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anoctamin 5 (ANO5) (NM_213599) Human Tagged ORF Clone
CAT＃: RC224062
- TrueORF®
ANO5 (Myc-DDK-tagged)-Human anoctamin 5 (ANO5), transcript variant 1
ORF Plasmid: tGFP
"NM_213599" in other vectors (4)
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CNY 10,032.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GDD1; LGMD2L; LGMDR12; TMEM16E |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224062 representing NM_213599
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGACCCGGATCTCCTGGAAGTGTTGGCGGAGGAAGGGGAAAAAGTCAATAAGCATATAGACTACT CTTTCCAAATGAGTGAGCAGAGCCTGAGCAGCAGAGAGACCAGCTTTCTCATCAATGAAGAAACAATGCC TGCAAAGCGATTCAATTTGTTCCTGAGGCGGCGGCTTATGTTTCAAAAAAATCAGCAAAGCAAAGATTCT ATCTTCTTCCGAGATGGGATTAGGCAAATTGATTTTGTGCTTTCCTACGTTGATGATGTAAAGAAAGACG CAGAGTTAAAGGCGGAAAGAAGAAAAGAGTTTGAAACTAATCTCAGAAAAACAGGTCTTGAGTTGGAAAT AGAAGACAAAAGGGACTCGGAAGATGGAAGAACTTATTTTGTCAAGATCCATGCCCCTTGGGAGGTATTA GTTACCTATGCTGAAGTCTTGGGAATCAAAATGCCTATTAAGGAGAGTGATATTCCCCGCCCTAAGCACA CTCCTATAAGCTATGTGCTTGGACCTGTAAGACTCCCACTGAGTGTGAAGTATCCCCATCCTGAATATTT TACTGCACAATTCAGCAGACATCGGCAGGAGCTCTTCCTCATCGAAGATCAGGCAACCTTCTTTCCATCC TCATCAAGAAACAGAATTGTGTACTATATTCTCTCAAGATGTCCTTTTGGCATAGAAGATGGGAAGAAAA GGTTTGGGATTGAAAGACTGCTAAACTCTAACACTTACTCATCTGCCTATCCACTCCATGATGGCCAATA TTGGAAGCCATCAGAACCTCCCAATCCTACCAATGAAAGATACACACTTCACCAGAATTGGGCTCGATTT TCCTATTTCTACAAGGAGCAGCCTTTAGACTTGATTAAGAATTATTATGGAGAAAAAATTGGTATCTATT TTGTCTTTCTTGGATTTTACACAGAAATGCTATTCTTTGCAGCTGTAGTTGGCTTAGCTTGTTTTATTTA TGGCTTATTATCAATGGAACATAACACAAGCAGCACTGAAATCTGTGACCCTGAGATTGGTGGTCAGATG ATCATGTGCCCACTCTGTGATCAAGTGTGTGATTATTGGAGACTAAATAGTACGTGTTTGGCTTCAAAGT TCTCCCATTTGTTTGATAATGAGTCAACAGTGTTCTTTGCAATATTCATGGGAATTTGGGTCACCTTATT TTTGGAGTTTTGGAAACAACGACAAGCCAGACTGGAATATGAATGGGACCTGGTGGACTTTGAAGAGGAA CAGCAGCAGCTTCAGCTGAGACCAGAATTTGAAGCTATGTGTAAACACAGGAAATTGAATGCAGTGACTA AGGAGATGGAACCTTACATGCCTCTATACACGCGTATTCCATGGTACTTTCTTTCAGGAGCCACAGTGAC ATTATGGATGTCTCTTGTCGTCACCAGTATGGTAGCTGTAATTGTGTACCGCCTGTCAGTCTTTGCTACA TTTGCTAGTTTCATGGAAAGTGATGCATCCTTAAAGCAGGTCAAAAGCTTCCTTACTCCTCAGATAACCA CATCACTCACAGGATCATGCTTGAACTTTATTGTCATCTTGATCTTGAATTTCTTTTATGAAAAGATATC TGCCTGGATCACAAAAATGGAAATTCCTCGAACATACCAGGAGTATGAGAGCAGTCTTACCTTGAAAATG TTCCTGTTTCAGTTTGTAAATTTTTACTCATCCTGCTTCTACGTAGCTTTCTTTAAAGGGAAGTTCGTAG GCTATCCTGGAAAATACACATATTTATTTAATGAGTGGAGAAGTGAAGAGTGTGATCCTGGAGGCTGTCT TATAGAATTGACAACCCAATTGACCATTATAATGACCGGGAAACAGATTTTTGGAAACATTAAAGAAGCC ATTTATCCCTTGGCTTTGAATTGGTGGAGACGCCGAAAAGCTCGGACAAACTCTGAGAAGCTGTATAGTC GATGGGAGCAGGATCATGACCTTGAAAGTTTTGGACCCCTTGGGCTTTTCTATGAGTACTTAGAAACAGT TACTCAATTTGGATTTGTTACACTATTTGTGGCCTCTTTTCCTTTGGCTCCTCTTCTTGCTCTCATAAAT AATATTGTAGAGATTCGAGTGGATGCCTGGAAACTTACCACTCAATACAGGAGAACTGTAGCTTCTAAAG CTCATAGCATAGGTGTTTGGCAAGACATTCTTTATGGAATGGCTGTCCTTTCTGTTGCAACTAATGCCTT TATTGTTGCATTTACGTCAGACATCATTCCCCGTCTAGTTTACTACTATGCTTACTCAACAAATGCCACA CAGCCTATGACAGGATATGTGAATAATAGCCTGTCAGTATTCCTGATAGCTGATTTTCCAAACCACACTG CACCTTCGGAAAAACGAGACTTCATCACTTGCAGGTACAGAGATTACAGATATCCTCCTGATGACGAGAA TAAATATTTTCATAATATGCAATTCTGGCATGTCCTTGCTGCCAAGATGACCTTCATCATTGTTATGGAA CATGTTGTGTTTTTAGTTAAATTTTTGCTGGCCTGGATGATACCTGATGTTCCAAAAGATGTTGTGGAGA GAATCAAGAGAGAAAAGTTAATGACTATCAAGATTCTCCATGATTTTGAGCTCAACAAATTAAAAGAGAA CTTGGGAATTAATTCTAATGAATTTGCCAAGCATGTCATGATTGAGGAAAACAAAGCACAGCTGGCTAAA TCAACACTC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC224062 representing NM_213599
Red=Cloning site Green=Tags(s) MGDPDLLEVLAEEGEKVNKHIDYSFQMSEQSLSSRETSFLINEETMPAKRFNLFLRRRLMFQKNQQSKDS IFFRDGIRQIDFVLSYVDDVKKDAELKAERRKEFETNLRKTGLELEIEDKRDSEDGRTYFVKIHAPWEVL VTYAEVLGIKMPIKESDIPRPKHTPISYVLGPVRLPLSVKYPHPEYFTAQFSRHRQELFLIEDQATFFPS SSRNRIVYYILSRCPFGIEDGKKRFGIERLLNSNTYSSAYPLHDGQYWKPSEPPNPTNERYTLHQNWARF SYFYKEQPLDLIKNYYGEKIGIYFVFLGFYTEMLFFAAVVGLACFIYGLLSMEHNTSSTEICDPEIGGQM IMCPLCDQVCDYWRLNSTCLASKFSHLFDNESTVFFAIFMGIWVTLFLEFWKQRQARLEYEWDLVDFEEE QQQLQLRPEFEAMCKHRKLNAVTKEMEPYMPLYTRIPWYFLSGATVTLWMSLVVTSMVAVIVYRLSVFAT FASFMESDASLKQVKSFLTPQITTSLTGSCLNFIVILILNFFYEKISAWITKMEIPRTYQEYESSLTLKM FLFQFVNFYSSCFYVAFFKGKFVGYPGKYTYLFNEWRSEECDPGGCLIELTTQLTIIMTGKQIFGNIKEA IYPLALNWWRRRKARTNSEKLYSRWEQDHDLESFGPLGLFYEYLETVTQFGFVTLFVASFPLAPLLALIN NIVEIRVDAWKLTTQYRRTVASKAHSIGVWQDILYGMAVLSVATNAFIVAFTSDIIPRLVYYYAYSTNAT QPMTGYVNNSLSVFLIADFPNHTAPSEKRDFITCRYRDYRYPPDDENKYFHNMQFWHVLAAKMTFIIVME HVVFLVKFLLAWMIPDVPKDVVERIKREKLMTIKILHDFELNKLKENLGINSNEFAKHVMIEENKAQLAK STL SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_213599 |
| ORF Size | 2739 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_213599.3 |
| RefSeq Size | 3147 bp |
| RefSeq ORF | 2742 bp |
| Locus ID | 203859 |
| UniProt ID | Q75V66 |
| Protein Families | Transmembrane |
| MW | 107 kDa |
| Gene Summary | This gene encodes a member of the anoctamin family of transmembrane proteins. The encoded protein is likely a calcium activated chloride channel. Mutations in this gene have been associated with gnathodiaphyseal dysplasia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224062L3 | Lenti ORF clone of Human anoctamin 5 (ANO5), transcript variant 1, Myc-DDK-tagged |
CNY 12,432.00 |
|
| RC224062L4 | Lenti ORF clone of Human anoctamin 5 (ANO5), transcript variant 1, mGFP tagged |
CNY 11,500.00 |
|
| RG224062 | ANO5 (tGFP-tagged) - Human anoctamin 5 (ANO5), transcript variant 1 |
CNY 11,632.00 |
|
| SC314907 | ANO5 (untagged)-Human anoctamin 5 (ANO5), transcript variant 1 |
CNY 17,580.00 |
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