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POU6F2 (NM_007252) Human Tagged ORF Clone
CAT＃: RC224192
- TrueORF®
POU6F2 (Myc-DDK-tagged)-Human POU class 6 homeobox 2 (POU6F2), transcript variant 1
ORF Plasmid: tGFP
"NM_007252" in other vectors (6)
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CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RPF-1; WT5; WTSL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224192 representing NM_007252
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGCTCTTCTTCAGGATCCAATGATAGCTGGACAAGTCAGTAAGCCCTTGCTGTCAGTGCGGAGTG AAATGAATGCGGAGTTGAGAGGTGAGGACAAGGCTGCTACTTCAGACAGCGAGCTGAATGAGCCCCTGCT TGCGCCTGTGGAATCAAATGACAGCGAGGACACTCCCAGCAAGCTCTTCGGGGCTAGAGGAAACCCAGCA TTATCAGACCCAGGCACTCCTGACCAACACCAGGCCAGTCAGACCCACCCCCCATTTCCAGTTGGGCCAC AGCCACTTCTGACGGCACAGCAGTTAGCTTCTGCTGTGGCCGGCGTGATGCCGGGAGGCCCCCCAGCCCT CAACCAGCCAATCCTCATTCCCTTCAACATGGCGGGACAGCTAGGAGGCCAGCAAGGACTGGTTCTCACA CTGCCAACAGCGAATCTCACCAACATCCAAGGGCTGGTGGCAGCAGCTGCAGCCGGAGGCATTATGACTC TGCCACTGCAAAATCTACAAGCTACCTCATCCCTGAACTCCCAGCTCCAGCAGCTCCAGCTCCAGCTCCA GCAGCAGCAGCAGCAGCAGCAGCAGCAGCCTCCCCCGTCAACCAACCAGCACCCGCAACCAGCCCCACAG GCGCCCTCGCAGTCCCAGCAGCAGCCGCTGCAGCCCACCCCACCCCAGCAGCCACCACCCGCCTCTCAGC AGCCGCCAGCTCCTACATCTCAGCTGCAACAGGCGCCTCAGCCCCAGCAGCACCAACCCCACTCCCACTC CCAGAACCAGAACCAACCATCTCCAACCCAGCAGAGCTCCAGCCCCCCGCAGAAACCTAGTCAGTCTCCA GGACATGGCCTGCCTTCACCGCTCACGCCACCCAATCCTCTACAGCTGGTTAATAATCCACTAGCAAGTC AGGCTGCAGCGGCTGCAGCAGCCATGAGCTCCATAGCAAGCTCACAGGCCTTTGGCAATGCCCTCTCCAG TCTTCAGGGGGTCACAGGTCAACTAGTTACTAATGCACAAGGACAGATTATCGGGACCATTCCACTGATG CCTAATCCAGGGCCATCGAGCCAAGCAGCAAGCGGCACTCAGGGCTTGCAAGTGCAGCCAATCACCCCCC AGCTCCTCACAAACGCCCAGGGCCAGATCATCGCCACAGTCATTGGGAACCAGATCCTGCCCGTGATCAA CACCCAGGGCATCACGCTGTCACCCATCAAGCCCGGCCAGCAGCTCCACCAACCCTCCCAGACGTCAGTG GGTCAAGCAGCCTCCCAAGGCAACCTTCTGCACCTGGCTCACAGCCAAGCATCCATGTCTCAAAGTCCCG TCCGGCAGGCTTCCTCTTCTTCCTCCTCATCCTCCTCTTCTTCAGCTTTGAGCGTGGGCCAGTTAGTCAG CAATCCTCAAACGGCAGCGGGTGAGGTGGATGGGGTTAATCTGGAGGAGATCCGAGAATTTGCCAAAGCT TTTAAAATCCGGCGCCTGTCCCTTGGCCTGACCCAGACTCAGGTGGGACAGGCTCTCAGTGCTACAGAGG GCCCCGCGTACAGCCAGTCGGCCATCTGCAGACACACCATCCTGAGAAGCCACTTTTTCCTACCACAGGA AGCCCAAGAGAACACTATAGCTAGCAGTCTGACAGCCAAACTGAACCCTGGCCTTTTGTATCCTGCCAGG TTTGAAAAGCTGGACATCACCCCTAAAAGTGCCCAGAAGATCAAGCCGGTGCTTGAGCGGTGGATGGCTG AGGCTGAGGCCCGCCATCGAGCAGGTATGCAGAACCTGACCGAGTTTATCGGGAGTGAACCATCCAAAAA GCGCAAGCGGCGCACCTCCTTCACACCCCAGGCCCTTGAGATCCTCAATGCCCACTTTGAGAAGAACACA CACCCTTCTGGGCAGGAAATGACCGAAATTGCTGAGAAGCTGAACTATGACCGAGAAGTAGTTAGAGTTT GGTTCTGCAATAAGAGGCAAGCCCTGAAGAACACAATTAAACGCTTAAAACAGCACGAGCCGGCCACGGC AGTCCCTTTGGAGCCCTTAACAGACTCTCTGGAAGAAAACTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224192 representing NM_007252
Red=Cloning site Green=Tags(s) MSALLQDPMIAGQVSKPLLSVRSEMNAELRGEDKAATSDSELNEPLLAPVESNDSEDTPSKLFGARGNPA LSDPGTPDQHQASQTHPPFPVGPQPLLTAQQLASAVAGVMPGGPPALNQPILIPFNMAGQLGGQQGLVLT LPTANLTNIQGLVAAAAAGGIMTLPLQNLQATSSLNSQLQQLQLQLQQQQQQQQQQPPPSTNQHPQPAPQ APSQSQQQPLQPTPPQQPPPASQQPPAPTSQLQQAPQPQQHQPHSHSQNQNQPSPTQQSSSPPQKPSQSP GHGLPSPLTPPNPLQLVNNPLASQAAAAAAAMSSIASSQAFGNALSSLQGVTGQLVTNAQGQIIGTIPLM PNPGPSSQAASGTQGLQVQPITPQLLTNAQGQIIATVIGNQILPVINTQGITLSPIKPGQQLHQPSQTSV GQAASQGNLLHLAHSQASMSQSPVRQASSSSSSSSSSSALSVGQLVSNPQTAAGEVDGVNLEEIREFAKA FKIRRLSLGLTQTQVGQALSATEGPAYSQSAICRHTILRSHFFLPQEAQENTIASSLTAKLNPGLLYPAR FEKLDITPKSAQKIKPVLERWMAEAEARHRAGMQNLTEFIGSEPSKKRKRRTSFTPQALEILNAHFEKNT HPSGQEMTEIAEKLNYDREVVRVWFCNKRQALKNTIKRLKQHEPATAVPLEPLTDSLEENS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007252 |
| ORF Size | 2073 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007252.4 |
| RefSeq Size | 2324 bp |
| RefSeq ORF | 2076 bp |
| Locus ID | 11281 |
| UniProt ID | P78424 |
| Domains | POU, homeobox |
| Protein Families | Transcription Factors |
| MW | 73.3 kDa |
| Gene Summary | This gene encodes a member of the POU protein family characterized by the presence of a bipartite DNA binding domain, consisting of a POU-specific domain and a homeodomain, separated by a variable polylinker. The DNA binding domain may bind to DNA as monomers or as homo- and/or heterodimers, in a sequence-specific manner. The POU family members are transcriptional regulators, many of which are known to control cell type-specific differentiation pathways. This gene is a tumor suppressor involved in Wilms tumor (WT) predisposition. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Oct 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224192L1 | Lenti-ORF clone of POU6F2 (Myc-DDK-tagged)-Human POU class 6 homeobox 2 (POU6F2), transcript variant 1 |
CNY 7,700.00 |
|
| RC224192L2 | Lenti-ORF clone of POU6F2 (mGFP-tagged)-Human POU class 6 homeobox 2 (POU6F2), transcript variant 1 |
CNY 9,992.00 |
|
| RC224192L3 | Lenti-ORF clone of POU6F2 (Myc-DDK-tagged)-Human POU class 6 homeobox 2 (POU6F2), transcript variant 1 |
CNY 7,700.00 |
|
| RC224192L4 | Lenti-ORF clone of POU6F2 (mGFP-tagged)-Human POU class 6 homeobox 2 (POU6F2), transcript variant 1 |
CNY 7,700.00 |
|
| RG224192 | POU6F2 (tGFP-tagged) - Human POU class 6 homeobox 2 (POU6F2), transcript variant 1 |
CNY 6,370.00 |
|
| SC115616 | POU6F2 (untagged)-Human POU class 6 homeobox 2 (POU6F2), transcript variant 1 |
CNY 5,072.00 |
