DIAPH2 (NM_007309) Human Tagged ORF Clone
CAT#: RC224466
- TrueORF®
DIAPH2 (Myc-DDK-tagged)-Human diaphanous homolog 2 (Drosophila) (DIAPH2), transcript variant 12C
ORF Plasmid: tGFP
"NM_007309" in other vectors (4)
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CNY 10,930.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | DIA; DIA2; DRF2; POF; POF2; POF2A |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC224466 representing NM_007309
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCAGCCCGGGGCGGCGGCGTCGGGAGCGGGAGGCGGCAGCGAGGAACCCGGTGGGGGCCGGAGCA ACAAGCGGAGCGCGGGGAACCGGGCCGCCAATGAAGAGGAAACGAAAAACAAACCCAAATTGAACATTCA AATAAAAACTTTGGCAGATGATGTGCGTGACCGAATTACAAGTTTTAGAAAATCTACTGTCAAAAAAGAA AAACCTCTTATTCAACATCCTATTGATTCTCAAGTCGCGATGAGTGAGTTTCCTGCAGCTCAGCCATTAT ATGATGAACGATCTTTGAATTTGTCAGAAAAGGAAGTATTGGATCTCTTTGAAAAAATGATGGAGGACAT GAACCTTAACGAAGAGAAAAAAGCTCCTTTACGAAACAAAGACTTTACCACCAAACGTGAGATGGTTGTC CAGTATATTTCTGCCACTGCCAAATCTGGTGGGCTGAAAAACAGCAAACATGAATGCACCCTGTCTTCAC AAGAATATGTTCATGAATTACGATCGGGTATATCAGATGAGAAACTTCTTAATTGCCTAGAATCCCTCAG GGTTTCTTTAACCAGCAATCCGGTCAGCTGGGTTAACAACTTTGGCCATGAAGGTCTTGGACTCTTATTG GATGAGCTGGAAAAGCTTCTGGACAAAAAACAGCAAGAAAATATTGACAAGAAGAATCAGTATAAACTTA TTCAATGCCTCAAAGCATTTATGAATAATAAGTTTGGATTACAAAGGATTCTAGGAGATGAAAGAAGTCT TTTACTATTGGCAAGAGCAATTGACCCCAAACAACCCAACATGATGACTGAAATAGTAAAAATACTTTCT GCTATTTGCATTGTTGGAGAAGAGAACATTCTAGATAAACTTTTAGGGGCTATAACAACAGCAGCAGAAA GAAATAACAGGGAACGATTTTCACCAATTGTGGAAGGTTTAGAAAATCAGGAAGCCTTGCAATTACAGGT GGCCTGCATGCAGTTTATAAATGCCCTTGTCACTTCTCCTTATGAGCTTGATTTTCGAATACATTTAAGG AATGAATTCCTCCGTTCAGGACTAAAAACAATGTTACCAGATCTAAAAGAAAAAGAGAATGATGAGCTTG ATATTCAGTTGAAAGTATTTGATGAAAACAAAGAAGATGACCTAACTGAATTATCACACCGTCTCAATGA CATTCGAGCAGAAATGGATGATATGAATGAAGTCTACCATCTTCTATATAATATGCTGAAGGACACTGCT GCTGAAAATTACTTCTTATCTATTCTACAACATTTTTTGCTTATCAGAAATGATTATTATATCAGGCCAC AATATTATAAAATAATTGAGGAATGTGTTTCACAGATAGTGCTACACTGCAGTGGTATGGATCCAGACTT CAAATACAGGCAAAGATTAGACATCGATTTAACTCATCTGATAGATTCTTGTGTGAACAAGGCGAAAGTT GAAGAAAGTGAACAAAAAGCTGCAGAGTTTTCAAAGAAGTTCGATGAAGAATTCACAGCTCGACAGGAAG CTCAAGCAGAGCTTCAAAAAAGAGATGAGAAAATCAAAGAACTTGAAGCAGAAATCCAGCAACTTCGAAC CCAGGCACAAGTACTCTCAAGTTCATCAGGAATTCCAGGTCCTCCTGCAGCACCTCCATTGCCAGGTGTA GGGCCGCCTCCACCACCACCCGCGCCACCTCTACCCGGAGGAGCTCCTCTTCCTCCTCCACCACCTCCTT TACCTGGAATGATGGGGATACCACCACCACCCCCACCACCACTTTTATTTGGGGGACCTCCTCCACCACC ACCCCTTGGAGGAGTTCCTCCTCCCCCAGGAATATCACTTAATCTACCTTATGGAATGAAGCAGAAAAAA ATGTATAAACCTGAAGTGTCCATGAAGAGAATCAATTGGTCAAAGATTGAACCCACAGAATTATCTGAGA ACTGTTTCTGGTTAAGAGTCAAAGAAGACAAGTTTGAGAATCCAGATCTCTTTGCCAAATTGGCATTGAA TTTTGCTACTCAGATAAAAGTTCAAAAGAACGCAGAAGCATTAGAAGAAAAGAAGACTGGGCCTACAAAG AAGAAAGTGAAAGAACTGAGAATTTTGGATCCCAAAACAGCTCAGAATCTGTCCATCTTTCTGGGATCAT ATCGCATGCCATATGAAGACATAAGAAACGTTATTCTGGAGGTTAATGAAGACATGCTGAGTGAGGCTTT AATTCAGAACCTTGTGAAACATCTTCCTGAGCAGAAGATACTCAACGAATTAGCAGAGCTTAAGAATGAA TATGATGACCTCTGTGAGCCTGAACAATTTGGAGTTGTGATGAGCTCTGTGAAAATGTTACAGCCTCGTC TCAGTAGTATCCTGTTCAAGCTCACATTTGAAGAACACATAAACAACATCAAACCAAGCATCATAGCAGT AACTCTTGCCTGTGAAGAACTGAAGAAAAGTGAAAGCTTTAACAGACTTTTAGAGTTAGTTCTTCTTGTT GGAAACTACATGAACTCAGGCTCAAGAAATGCCCAGTCTTTGGGATTTAAGATCAACTTCCTTTGTAAGA TCAGAGATACTAAATCAGCGGATCAAAAAACAACCCTTTTGCATTTTATTGCCGACATTTGTGAGGAAAA ATATCGAGATATCCTAAAATTTCCTGAAGAACTGGAACACGTAGAAAGTGCAAGCAAAGTTTCAGCTCAA ATTCTCAAGAGCAACCTTGCATCAATGGAACAACAAATTGTTCATCTGGAACGTGACATCAAGAAATTCC CCCAAGCAGAAAATCAACACGATAAGTTTGTGGAAAAGATGACCAGCTTTACAAAGACTGCCCGAGAACA GTATGAAAAACTCTCCACCATGCACAACAACATGATGAAGCTCTATGAGAATCTTGGAGAATACTTCATT TTTGACTCAAAGACAGTGAGCATAGAAGAGTTCTTTGGTGATCTCAACAACTTCCGAACTTTGTTTTTGG AAGCAGTGAGAGAAAACAATAAGAGAAGAGAAATGGAAGAGAAGACCAGGAGGGCAAAACTTGCAAAAGA GAAAGCTGAACAAGAAAAGTTAGAACGCCAGAAGAAAAAGAAACAACTCATTGATATAAACAAAGAGGGT GATGAGACTGGTGTGATGGATAATCTTCTAGAAGCCCTACAATCAGGTGCAGCATTCAGAGACCGTCGAA AGCGGATTCCAAGGAATCCAGTGGTAAATCATCCCTGTGCAACAAGGGCTAATCCAAGATCAGCTACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224466 representing NM_007309
Red=Cloning site Green=Tags(s) MEQPGAAASGAGGGSEEPGGGRSNKRSAGNRAANEEETKNKPKLNIQIKTLADDVRDRITSFRKSTVKKE KPLIQHPIDSQVAMSEFPAAQPLYDERSLNLSEKEVLDLFEKMMEDMNLNEEKKAPLRNKDFTTKREMVV QYISATAKSGGLKNSKHECTLSSQEYVHELRSGISDEKLLNCLESLRVSLTSNPVSWVNNFGHEGLGLLL DELEKLLDKKQQENIDKKNQYKLIQCLKAFMNNKFGLQRILGDERSLLLLARAIDPKQPNMMTEIVKILS AICIVGEENILDKLLGAITTAAERNNRERFSPIVEGLENQEALQLQVACMQFINALVTSPYELDFRIHLR NEFLRSGLKTMLPDLKEKENDELDIQLKVFDENKEDDLTELSHRLNDIRAEMDDMNEVYHLLYNMLKDTA AENYFLSILQHFLLIRNDYYIRPQYYKIIEECVSQIVLHCSGMDPDFKYRQRLDIDLTHLIDSCVNKAKV EESEQKAAEFSKKFDEEFTARQEAQAELQKRDEKIKELEAEIQQLRTQAQVLSSSSGIPGPPAAPPLPGV GPPPPPPAPPLPGGAPLPPPPPPLPGMMGIPPPPPPPLLFGGPPPPPPLGGVPPPPGISLNLPYGMKQKK MYKPEVSMKRINWSKIEPTELSENCFWLRVKEDKFENPDLFAKLALNFATQIKVQKNAEALEEKKTGPTK KKVKELRILDPKTAQNLSIFLGSYRMPYEDIRNVILEVNEDMLSEALIQNLVKHLPEQKILNELAELKNE YDDLCEPEQFGVVMSSVKMLQPRLSSILFKLTFEEHINNIKPSIIAVTLACEELKKSESFNRLLELVLLV GNYMNSGSRNAQSLGFKINFLCKIRDTKSADQKTTLLHFIADICEEKYRDILKFPEELEHVESASKVSAQ ILKSNLASMEQQIVHLERDIKKFPQAENQHDKFVEKMTSFTKTAREQYEKLSTMHNNMMKLYENLGEYFI FDSKTVSIEEFFGDLNNFRTLFLEAVRENNKRREMEEKTRRAKLAKEKAEQEKLERQKKKKQLIDINKEG DETGVMDNLLEALQSGAAFRDRRKRIPRNPVVNHPCATRANPRSAT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007309 |
ORF Size | 3288 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007309.3, NP_009293.1 |
RefSeq Size | 3782 bp |
RefSeq ORF | 3291 bp |
Locus ID | 1730 |
UniProt ID | O60879 |
Domains | FH2 |
Protein Families | Druggable Genome |
Protein Pathways | Regulation of actin cytoskeleton |
MW | 124.8 kDa |
Gene Summary | The product of this gene belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this gene have been linked to premature ovarian failure 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC224466L3 | Lenti-ORF clone of DIAPH2 (Myc-DDK-tagged)-Human diaphanous homolog 2 (Drosophila) (DIAPH2), transcript variant 12C |
CNY 12,830.00 |
|
RC224466L4 | Lenti-ORF clone of DIAPH2 (mGFP-tagged)-Human diaphanous homolog 2 (Drosophila) (DIAPH2), transcript variant 12C |
CNY 12,830.00 |
|
RG224466 | DIAPH2 (tGFP-tagged) - Human diaphanous homolog 2 (Drosophila) (DIAPH2), transcript variant 12C |
CNY 11,970.00 |
|
SC315175 | DIAPH2 (untagged)-Human diaphanous homolog 2 (Drosophila) (DIAPH2), transcript variant 12C |
CNY 21,000.00 |