FBXO25 (NM_183420) Human Tagged ORF Clone
CAT#: RC223986
FBXO25 (Myc-DDK-tagged)-Human F-box protein 25 (FBXO25), transcript variant 2
ORF Plasmid: tGFP
"NM_183420" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | FBX25 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC223986 representing NM_183420
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCATTTTTGGGTCAGGACTGGAGATCTCCTGGATGGAGTTGGATTAAGACAGAAGATGGCTGGAAGA GATGTGAATCTTGTAGTCAGAAACTTGAAAGAGAGAATAACCGTTGTAACATCAGTCACAGCATTATCTT AAATAGTGAAGATGGAGAAATATTCAATAATGAAGAGCATGAATATGCATCGAAAAAAAGGAAAAAGGAC CATTTTAGAAATGACACAAATACTCAAAGTTTTTATCGTGAAAAATGGATCTATGTCCATAAAGAAAGCA CAAAGGAAAGGCATGGCTATTGCACCTTGGGAGAAGCCTTTAATCGGTTAGACTTCTCAAGTGCAATTCA AGATATCCGAAGGTTCAATTATGTGGTCAAACTGTTGCAGCTAATTGCAAAATCCCAGTTAACTTCATTG AGTGGCGTGGCACAGAAGAATTACTTCAACATTTTGGATAAAATCGTTCAAAAGGTTCTTGATGACCACC ACAATCCTCGCTTAATCAAAGATCTTCTGCAAGACCTAAGCTCTACCCTCTGCATTCTTATTAGAGGAGT AGGGAAGTCTGTATTAGTGGGAAACATCAATATTTGGATTTGCCGATTAGAAACTATTCTCGCCTGGCAA CAACAGCTACAGGATCTTCAGATGACTAAGCAAGTGAACAATGGCCTCACCCTCAGTGACCTTCCTCTGC ACATGCTGAACAACATCCTATACCGGTTCTCAGACGGATGGGACATCATCACCTTAGGCCAGGTGACCCC CACGTTGTATATGCTTAGTGAAGACAGACAGCTGTGGAAGAAGCTTTGTCAGTACCATTTTGCTGAAAAG CAGTTTTGTAGACATTTGATCCTTTCAGAAAAAGGTCATATTGAATGGAAGTTGATGTACTTTGCACTTC AGAAACATTACCCAGCGAAGGAGCAGTACGGAGACACACTGCATTTCTGTCGGCACTGCAGCATTCTCTT TTGGAAGGACTCAGGACACCCCTGCACGGCGGCCGACCCTGACAGCTGCTTCACGCCTGTGTCTCCGCAG CACTTCATCGACCTCTTCAAGTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223986 representing NM_183420
Red=Cloning site Green=Tags(s) MPFLGQDWRSPGWSWIKTEDGWKRCESCSQKLERENNRCNISHSIILNSEDGEIFNNEEHEYASKKRKKD HFRNDTNTQSFYREKWIYVHKESTKERHGYCTLGEAFNRLDFSSAIQDIRRFNYVVKLLQLIAKSQLTSL SGVAQKNYFNILDKIVQKVLDDHHNPRLIKDLLQDLSSTLCILIRGVGKSVLVGNINIWICRLETILAWQ QQLQDLQMTKQVNNGLTLSDLPLHMLNNILYRFSDGWDIITLGQVTPTLYMLSEDRQLWKKLCQYHFAEK QFCRHLILSEKGHIEWKLMYFALQKHYPAKEQYGDTLHFCRHCSILFWKDSGHPCTAADPDSCFTPVSPQ HFIDLFKF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_183420 |
ORF Size | 1074 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_183420.2 |
RefSeq Size | 2502 bp |
RefSeq ORF | 1077 bp |
Locus ID | 26260 |
UniProt ID | Q8TCJ0 |
MW | 42 kDa |
Gene Summary | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC223986L3 | Lenti ORF clone of Human F-box protein 25 (FBXO25), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC223986L4 | Lenti ORF clone of Human F-box protein 25 (FBXO25), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG223986 | FBXO25 (tGFP-tagged) - Human F-box protein 25 (FBXO25), transcript variant 2 |
CNY 4,370.00 |
|
SC313116 | FBXO25 (untagged)-Human F-box protein 25 (FBXO25), transcript variant 2 |
CNY 5,800.00 |