GCHFR (NM_005258) Human Tagged ORF Clone
CAT#: RC223674
GCHFR (Myc-DDK-tagged)-Human GTP cyclohydrolase I feedback regulator (GCHFR)
ORF Plasmid: tGFP
"NM_005258" in other vectors (4)
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CNY 1,200.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | GFRP; HsT16933; P35 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC223674 representing NM_005258
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTACCTGCTCATCAGCACCCAGATCCGCATGGAGGTGGGCCCCACTATGGTGGGCGATGAACAGT CGGATCCAGAGCTGATGCAGCATCTGGGGGCTTCAAAGAGAAGAGCCTTGGGAAACAACTTTTATGAATA CTACGTCGATGACCCTCCCCGCATAGTCCTGGACAAGCTGGAACGCAGGGGCTTCCGTGTGCTGAGCATG ACGGGGGTGGGCCAGACGCTGGTGTGGTGTCTGCACAAGGAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC223674 representing NM_005258
Red=Cloning site Green=Tags(s) MPYLLISTQIRMEVGPTMVGDEQSDPELMQHLGASKRRALGNNFYEYYVDDPPRIVLDKLERRGFRVLSM TGVGQTLVWCLHKE SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-RsrII Cloning Scheme for this gene Plasmid Map |
ACCN | NM_005258 |
ORF Size | 252 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005258.3 |
RefSeq Size | 713 bp |
RefSeq ORF | 255 bp |
Locus ID | 2644 |
UniProt ID | P30047 |
MW | 9.5 kDa |
Gene Summary | GTP cyclohydrolase I feedback regulatory protein binds to and mediates tetrahydrobiopterin inhibition of GTP cyclohydrolase I. The regulatory protein, GCHFR, consists of a homodimer. It is postulated that GCHFR may play a role in regulating phenylalanine metabolism in the liver and in the production of biogenic amine neurotransmitters and nitric oxide. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC223674L3 | Lenti-ORF clone of GCHFR (Myc-DDK-tagged)-Human GTP cyclohydrolase I feedback regulator (GCHFR) |
CNY 5,890.00 |
|
RC223674L4 | Lenti-ORF clone of GCHFR (mGFP-tagged)-Human GTP cyclohydrolase I feedback regulator (GCHFR) |
CNY 5,890.00 |
|
RG223674 | GCHFR (tGFP-tagged) - Human GTP cyclohydrolase I feedback regulator (GCHFR) |
CNY 4,370.00 |
|
SC116878 | GCHFR (untagged)-Human GTP cyclohydrolase I feedback regulator (GCHFR) |
CNY 1,200.00 |