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BRIP1 (NM_032043) Human Tagged ORF Clone
CAT＃: RC224085
BRIP1 (Myc-DDK-tagged)-Human BRCA1 interacting protein C-terminal helicase 1 (BRIP1)
ORF Plasmid: tGFP
"NM_032043" in other vectors (6)
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CNY 8,696.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BACH1; FANCJ; OF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224085 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTCAATGTGGTCTGAATATACAATTGGTGGGGTGAAGATTTACTTTCCTTATAAAGCTTACCCGT CACAGCTTGCTATGATGAATTCTATTCTCAGAGGATTAAACAGCAAGCAACATTGTTTGTTGGAGAGTCC CACAGGAAGTGGAAAAAGCTTAGCCTTACTTTGTTCTGCTTTAGCATGGCAACAATCTCTTAGTGGGAAA CCAGCAGATGAGGGCGTAAGTGAAAAAGCTGAAGTACAATTGTCATGTTGTTGTGCATGCCATTCAAAGG ATTTTACAAACAATGACATGAACCAAGGAACTTCACGTCATTTCAACTATCCAAGCACACCACCTTCTGA AAGAAATGGCACTTCATCAACTTGTCAAGACTCCCCTGAAAAAACCACTCTGGCTGCAAAGTTATCTGCT AAGAAACAGGCATCCATATACAGAGATGAAAATGATGATTTTCAAGTAGAGAAGAAAAGAATTCGACCCT TAGAAACTACACAGCAGATTAGAAAACGTCATTGCTTTGGAACAGAAGTACACAATTTGGATGCAAAAGT TGATTCAGGAAAGACTGTAAAACTCAACTCTCCACTGGAAAAGATAAACTCCTTTTCGCCACAGAAACCC CCTGGCCACTGTTCTAGGTGCTGTTGTTCTACTAAACAAGGAAACAGTCAAGAGTCATCGAATACCATTA AGAAGGATCATACAGGGAAATCCAAGATACCCAAAATATATTTTGGGACACGCACACACAAGCAGATTGC TCAGATTACTAGAGAGCTCCGGAGGACGGCATATTCAGGGGTTCCAATGACTATTCTTTCCAGCAGGGAT CATACTTGTGTCCATCCTGAGGTAGTCGGTAACTTCAACAGAAATGAGAAGTGCATGGAATTGCTAGATG GGAAAAACGGAAAATCCTGCTATTTTTATCATGGAGTTCATAAAATTAGTGATCAGCACACATTACAGAC TTTCCAAGGGATGTGCAAAGCCTGGGATATAGAAGAACTTGTCAGCCTGGGGAAGAAACTAAAGGCCTGT CCATATTACACAGCCCGAGAACTAATACAAGATGCTGACATCATATTTTGTCCCTACAACTATCTTCTAG ATGCACAAATAAGGGAAAGTATGGATTTAAATCTGAAAGAACAGGTTGTCATTTTAGATGAAGCTCATAA CATCGAGGACTGTGCTCGGGAATCAGCAAGTTACAGTGTAACAGAAGTTCAGCTTCGGTTTGCTCGGGAT GAACTAGATAGTATGGTCAACAATAATATAAGGAAGAAAGATCATGAACCCCTACGAGCTGTGTGCTGTA GCCTCATTAATTGGTTAGAAGCAAACGCTGAATATCTTGTAGAAAGAGATTATGAATCAGCTTGTAAAAT ATGGAGTGGAAATGAAATGCTCTTAACTTTACACAAAATGGGTATCACCACTGCTACTTTTCCCATTTTG CAGGGACATTTTTCTGCTGTTCTTCAAAAAGAGGAAAAAATCTCACCAATTTATGGTAAAGAGGAGGCAA GAGAAGTACCTGTTATTAGTGCATCAACTCAAATAATGCTTAAAGGACTTTTTATGGTACTTGACTATCT TTTTAGGCAAAATAGCAGATTTGCAGATGATTATAAAATTGCGATTCAACAGACTTACTCCTGGACAAAT CAGATTGATATTTCAGACAAAAATGGGTTGTTGGTTCTACCAAAAAATAAGAAACGTTCACGACAGAAAA CTGCAGTTCATGTGCTAAACTTTTGGTGCTTAAATCCAGCTGTGGCCTTTTCAGATATTAATGGCAAAGT TCAGACCATTGTTTTGACATCTGGTACATTATCACCAATGAAATCCTTTTCGTCAGAACTTGGTGTTACA TTTACTATCCAGCTGGAGGCTAATCATATCATTAAAAATTCACAGGTTTGGGTTGGTACCATTGGGTCAG GCCCCAAGGGTCGGAATCTCTGTGCTACCTTCCAGAATACTGAAACATTTGAGTTCCAAGATGAAGTGGG AGCACTTTTGTTATCTGTGTGCCAGACTGTGAGCCAAGGAATTTTGTGTTTCTTGCCATCTTACAAGTTA TTAGAAAAATTAAAAGAACGTTGGCTCTCTACTGGTTTATGGCATAATCTGGAGTTGGTGAAGACAGTCA TTGTAGAACCACAGGGAGGAGAAAAAACAAATTTTGATGAATTACTGCAGGTGTACTATGACGCAATCAA ATACAAAGGAGAGAAAGATGGAGCTCTCCTGGTAGCAGTTTGTCGTGGTAAAGTGAGTGAGGGTCTGGAT TTCTCAGATGACAATGCCCGTGCTGTCATAACAATAGGAATTCCTTTTCCAAATGTGAAAGATCTACAGG TTGAACTAAAACGACAATACAATGACCACCATTCAAAATTGAGAGGTCTTCTACCTGGCCGTCAGTGGTA TGAAATTCAAGCATACAGGGCCTTAAACCAGGCCCTTGGTAGATGTATTAGACACAGAAATGATTGGGGA GCTCTTATTCTAGTGGATGATCGCTTTAGGAATAACCCAAGTCGCTATATATCTGGACTTTCTAAATGGG TACGGCAGCAGATTCAGCACCATTCAACCTTTGAAAGTGCACTGGAGTCCTTGGCTGAATTTTCCAAAAA GCATCAAAAAGTTCTTAATGTATCCATAAAGGACAGAACCAATATACAGGACAATGAGTCTACACTTGAA GTGACCTCTTTAAAGTACAGTACCCCACCTTATTTACTGGAAGCAGCAAGTCATCTATCACCAGAAAATT TTGTGGAAGATGAAGCAAAGATATGTGTCCAGGAACTACAGTGTCCTAAAATTATTACCAAAAATTCACC TCTACCAAGTAGCATTATCTCCAGAAAGGAGAAAAATGATCCAGTATTCCTGGAAGAAGCAGGGAAAGCA GAAAAAATTGTGATTTCCAGATCCACAAGCCCAACTTTCAACAAACAAACAAAGAGAGTTAGCTGGTCAA GCTTTAATTCTTTGGGACAGTATTTTACTGGTAAAATACCGAAGGCAACACCTGAGCTCGGGTCATCAGA GAATAGTGCCTCTAGTCCTCCCCGTTTCAAAACAGAGAAGATGGAAAGTAAAACTGTTTTGCCCTTCACT GATAAATGTGAATCCTCAAATCTGACAGTAAACACATCGTTTGGATCATGCCCTCAATCAGAAACCATTA TTTCATCATTAAAGATTGATGCCACCCTTACTAGAAAAAATCATTCTGAACATCCGCTCTGTTCTGAAGA AGCCCTGGATCCAGACATTGAATTGTCTCTAGTAAGTGAAGAAGATAAACAGTCCACTTCAAATAGAGAT TTTGAAACAGAAGCAGAAGATGAATCTATCTATTTTACACCTGAACTTTACGATCCTGAAGATACAGATG AAGAAAAAAATGACCTAGCTGAAACTGATAGAGGAAATAGATTGGCTAACAATTCAGATTGCATTTTAGC TAAAGACCTTTTTGAAATTAGAACTATAAAAGAAGTAGATTCAGCCAGAGAAGTGAAAGCTGAGGATTGC ATAGATACAAAGTTGAATGGAATTCTGCATATTGAAGAAAGTAAAATTGATGACATTGATGGTAATGTAA AAACAACTTGGATAAATGAACTGGAACTGGGAAAAACTCATGAAATAGAAATAAAGAACTTTAAACCATC TCCTTCCAAAAATAAAGGCATGTTTCCTGGTTTTAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224085 protein sequence
Red=Cloning site Green=Tags(s) MSSMWSEYTIGGVKIYFPYKAYPSQLAMMNSILRGLNSKQHCLLESPTGSGKSLALLCSALAWQQSLSGK PADEGVSEKAEVQLSCCCACHSKDFTNNDMNQGTSRHFNYPSTPPSERNGTSSTCQDSPEKTTLAAKLSA KKQASIYRDENDDFQVEKKRIRPLETTQQIRKRHCFGTEVHNLDAKVDSGKTVKLNSPLEKINSFSPQKP PGHCSRCCCSTKQGNSQESSNTIKKDHTGKSKIPKIYFGTRTHKQIAQITRELRRTAYSGVPMTILSSRD HTCVHPEVVGNFNRNEKCMELLDGKNGKSCYFYHGVHKISDQHTLQTFQGMCKAWDIEELVSLGKKLKAC PYYTARELIQDADIIFCPYNYLLDAQIRESMDLNLKEQVVILDEAHNIEDCARESASYSVTEVQLRFARD ELDSMVNNNIRKKDHEPLRAVCCSLINWLEANAEYLVERDYESACKIWSGNEMLLTLHKMGITTATFPIL QGHFSAVLQKEEKISPIYGKEEAREVPVISASTQIMLKGLFMVLDYLFRQNSRFADDYKIAIQQTYSWTN QIDISDKNGLLVLPKNKKRSRQKTAVHVLNFWCLNPAVAFSDINGKVQTIVLTSGTLSPMKSFSSELGVT FTIQLEANHIIKNSQVWVGTIGSGPKGRNLCATFQNTETFEFQDEVGALLLSVCQTVSQGILCFLPSYKL LEKLKERWLSTGLWHNLELVKTVIVEPQGGEKTNFDELLQVYYDAIKYKGEKDGALLVAVCRGKVSEGLD FSDDNARAVITIGIPFPNVKDLQVELKRQYNDHHSKLRGLLPGRQWYEIQAYRALNQALGRCIRHRNDWG ALILVDDRFRNNPSRYISGLSKWVRQQIQHHSTFESALESLAEFSKKHQKVLNVSIKDRTNIQDNESTLE VTSLKYSTPPYLLEAASHLSPENFVEDEAKICVQELQCPKIITKNSPLPSSIISRKEKNDPVFLEEAGKA EKIVISRSTSPTFNKQTKRVSWSSFNSLGQYFTGKIPKATPELGSSENSASSPPRFKTEKMESKTVLPFT DKCESSNLTVNTSFGSCPQSETIISSLKIDATLTRKNHSEHPLCSEEALDPDIELSLVSEEDKQSTSNRD FETEAEDESIYFTPELYDPEDTDEEKNDLAETDRGNRLANNSDCILAKDLFEIRTIKEVDSAREVKAEDC IDTKLNGILHIEESKIDDIDGNVKTTWINELELGKTHEIEIKNFKPSPSKNKGMFPGFK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032043 |
| ORF Size | 3747 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032043.1, NP_114432.1 |
| RefSeq Size | 8166 bp |
| RefSeq ORF | 3750 bp |
| Locus ID | 83990 |
| UniProt ID | Q9BX63 |
| Protein Families | Druggable Genome |
| MW | 140.9 kDa |
| Gene Summary | The protein encoded by this gene is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). This gene may be a target of germline cancer-inducing mutations. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Identification of a BRCA1-mRNA Splicing Complex Required for Efficient DNA Repair and Maintenance of Genomic Stability
,Savage, KI;Gorski, JJ;Barros, EM;Irwin, GW;Manti, L;Powell, AJ;Pellagatti, A;Lukashchuk, N;McCance, DJ;McCluggage, WG;Schettino, G;Salto-Tellez, M;Boultwood, J;Richard, DJ;McDade, SS;Harkin, DP;,
Mol. Cell. April 2014
,PubMed ID 24746700
[BRIP1]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224085L1 | Lenti ORF clone of Human BRCA1 interacting protein C-terminal helicase 1 (BRIP1), Myc-DDK-tagged |
CNY 11,096.00 |
|
| RC224085L2 | Lenti ORF clone of Human BRCA1 interacting protein C-terminal helicase 1 (BRIP1), mGFP tagged |
CNY 14,350.00 |
|
| RC224085L3 | Lenti ORF clone of Human BRCA1 interacting protein C-terminal helicase 1 (BRIP1), Myc-DDK-tagged |
CNY 14,350.00 |
|
| RC224085L4 | Lenti ORF clone of Human BRCA1 interacting protein C-terminal helicase 1 (BRIP1), mGFP tagged |
CNY 11,096.00 |
|
| RG224085 | BRIP1 (tGFP-tagged) - Human BRCA1 interacting protein C-terminal helicase 1 (BRIP1) |
CNY 13,680.00 |
|
| SC305391 | BRIP1 (untagged)-Human BRCA1 interacting protein C-terminal helicase 1 (BRIP1) |
CNY 10,450.00 |
