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AMPD3 (NM_001025390) Human Tagged ORF Clone
CAT＃: RC224327
AMPD3 (Myc-DDK-tagged)-Human adenosine monophosphate deaminase 3 (AMPD3), transcript variant 3
ORF Plasmid: tGFP
"NM_001025390" in other vectors (4)
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CNY 5,672.00
CNY 8,170.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224327 representing NM_001025390
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCAGGCTCAGCTGAGATGCCGCGGCAGTTTCCCAAGCTGAACATCTCTGAAGTGGATGAGCAAG TCCGGCTCCTGGCGGAGAAGGTGTTTGCTAAAGTGCTCCGAGAAGAGGACAGCAAAGATGCCCTGTCCCT GTTCACTGTCCCAGAGGACTGCCCCATCGGGCAAAAGGAAGCCAAGGAGAGGGAGCTGCAGAAGGAGCTG GCAGAGCAGAAGTCTGTGGAGACCGCAAAAAGAAAGAAAAGTTTCAAGATGATTCGGTCCCAGTCCCTGT CTCTGCAAATGCCGCCACAGCAAGATTGGAAGGGCCCCCCGGCAGCCAGTCCGGCCATGTCTCCCACAAC CCCTGTGGTCACTGGAGCCACTTCCCTGCCCACGCCAGCACCCTATGCCATGCCTGAGTTCCAGCGGGTC ACCATCAGCGGAGATTACTGTGCCGGGATCACTTTGGAGGACTATGAGCAGGCAGCCAAGAGTCTGGCCA AGGCCCTAATGATCCGGGAGAAGTATGCGCGGCTCGCCTACCACCGCTTCCCGCGGATCACATCCCAGTA CCTGGGTCATCCGCGGGCGGATACTGCACCTCCGGAAGAGGGCCTTCCAGACTTCCACCCTCCTCCACTG CCCCAGGAAGACCCCTACTGCCTGGATGATGCACCCCCCAACCTGGATTACTTGGTCCACATGCAGGGGG GCATCCTCTTTGTGTATGATAACAAGAAGATGCTGGAGCACCAGGAGCCGCACAGCCTACCCTACCCCGA CCTGGAGACCTACACGGTGGACATGAGCCACATCCTGGCTCTCATCACCGATGGCCCCACGAAAACCTAT TGTCACCGGCGACTGAACTTTCTGGAATCCAAGTTCAGCCTTCATGAGATGTTAAACGAAATGTCCGAGT TCAAAGAGTTGAAGAGTAACCCCCACCGGGACTTCTATAACGTGAGAAAGGTGGACACACACATCCATGC GGCCGCCTGCATGAACCAAAAGCATCTGCTGCGCTTCATCAAGCACACATACCAGACGGAGCCTGACAGG ACTGTGGCAGAGAAGCGGGGCCGGAAGATCACCCTGCGGCAGGTGTTTGACGGCCTGCACATGGACCCCT ACGACCTCACTGTGGACTCACTGGATGTCCACGCGGGCCGGCAGACATTCCACCGCTTTGACAAGTTCAA CTCCAAATACAACCCTGTGGGGGCCAGTGAGCTGCGTGACCTGTATTTGAAAACTGAAAACTATCTGGGA GGAGAGTACTTTGCTCGGATGGTCAAGGAGGTTGCCCGGGAGCTGGAGGAGAGCAAGTACCAGTACTCAG AGCCACGGCTCTCCATCTACGGCCGCAGTCCTGAGGAGTGGCCCAACCTGGCCTACTGGTTCATCCAGCA CAAGGTCTACTCTCCCAACATGCGCTGGATCATCCAGGTGCCCCGGATTTATGACATATTTAGGTCAAAG AAGCTGCTGCCAAACTTTGGGAAGATGCTGGAGAACATCTTCCTGCCCCTTTTCAAGGCCACTATCAACC CCCAAGATCATCGAGAGCTTCACCTCTTCCTTAAATATGTGACGGGGTTTGACAGCGTGGATGATGAGTC CAAGCACAGCGACCACATGTTTTCCGACAAGAGCCCAAACCCGGACGTCTGGACCAGTGAGCAGAACCCA CCCTACAGCTACTACCTGTACTACATGTATGCCAACATCATGGTGCTCAACAACCTCCGCAGGGAGCGCG GCCTGAGCACGTTCCTGTTCCGGCCGCACTGTGGGGAAGCCGGCTCCATCACCCACCTGGTGTCTGCCTT CCTCACTGCTGACAACATTTCCCACGGGCTGCTCCTCAAGAAGAGTCCGGTATTGCAGTATCTCTACTAC CTTGCTCAGATCCCCATTGCCATGTCTCCTCTTAGCAACAACAGTTTGTTCCTCGAATATTCCAAGAACC CTCTGAGGGAATTCCTACACAAGGGACTGCATGTTTCTCTTTCCACCGATGACCCCATGCAGTTCCACTA CACGAAGGAAGCACTTATGGAAGAATATGCCATTGCAGCTCAAGTGTGGAAGCTGAGCACCTGCGACCTG TGTGAGATCGCCAGGAACAGCGTGCTGCAGAGCGGCCTCTCGCATCAGGAAAAGCAAAAGTTTCTGGGAC AAAATTATTATAAAGAAGGACCTGAAGGAAATGATATTCGAAAGACAAATGTGGCTCAGATCCGGATGGC ATTCCGATATGAGACCTTATGCAATGAGCTCAGCTTCCTGTCTGATGCTATGAAATCAGAAGAGATCACC GCCTTGACCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224327 representing NM_001025390
Red=Cloning site Green=Tags(s) MEPGSAEMPRQFPKLNISEVDEQVRLLAEKVFAKVLREEDSKDALSLFTVPEDCPIGQKEAKERELQKEL AEQKSVETAKRKKSFKMIRSQSLSLQMPPQQDWKGPPAASPAMSPTTPVVTGATSLPTPAPYAMPEFQRV TISGDYCAGITLEDYEQAAKSLAKALMIREKYARLAYHRFPRITSQYLGHPRADTAPPEEGLPDFHPPPL PQEDPYCLDDAPPNLDYLVHMQGGILFVYDNKKMLEHQEPHSLPYPDLETYTVDMSHILALITDGPTKTY CHRRLNFLESKFSLHEMLNEMSEFKELKSNPHRDFYNVRKVDTHIHAAACMNQKHLLRFIKHTYQTEPDR TVAEKRGRKITLRQVFDGLHMDPYDLTVDSLDVHAGRQTFHRFDKFNSKYNPVGASELRDLYLKTENYLG GEYFARMVKEVARELEESKYQYSEPRLSIYGRSPEEWPNLAYWFIQHKVYSPNMRWIIQVPRIYDIFRSK KLLPNFGKMLENIFLPLFKATINPQDHRELHLFLKYVTGFDSVDDESKHSDHMFSDKSPNPDVWTSEQNP PYSYYLYYMYANIMVLNNLRRERGLSTFLFRPHCGEAGSITHLVSAFLTADNISHGLLLKKSPVLQYLYY LAQIPIAMSPLSNNSLFLEYSKNPLREFLHKGLHVSLSTDDPMQFHYTKEALMEEYAIAAQVWKLSTCDL CEIARNSVLQSGLSHQEKQKFLGQNYYKEGPEGNDIRKTNVAQIRMAFRYETLCNELSFLSDAMKSEEIT ALTN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001025390 |
| ORF Size | 2322 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001025390.2 |
| RefSeq Size | 4473 bp |
| RefSeq ORF | 2325 bp |
| Locus ID | 272 |
| UniProt ID | Q01432 |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Purine metabolism |
| MW | 89.3 kDa |
| Gene Summary | This gene encodes a member of the AMP deaminase gene family. The encoded protein is a highly regulated enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate to inosine monophosphate, a branch point in the adenylate catabolic pathway. This gene encodes the erythrocyte (E) isoforms, whereas other family members encode isoforms that predominate in muscle (M) and liver (L) cells. Mutations in this gene lead to the clinically asymptomatic, autosomal recessive condition erythrocyte AMP deaminase deficiency. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224327L3 | Lenti ORF clone of Human adenosine monophosphate deaminase 3 (AMPD3), transcript variant 3, Myc-DDK-tagged |
CNY 10,070.00 |
|
| RC224327L4 | Lenti ORF clone of Human adenosine monophosphate deaminase 3 (AMPD3), transcript variant 3, mGFP tagged |
CNY 10,070.00 |
|
| RG224327 | AMPD3 (tGFP-tagged) - Human adenosine monophosphate deaminase 3 (AMPD3), transcript variant 3 |
CNY 9,030.00 |
|
| SC302401 | AMPD3 (untagged)-Human adenosine monophosphate deaminase 3 (AMPD3), transcript variant 3 |
CNY 15,010.00 |
