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FANCD2 (NM_033084) Human Tagged ORF Clone
CAT＃: RC223476
- TrueORF®
FANCD2 (Myc-DDK-tagged)-Human Fanconi anemia, complementation group D2 (FANCD2), transcript variant 1
ORF Plasmid: tGFP
"NM_033084" in other vectors (4)
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CNY 15,096.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FA-D2; FA4; FACD; FAD; FAD2; FANCD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223476 representing NM_033084
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTTCCAAAAGAAGACTGTCAAAATCTGAGGATAAAGAGAGCCTGACAGAAGATGCCTCCAAAACCA GGAAGCAACCACTTTCCAAAAAGACAAAGAAATCTCATATTGCTAATGAAGTTGAAGAAAATGACAGCAT CTTTGTAAAGCTTCTTAAGATATCAGGAATTATTCTTAAAACGGGAGAGAGTCAGAATCAACTAGCTGTG GATCAAATAGCTTTCCAAAAGAAGCTCTTTCAGACCCTGAGGAGACACCCTTCCTATCCCAAAATAATAG AAGAATTTGTTAGTGGCCTGGAGTCTTACATTGAGGATGAAGACAGTTTCAGGAACTGCCTTTTGTCTTG TGAGCGTCTGCAGGATGAGGAAGCCAGTATGGGTGCATCTTATTCTAAGAGTCTCATCAAACTGCTTCTG GGGATTGACATACTGCAGCCTGCCATTATCAAAACCTTATTTGAGAAGTTGCCAGAATATTTTTTTGAAA ACAAGAACAGTGATGAAATCAACATACCTCGACTCATTGTCAGTCAACTAAAATGGCTTGACAGAGTTGT GGATGGCAAGGACCTCACCACCAAGATCATGCAGCTGATCAGTATTGCTCCAGAGAACCTGCAGCATGAC ATCATCACCAGCCTACCTGAGATCCTAGGGGATTCCCAGCACGCTGATGTGGGGAAAGAACTCAGTGACC TACTGATAGAGAATACTTCACTCACTGTCCCAATCCTGGATGTCCTTTCAAGCCTCCGACTTGACCCAAA CTTCCTATTGAAGGTTCGCCAGTTGGTGATGGATAAGTTGTCGTCTATTAGATTGGAGGATTTACCTGTG ATAATAAAGTTCATTCTTCATTCCGTAACAGCCATGGATACACTTGAGGTAATTTCTGAGCTTCGGGAGA AGTTGGATCTGCAGCATTGTGTTTTGCCATCACGGTTACAGGCTTCCCAAGTAAAGTTGAAAAGTAAAGG ACGAGCAAGTTCCTCAGGAAATCAAGAAAGCAGCGGTCAGAGCTGTATTATTCTCCTCTTTGATGTAATA AAGTCAGCTATTAGATATGAGAAAACCATTTCAGAAGCCTGGATTAAGGCAATTGAAAACACTGCCTCAG TATCTGAACACAAGGTGTTTGACCTGGTGATGCTTTTCATCATCTATAGCACCAATACTCAGACAAAGAA GTACATTGACAGGGTGCTAAGAAATAAGATTCGATCAGGCTGCATTCAAGAACAGCTGCTCCAGAGTACA TTCTCTGTTCATTACTTAGTTCTTAAGGATATGTGTTCATCCATTCTGTCGCTGGCTCAGAGTTTGCTTC ACTCTCTAGACCAGAGTATAATTTCATTTGGCAGTCTCCTATACAAATATGCATTTAAGTTTTTTGACAC GTACTGCCAGCAGGAAGTGGTTGGTGCCTTAGTGACCCATATCTGCAGTGGGAATGAAGCTGAAGTTGAT ACTGCCTTAGATGTCCTTCTAGAGTTGGTAGTGTTAAACCCATCTGCTATGATGATGAATGCTGTCTTTG TAAAGGGCATTTTAGATTATCTGGATAACATATCCCCTCAGCAAATACGAAAACTCTTCTATGTTCTCAG CACACTGGCATTTAGCAAACAGAATGAAGCCAGCAGCCACATCCAGGATGACATGCACTTGGTGATAAGA AAGCAGCTCTCTAGCACCGTATTCAAGTACAAGCTCATTGGGATTATTGGTGCTGTGACCATGGCTGGCA TCATGGCGGCAGACAGAAGTGAATCACCTAGTTTGACCCAAGAGAGAGCCAACCTGAGCGATGAGCAGTG CACACAGGTGACCTCCTTGTTGCAGTTGGTTCATTCCTGCAGTGAGCAGTCTCCTCAGGCCTCTGCACTT TACTATGATGAATTTGCCAACCTGATCCAACATGAAAAGCTGGATCCAAAAGCCCTGGAATGGGTTGGGC ATACCATCTGTAATGATTTCCAGGATGCCTTCGTAGTGGACTCCTGTGTTGTTCCGGAAGGTGACTTTCC ATTTCCTGTGAAAGCACTGTACGGACTGGAAGAATACGACACTCAGGATGGGATTGCCATAAACCTCCTG CCGCTGCTGTTTTCTCAGGACTTTGCAAAAGATGGGGGTCCGGTGACCTCACAGGAATCAGGCCAAAAAT TGGTGTCTCCGCTGTGCCTGGCTCCGTATTTCCGGTTACTGAGACTTTGTGTGGAGAGACAGCATAACGG AAACTTGGAGGAGATTGATGGTCTACTAGATTGTCCTATATTCCTAACTGACCTGGAGCCTGGAGAGAAG TTGGAGTCCATGTCTGCTAAAGAGCGTTCATTCATGTGTTCTCTCATATTTCTTACTCTCAACTGGTTCC GAGAGATTGTAAATGCCTTCTGCCAGGAAACATCACCTGAGATGAAGGGGAAGGTGCTCACTCGGTTAAA GCACATTGTAGAATTGCAAATAATCCTGGAAAAGTACTTGGCAGTCACCCCAGACTATGTCCCTCCTCTT GGAAACTTTGATGTGGAAACTTTAGATATAACACCTCATACTGTTACTGCTATTTCAGCAAAAATCAGAA AGAAAGGAAAAATAGAAAGGAAACAAAAAACAGATGGCAGCAAGACATCCTCCTCTGACACACTTTCAGA AGAGAAAAATTCAGAATGTGACCCTACGCCATCTCATAGAGGCCAGCTAAACAAGGAGTTCACAGGGAAG GAAGAAAAGACATCATTGTTACTACATAATTCCCATGCTTTTTTCCGAGAGCTGGACATTGAGGTCTTCT CTATTCTACATTGTGGACTTGTGACGAAGTTCATCTTAGATACTGAAATGCACACTGAAGCTACAGAAGT TGTGCAACTTGGGCCCCCTGAGCTGCTTTTCTTGCTGGAAGATCTCTCCCAGAAGCTGGAGAGTATGCTG ACACCTCCTATTGCCAGGAGAGTCCCCTTTCTCAAGAACAAAGGAAGCCGGAATATTGGATTCTCACATC TCCAACAGAGATCTGCCCAAGAAATTGTTCATTGTGTTTTTCAACTGCTGACCCCAATGTGTAACCACCT GGAGAACATTCACAACTATTTTCAGTGTTTAGCTGCTGAGAATCACGGTGTAGTTGATGGACCAGGAGTG AAAGTTCAGGAGTACCACATAATGTCTTCCTGCTATCAGAGGCTGCTGCAGATTTTTCATGGGCTTTTTG CTTGGAGTGGATTTTCTCAACCTGAAAATCAGAATTTACTGTATTCAGCCCTCCATGTCCTTAGTAGCCG ACTGAAACAGGGAGAACACAGCCAGCCTTTGGAGGAACTACTCAGCCAGAGCGTCCATTACTTGCAGAAT TTCCATCAAAGCATTCCCAGTTTCCAGTGTGCTCTTTATCTCATCAGACTTTTGATGGTTATTTTGGAGA AATCAACAGCTTCTGCTCAGAACAAAGAAAAAATTGCTTCCCTTGCCAGACAATTCCTCTGTCGGGTGTG GCCAAGTGGGGATAAAGAGAAGAGCAACATCTCTAATGACCAGCTCCATGCTCTGCTCTGTATCTACCTG GAGCACACAGAGAGCATTCTGAAGGCCATAGAGGAGATTGCTGGTGTTGGTGTCCCAGAACTGATCAACT CTCCTAAAGATGCATCTTCCTCCACATTCCCTACACTGACCAGGCATACTTTTGTTGTTTTCTTCCGTGT GATGATGGCTGAACTAGAGAAGACGGTGAAAAAAATTGAGCCTGGCACAGCAGCAGACTCGCAGCAGATT CATGAAGAGAAACTCCTCTACTGGAACATGGCTGTTCGAGACTTCAGTATCCTCATCAACTTGATAAAGG TATTTGATAGTCATCCTGTTCTGCATGTATGTTTGAAGTATGGGCGTCTCTTTGTGGAAGCATTTCTGAA GCAATGTATGCCGCTCCTAGACTTCAGTTTTAGAAAACACCGGGAAGATGTTCTGAGCTTACTGGAAACC TTCCAGTTGGACACAAGGCTGCTTCATCACCTGTGTGGGCATTCCAAGATTCACCAGGACACGAGACTCA CCCAACATGTGCCTCTGCTCAAAAAGACCCTGGAACTTTTAGTTTGCAGAGTCAAAGCTATGCTCACTCT CAACAATTGTAGAGAGGCTTTCTGGCTGGGCAATCTAAAAAACCGGGACTTGCAGGGTGAAGAGATTAAG TCCCAAAATTCCCAGGAGAGCACAGCAGATGAGAGTGAGGATGACATGTCATCCCAGGCCTCCAAGAGCA AAGCCACTGAGGTATCTCTACAAAACCCACCAGAGTCTGGCACTGATGGTTGCATTTTGTTAATTGTTCT AAGTTGGTGGAGCAGAACTTTGCCTACTTATGTTTATTGTCAAATGCTTCTATGCCCATTTCCATTCCCT CCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223476 representing NM_033084
Red=Cloning site Green=Tags(s) MVSKRRLSKSEDKESLTEDASKTRKQPLSKKTKKSHIANEVEENDSIFVKLLKISGIILKTGESQNQLAV DQIAFQKKLFQTLRRHPSYPKIIEEFVSGLESYIEDEDSFRNCLLSCERLQDEEASMGASYSKSLIKLLL GIDILQPAIIKTLFEKLPEYFFENKNSDEINIPRLIVSQLKWLDRVVDGKDLTTKIMQLISIAPENLQHD IITSLPEILGDSQHADVGKELSDLLIENTSLTVPILDVLSSLRLDPNFLLKVRQLVMDKLSSIRLEDLPV IIKFILHSVTAMDTLEVISELREKLDLQHCVLPSRLQASQVKLKSKGRASSSGNQESSGQSCIILLFDVI KSAIRYEKTISEAWIKAIENTASVSEHKVFDLVMLFIIYSTNTQTKKYIDRVLRNKIRSGCIQEQLLQST FSVHYLVLKDMCSSILSLAQSLLHSLDQSIISFGSLLYKYAFKFFDTYCQQEVVGALVTHICSGNEAEVD TALDVLLELVVLNPSAMMMNAVFVKGILDYLDNISPQQIRKLFYVLSTLAFSKQNEASSHIQDDMHLVIR KQLSSTVFKYKLIGIIGAVTMAGIMAADRSESPSLTQERANLSDEQCTQVTSLLQLVHSCSEQSPQASAL YYDEFANLIQHEKLDPKALEWVGHTICNDFQDAFVVDSCVVPEGDFPFPVKALYGLEEYDTQDGIAINLL PLLFSQDFAKDGGPVTSQESGQKLVSPLCLAPYFRLLRLCVERQHNGNLEEIDGLLDCPIFLTDLEPGEK LESMSAKERSFMCSLIFLTLNWFREIVNAFCQETSPEMKGKVLTRLKHIVELQIILEKYLAVTPDYVPPL GNFDVETLDITPHTVTAISAKIRKKGKIERKQKTDGSKTSSSDTLSEEKNSECDPTPSHRGQLNKEFTGK EEKTSLLLHNSHAFFRELDIEVFSILHCGLVTKFILDTEMHTEATEVVQLGPPELLFLLEDLSQKLESML TPPIARRVPFLKNKGSRNIGFSHLQQRSAQEIVHCVFQLLTPMCNHLENIHNYFQCLAAENHGVVDGPGV KVQEYHIMSSCYQRLLQIFHGLFAWSGFSQPENQNLLYSALHVLSSRLKQGEHSQPLEELLSQSVHYLQN FHQSIPSFQCALYLIRLLMVILEKSTASAQNKEKIASLARQFLCRVWPSGDKEKSNISNDQLHALLCIYL EHTESILKAIEEIAGVGVPELINSPKDASSSTFPTLTRHTFVVFFRVMMAELEKTVKKIEPGTAADSQQI HEEKLLYWNMAVRDFSILINLIKVFDSHPVLHVCLKYGRLFVEAFLKQCMPLLDFSFRKHREDVLSLLET FQLDTRLLHHLCGHSKIHQDTRLTQHVPLLKKTLELLVCRVKAMLTLNNCREAFWLGNLKNRDLQGEEIK SQNSQESTADESEDDMSSQASKSKATEVSLQNPPESGTDGCILLIVLSWWSRTLPTYVYCQMLLCPFPFP P TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033084 |
| ORF Size | 4413 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033084.6 |
| RefSeq Size | 5204 bp |
| RefSeq ORF | 4416 bp |
| Locus ID | 2177 |
| UniProt ID | Q9BXW9 |
| Protein Families | Druggable Genome |
| MW | 166.3 kDa |
| Gene Summary | The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group D2. This protein is monoubiquinated in response to DNA damage, resulting in its localization to nuclear foci with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repair. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Homocysteine aggravates DNA damage by impairing the FA/Brca1 Pathway in NE4C murine neural stem cells
,Yan, Y;Yin, Y;Feng, X;Chen, Y;Shi, J;Weng, H;Wang, D;,
Int. J. Med. Sci.
,PubMed ID 33029090
[FANCD2]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223476L3 | Lenti-ORF clone of FANCD2 (Myc-DDK-tagged)-Human Fanconi anemia, complementation group D2 (FANCD2), transcript variant 1 |
CNY 16,530.00 |
|
| RC223476L4 | Lenti-ORF clone of FANCD2 (mGFP-tagged)-Human Fanconi anemia, complementation group D2 (FANCD2), transcript variant 1 |
CNY 16,530.00 |
|
| RG223476 | FANCD2 (tGFP-tagged) - Human Fanconi anemia, complementation group D2 (FANCD2), transcript variant 1 |
CNY 16,696.00 |
|
| SC305556 | FANCD2 (untagged)-Human Fanconi anemia, complementation group D2 (FANCD2), transcript variant 1 |
CNY 28,410.00 |
