CERKL (NM_201548) Human Tagged ORF Clone
CAT#: RC223592
CERKL (Myc-DDK-tagged)-Human ceramide kinase-like (CERKL), transcript variant 1
ORF Plasmid: tGFP
"NM_201548" in other vectors (6)
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CNY 3,960.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | RP26 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC223592 representing NM_201548
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTGGAGGAGGCGCAGGAACCGGGTGAGTGCCCTGGAGGGCGGCCGGGAGGAAGAGGCGCCCCCGG AGGCTGCCGCTGTGCCTCCGGCGCTGTTAACGTCCCCGCAGCAGACGGAGGCGGCGGCCGAGCGGATTCT GCTCCGGGGCATCTTCGAGATCGGGAGGGACAGTTGTGACGTGGTGCTGAGCGAGCGAGCACTGCGGTGG CGGCCCATTCAGCCCGAGCGCCCGGCGGGTGATTCTAAGTATGACTTGCTATGTAAAGAAGAATTTATTG AACTCAAAGACATATTCTCTGTGAAACTGAAACGGCGTTGTTCTGTTAAACAGCAGAGAAGTGGTACTTT ATTAGGTATCACACTCTTCATCTGCTTGAAAAAGGAACAAAATAAACTAAAGAATTCTACACTTGATCTT ATTAATTTAAGTGAAGACCACTGTGACATATGGTTTAGACAGTTCAAGAAAATATTGGCAGGCTTTCCAA ACAGACCGAAGTCATTAAAAATACTCCTTAACCCCCAAAGTCACAAAAAAGAAGCTACCCAGGTTTATTA TGAGAAGGTTGAACCTCTGTTGAAGCTTGCAGGAATAAAAACTGATGTAACAATAATGGAATATGAAGGG CACGCTCTGTCACTGCTTAAGGAATGTGAACTCCAGGGATTTGATGGTGTTGTCTGTGTTGGTGGAGATG GATCTGCTAGCGAAGTAGCCCATGCTTTGCTTCTGAGAGCTCAGAAGAATGCTGGGATGGAAACAGACCG AATCCTGACTCCTGTCAGAGCACAGCTTCCACTTGGCTTAATACCAGCAGGATCTACCAATGTATTGGCA CATTCTCTTCATGGAGTTCCTCATGTGATAACTGCAACATTGCACATTATAATGGGGCATGTACAGCTGG TCGACGTCTGCACCTTCAGCACCGCTGGCAAGCTTCTTCGCTTTGGGTTCTCAGCCATGTTTGGCTTTGG TGGAAGAACTTTGGCTCTGGCAGAAAAATATCGATGGATGTCCCCTAACCAACGGAGAGATTTTGCTGTT GTTAAGGCACTGGCAAAACTTAAGGCAGAAGACTGTGAAATATCATTTTTACCATTTAACAGCTCTGATG ATGTGCAAGAAAGGAGGGCACAGGGATCTCCCAAATCTGACTGTAATGATCAATGGCAAATGATCCAGGG TCAGTTCTTGAATGTCAGCATTATGGCAATTCCTTGCCTGTGTTCAGTGGCACCTAGAGGCTTGGCACCT AATACCAGATTAAATAATGGAAGTATGGCTCTTATAATTGCCCGAAACACTTCTCGGCCAGAATTTATAA AACACCTGAAAAGATATGCCAGTGTAAAAAATCAGTTCAATTTTCCATTTGTTGAGACTTACACTGTTGA GGAAGTAAAAGTTCATCCAAGGAATAATACTGGTGGATATAATCCAGAGGAGGAGGAGGATGAAACTGCT TCAGAAAATTGTTTCCCTTGGAATGTAGATGGTGATTTAATGGAAGTTGCATCAGAGGTCCATATTAGAT TGCATCCAAGACTTATCAGTCTTTATGGAGGAAGCATGGAAGAAATGATTCCAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223592 representing NM_201548
Red=Cloning site Green=Tags(s) MPWRRRRNRVSALEGGREEEAPPEAAAVPPALLTSPQQTEAAAERILLRGIFEIGRDSCDVVLSERALRW RPIQPERPAGDSKYDLLCKEEFIELKDIFSVKLKRRCSVKQQRSGTLLGITLFICLKKEQNKLKNSTLDL INLSEDHCDIWFRQFKKILAGFPNRPKSLKILLNPQSHKKEATQVYYEKVEPLLKLAGIKTDVTIMEYEG HALSLLKECELQGFDGVVCVGGDGSASEVAHALLLRAQKNAGMETDRILTPVRAQLPLGLIPAGSTNVLA HSLHGVPHVITATLHIIMGHVQLVDVCTFSTAGKLLRFGFSAMFGFGGRTLALAEKYRWMSPNQRRDFAV VKALAKLKAEDCEISFLPFNSSDDVQERRAQGSPKSDCNDQWQMIQGQFLNVSIMAIPCLCSVAPRGLAP NTRLNNGSMALIIARNTSRPEFIKHLKRYASVKNQFNFPFVETYTVEEVKVHPRNNTGGYNPEEEEDETA SENCFPWNVDGDLMEVASEVHIRLHPRLISLYGGSMEEMIPK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_201548 |
ORF Size | 1596 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_201548.3, NP_963842.1 |
RefSeq Size | 3123 bp |
RefSeq ORF | 1599 bp |
Locus ID | 375298 |
UniProt ID | Q49MI3 |
Protein Families | Druggable Genome |
MW | 59.4 kDa |
Gene Summary | This gene was initially identified as a locus (RP26) associated with an autosomal recessive form of retinitis pigmentosa (arRP) disease. This gene encodes a protein with ceramide kinase-like domains, however, the protein does not phosphorylate ceramide and its target substrate is currently unknown. This protein may be a negative regulator of apoptosis in photoreceptor cells. Mutations in this gene cause a form of retinitis pigmentosa characterized by autosomal recessive cone and rod dystrophy (arCRD). Alternative splicing of this gene results in multiple transcript variants encoding different isoforms and non-coding transcripts.[provided by RefSeq, May 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC223592L1 | Lenti ORF clone of Human ceramide kinase-like (CERKL), transcript variant 1, Myc-DDK-tagged |
CNY 6,360.00 |
|
RC223592L2 | Lenti ORF clone of Human ceramide kinase-like (CERKL), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC223592L3 | Lenti ORF clone of Human ceramide kinase-like (CERKL), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC223592L4 | Lenti ORF clone of Human ceramide kinase-like (CERKL), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG223592 | CERKL (tGFP-tagged) - Human ceramide kinase-like (CERKL), transcript variant 1 |
CNY 4,370.00 |
|
SC308051 | CERKL (untagged)-Human ceramide kinase-like (CERKL), transcript variant 1 |
CNY 7,220.00 |