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ROBO2 (NM_002942) Human Tagged ORF Clone
CAT＃: RC224282
- TrueORF®
ROBO2 (Myc-DDK-tagged)-Human roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant 2
ORF Plasmid: tGFP
"NM_002942" in other vectors (4)
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CNY 13,780.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SAX3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224282 representing NM_002942
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCTGCTGATGTTTACACAACTACTGCTCTGTGGATTTTTATATGTTCGGGTTGATGGATCGCGTC TTCGCCAGGAGGACTTTCCCCCGCGGATTGTGGAGCATCCTTCCGATGTCATCGTCTCTAAGGGCGAGCC CACGACTCTGAACTGCAAGGCGGAGGGCCGGCCAACGCCCACCATTGAGTGGTACAAAGATGGGGAGCGA GTGGAGACTGACAAGGACGATCCCCGGTCCCACAGGATGCTTCTGCCCAGCGGATCCTTATTCTTCTTGC GCATCGTGCACGGGCGCAGGAGTAAACCTGATGAAGGAAGCTACGTTTGTGTTGCGAGGAACTATCTTGG TGAAGCAGTGAGTCGAAATGCGTCTCTGGAAGTGGCATTGTTACGAGATGACTTCCGACAAAACCCCACA GATGTTGTAGTGGCAGCTGGAGAGCCTGCAATCCTGGAGTGCCAGCCTCCCCGGGGACACCCAGAACCCA CCATCTACTGGAAAAAAGACAAAGTTCGAATTGATGACAAGGAAGAAAGAATAAGTATCCGTGGTGGAAA ACTGATGATCTCCAATACCAGGAAAAGTGATGCAGGGATGTATACTTGTGTTGGTACCAATATGGTGGGA GAAAGGGACAGTGACCCAGCAGAGCTGACTGTCTTTGAACGACCCACATTTCTCAGGAGGCCAATTAACC AGGTGGTACTGGAGGAAGAAGCTGTAGAATTTCGTTGTCAAGTCCAAGGAGATCCTCAACCAACTGTGAG GTGGAAAAAGGATGATGCAGACTTGCCAAGAGGAAGGTATGACATCAAAGACGATTACACACTAAGAATT AAAAAGACCATGAGTACAGATGAAGGCACCTATATGTGTATTGCTGAGAATCGGGTTGGAAAAATGGAAG CCTCTGCTACACTCACCGTCCGAGCTCCCCCACAGTTTGTGGTTCGGCCAAGAGATCAGATTGTTGCTCA AGGTCGAACAGTGACATTTCCCTGTGAAACTAAAGGAAACCCACAGCCAGCTGTTTTTTGGCAGAAAGAA GGCAGCCAGAACCTACTTTTCCCAAACCAACCCCAGCAGCCCAACAGTAGATGCTCAGTGTCACCAACTG GAGACCTCACAATCACCAACATTCAACGTTCCGACGCGGGTTACTACATCTGCCAGGCTTTAACTGTGGC AGGAAGCATTTTAGCAAAAGCTCAACTGGAGGTTACTGATGTTTTGACAGATAGACCTCCACCTATAATT CTACAAGGCCCAGCCAACCAAACGCTGGCAGTGGATGGTACAGCGTTACTGAAATGTAAAGCCACTGGTG ATCCTCTTCCTGTAATTAGCTGGTTAAAGGAGGGATTTACTTTTCCGGGTAGAGATCCAAGAGCAACAAT TCAAGAGCAAGGCACACTGCAGATTAAGAATTTACGGATTTCTGATACTGGCACTTATACTTGTGTGGCT ACAAGTTCAAGTGGAGAGACTTCCTGGAGTGCAGTGCTGGATGTGACAGAGTCTGGAGCAACAATCAGTA AAAACTATGATTTAAGTGACCTGCCAGGGCCACCATCCAAACCGCAGGTCACTGATGTTACTAAGAACAG TGTCACCTTGTCCTGGCAGCCAGGTACCCCTGGAACCCTTCCAGCAAGTGCATATATCATTGAGGCTTTC AGCCAATCAGTGAGCAACAGCTGGCAGACCGTGGCAAACCATGTAAAGACCACCCTCTATACTGTAAGAG GACTGCGGCCCAATACAATCTACTTATTCATGGTCAGAGCGATCAACCCCCAAGGTCTCAGTGACCCAAG TCCCATGTCAGATCCTGTGCGCACACAAGATATCAGCCCACCAGCACAAGGAGTGGACCACAGGCAAGTG CAGAAAGAGCTAGGAGATGTCCTTGTCCGTCTTCATAATCCAGTTGTGCTGACTCCCACCACGGTTCAGG TCACATGGACGGTTGATCGCCAACCCCAGTTTATCCAAGGCTACCGAGTGATGTATCGTCAGACTTCAGG TCTGCAGGCGACATCTTCGTGGCAGAATTTAGATGCCAAAGTCCCGACTGAACGAAGTGCTGTCTTAGTC AACCTGAAAAAGGGGGTGACTTATGAAATTAAAGTACGGCCATATTTTAATGAGTTCCAAGGAATGGATA GTGAATCTAAAACGGTTCGTACTACTGAAGAAGCCCCAAGTGCCCCACCACAGTCTGTCACTGTACTGAC AGTTGGAAGCTACAATAGCACAAGTATTAGTGTTTCCTGGGATCCTCCTCCTCCAGATCACCAGAATGGA ATTATCCAAGAATACAAGATCTGGTGTCTAGGAAATGAAACGCGATTCCATATCAACAAAACTGTGGATG CAGCCATTCGGTCCGTAATAATTGGTGGATTATTCCCAGGTATTCAATACCGGGTAGAGGTTGCAGCTAG TACCAGTGCAGGGGTTGGAGTAAAGAGTGAGCCACAGCCAATAATAATCGGGAGACGCAATGAAGTTGTC ATTACTGAAAACAATAACAGCATAACTGAGCAAATCACTGATGTGGTGAAGCAACCAGCCTTTATAGCTG GTATTGGTGGTGCCTGCTGGGTAATTCTGATGGGTTTTAGCATATGGTTGTATTGGCGAAGAAAGAAGAG GAAGGGACTCAGTAATTATGCTGTTACGTTTCAAAGAGGAGATGGAGGACTAATGAGCAATGGAAGCCGT CCAGGTCTTCTCAATGCTGGTGATCCCAGCTATCCATGGCTTGCTGATTCTTGGCCAGCCACGAGCTTGC CAGTAAATAATAGCAACAGTGGCCCAAATGAGATTGGAAATTTTGGCCGTGGAGATGTGCTGCCACCAGT TCCAGGCCAAGGGGATAAAACAGCAACGATGCTCTCAGATGGAGCCATTTATAGTAGCATTGACTTCACT ACCAAAACCAGTTACAACAGTTCCAGCCAAATAACACAGGCTACCCCATATGCCACGACACAGATCTTGC ATTCCAACAGCATACATGAATTGGCTGTCGATCTGCCTGATCCACAATGGAAAAGCTCAATTCAGCAAAA AACAGATCTGATGGGATTTGGTTATTCTCTACCTGATCAGAACAAAGGTAACAATGGTGGGAAAGGTGGA AAAAAGAAGAAAAATAAAAACTCTTCTAAACCACAGAAAAACAATGGATCCACTTGGGCCAATGTCCCTC TACCTCCCCCCCCAGTCCAGCCCCTTCCTGGCACGGAGCTGGAACACTATGCAGTGGAACAACAAGAAAA TGGCTATGACAGTGATAGCTGGTGCCCACCATTGCCAGTACAAACTTACTTACACCAAGGTCTGGAAGAT GAACTGGAAGAAGATGATGATAGGGTCCCAACACCTCCTGTTCGAGGCGTGGCTTCTTCTCCTGCTATCT CCTTTGGACAGCAGTCCACTGCAACTCTTACTCCATCCCCACGGGAAGAGATGCAACCCATGCTGCAGGC TCACCTGGATGAGTTGACAAGAGCCTATCAGTTTGATATAGCAAAACAAACATGGCACATTCAAAGCAAT AATCAACCTCCACAGCCTCCAGTTCCACCGTTAGGTTATGTGTCTGGAGCCTTGATTTCTGATTTGGAAA CGGATGTTGCAGATGATGATGCCGACGACGAAGAGGAAGCTTTAGAAATCCCCAGGCCCCTGAGAGCACT GGACCAGACTCCTGGATCCAGCATGGACAATCTAGACAGCTCTGTGACAGGAAAAGCCTTTACCTCCTCT CAAAGACCTCGACCTACCAGCCCATTTTCTACTGACAGTAACACCAGTGCAGCCCTGAGTCAAAGTCAGA GGCCTCGGCCCACTAAAAAACACAAGGGAGGGCGGATGGACCAACAACCAGCATTGCCTCATCGAAGGGA AGGAATGACAGATGAGGAGGCCTTGGTGCCCTATAGCAAGCCCAGTTTCCCATCTCCAGGTGGCCACAGC TCATCAGGAACAGCTTCTTCTAAGGGATCCACTGGACCTAGGAAAACCGAGGTGTTGAGAGCAGGCCACC AGCGCAATGCCAGCGACCTTCTTGACATAGGATATATGGGCTCCAACAGTCAAGGACAGTTTACAGGTGA ATTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224282 representing NM_002942
Red=Cloning site Green=Tags(s) MSLLMFTQLLLCGFLYVRVDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGER VETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPT DVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVG ERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRI KKTMSTDEGTYMCIAENRVGKMEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKE GSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPII LQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVA TSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAF SQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQV QKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLV NLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNG IIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVV ITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSR PGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFT TKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNGGKGG KKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLED ELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSN NQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSS QRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHS SSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002942 |
| ORF Size | 4134 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002942.5 |
| RefSeq Size | 8689 bp |
| RefSeq ORF | 4137 bp |
| Locus ID | 6092 |
| UniProt ID | Q9HCK4 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Axon guidance |
| MW | 151.2 kDa |
| Gene Summary | The protein encoded by this gene belongs to the ROBO family, part of the immunoglobulin superfamily of proteins that are highly conserved from fly to human. The encoded protein is a transmembrane receptor for the slit homolog 2 protein and functions in axon guidance and cell migration. Mutations in this gene are associated with vesicoureteral reflux, characterized by the backward flow of urine from the bladder into the ureters or the kidney. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224282L3 | Lenti-ORF clone of ROBO2 (Myc-DDK-tagged)-Human roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant 2 |
CNY 15,680.00 |
|
| RC224282L4 | Lenti-ORF clone of ROBO2 (mGFP-tagged)-Human roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant 2 |
CNY 15,680.00 |
|
| RG224282 | ROBO2 (tGFP-tagged) - Human roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant 2 |
CNY 15,200.00 |
|
| SC303253 | ROBO2 (untagged)-Human roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant 2 |
CNY 26,510.00 |
