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PRMT1 (NM_001536) Human Tagged ORF Clone
CAT＃: RC224239
PRMT1 (Myc-DDK-tagged)-Human protein arginine methyltransferase 1 (PRMT1), transcript variant 1
ORF Plasmid: tGFP
"NM_001536" in other vectors (7)
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CNY 3,656.00
CNY 4,370.00
| Cited in 8 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ANM1; HCP1; HRMT1L2; IR1B4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224239 representing NM_001536
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCAGCCGAGGCCGCGAACTGCATCATGGAGAATTTTGTAGCCACCTTGGCTAATGGGATGAGCC TCCAGCCGCCTCTTGAAGAAGTGTCCTGTGGCCAGGCGGAAAGCAGTGAGAAGCCCAACGCTGAGGACAT GACATCCAAAGATTACTACTTTGACTCCTACGCACACTTTGGCATCCACGAGGAGATGCTGAAGGACGAG GTGCGCACCCTCACTTACCGCAACTCCATGTTTCATAACCGGCACCTCTTCAAGGACAAGGTGGTGCTGG ACGTCGGCTCGGGCACCGGCATCCTCTGCATGTTTGCTGCCAAGGCCGGGGCCCGCAAGGTCATCGGGAT CGAGTGTTCCAGTATCTCTGATTATGCGGTGAAGATCGTCAAAGCCAACAAGTTAGACCACGTGGTGACC ATCATCAAGGGGAAGGTGGAGGAGGTGGAGCTCCCAGTGGAGAAGGTGGACATCATCATCAGCGAGTGGA TGGGCTACTGCCTCTTCTACGAGTCCATGCTCAACACCGTGCTCTATGCCCGGGACAAGTGGCTGGCGCC CGATGGCCTCATCTTCCCAGACCGGGCCACGCTGTATGTGACGGCCATCGAGGACCGGCAGTACAAAGAC TACAAGATCCACTGGTGGGAGAACGTGTATGGCTTCGACATGTCTTGCATCAAAGATGTGGCCATTAAGG AGCCCCTAGTGGATGTCGTGGACCCCAAACAGCTGGTCACCAACGCCTGCCTCATAAAGGAGGTGGACAT CTATACCGTCAAGGTGGAAGACCTGACCTTCACCTCCCCGTTCTGCCTGCAAGTGAAGCGGAATGACTAC GTGCACGCCCTGGTGGCCTACTTCAACATCGAGTTCACACGCTGCCACAAGAGGACCGGCTTCTCCACCA GCCCCGAGTCCCCGTACACGCACTGGAAGCAGACGGTGTTCTACATGGAGGACTACCTGACCGTGAAGAC GGGCGAGGAGATCTTCGGCACCATCGGCATGCGGCCCAACGCCAAGAACAACCGGGACCTGGACTTCACC ATCGACCTGGACTTCAAGGGCCAGCTGTGCGAGCTGTCCTGCTCCACCGACTACCGGATGCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224239 representing NM_001536
Red=Cloning site Green=Tags(s) MAAAEAANCIMENFVATLANGMSLQPPLEEVSCGQAESSEKPNAEDMTSKDYYFDSYAHFGIHEEMLKDE VRTLTYRNSMFHNRHLFKDKVVLDVGSGTGILCMFAAKAGARKVIGIECSSISDYAVKIVKANKLDHVVT IIKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVLYARDKWLAPDGLIFPDRATLYVTAIEDRQYKD YKIHWWENVYGFDMSCIKDVAIKEPLVDVVDPKQLVTNACLIKEVDIYTVKVEDLTFTSPFCLQVKRNDY VHALVAYFNIEFTRCHKRTGFSTSPESPYTHWKQTVFYMEDYLTVKTGEEIFGTIGMRPNAKNNRDLDFT IDLDFKGQLCELSCSTDYRMR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001536 |
| ORF Size | 1113 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001536.6 |
| RefSeq Size | 1386 bp |
| RefSeq ORF | 1116 bp |
| Locus ID | 3276 |
| UniProt ID | Q99873 |
| MW | 42.3 kDa |
| Gene Summary | This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011] |
Citations (8)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
PRMT1-dependent methylation of BRCA1 contributes to the epigenetic defense of breast cancer cells against ionizing radiation
,null,
Scientific Reports
,PubMed ID 32764667
[PRMT1]
|
|
The Protein Arginine Methyltransferases 1 and 5 affect Myc properties in glioblastoma stem cells
,Favia, A;Salvatori, L;Nanni, S;Iwamoto-Stohl, LK;Valente, S;Mai, A;Scagnoli, F;Fontanella, RA;Totta, P;Nasi, S;Illi, B;,
Sci Rep
,PubMed ID 31685892
[PRMT1]
|
|
Hepatocyte PRMT1 protects from alcohol induced liver injury by modulating oxidative stress responses
,Zhao, J;Adams, A;Weinman, SA;Tikhanovich, I;,
Sci Rep
,PubMed ID 31235809
[PRMT1]
|
|
Arginine methylation is required for canonical Wnt signaling and endolysosomal trafficking
,Albrecht, LV;Ploper, D;Tejeda-Muñoz, N;De Robertis, EM;,
Proc. Natl. Acad. Sci. U.S.A.
,PubMed ID 29773710
[PRMT1]
|
|
PRMT1 and JMJD6 dependent arginine methylation regulate HNF4α expression and hepatocyte proliferation
,Zhao, J;Adams, A;Roberts, B;O'Neil, M;Vittal, A;Schmitt, T;Kumer, S;Cox, J;Li, Z;Weinman, SA;Tikhanovich, I;,
Hepatology
,PubMed ID 29023917
[PRMT1]
|
|
PRMT5: A novel regulator of Hepatitis B virus replication and an arginine methylase of HBV core
,Lubyova, B;Hodek, J;Zabransky, A;Prouzova, H;Hubalek, M;Hirsch, I;Weber, J;,
PLoS ONE
,PubMed ID 29065155
[PRMT1]
|
|
Arginine methylation regulates c-Myc-dependent transcription by altering promoter recruitment of the acetyltransferase p300
,Tikhanovich, I;Zhao, J;Bridges, B;Kumer, S;Roberts, B;Weinman, SA;,
J. Biol. Chem.
,PubMed ID 28652407
[PRMT1]
|
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Dynamic arginine methylation of TNF receptor associated factor 6 regulates Toll-like receptor signaling
,Tikhanovich, I;Kuravi, S;Artigues, A;Villar, MT;Dorko, K;Nawabi, A;Roberts, B;Weinman, SA;,
J. Biol. Chem.
,PubMed ID 26221041
[PRMT1]
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Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224239L1 | Lenti ORF clone of Human protein arginine methyltransferase 1 (PRMT1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC224239L2 | Lenti ORF clone of Human protein arginine methyltransferase 1 (PRMT1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC224239L3 | Lenti ORF clone of Human protein arginine methyltransferase 1 (PRMT1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC224239L4 | Lenti ORF clone of Human protein arginine methyltransferase 1 (PRMT1), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG224239 | PRMT1 (tGFP-tagged) - Human protein arginine methyltransferase 1 (PRMT1), transcript variant 1 |
CNY 5,256.00 |
|
| SC309036 | PRMT1 (untagged)-Human protein arginine methyltransferase 1 (PRMT1), transcript variant 1 |
CNY 6,080.00 |
|
| SC317596 | PRMT1 (untagged)-Human protein arginine methyltransferase 1 (PRMT1), transcript variant 1 |
CNY 3,656.00 |
