
CNY 3,600.00 |
