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LPP (NM_005578) Human Tagged ORF Clone
CAT＃: RC224539
LPP (Myc-DDK-tagged)-Human LIM domain containing preferred translocation partner in lipoma (LPP), transcript variant 1
ORF Plasmid: tGFP
"NM_005578" in other vectors (4)
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CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224539 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCACCCATCTTGGCTGCCACCCAAAAGCACTGGTGAGCCCCTCGGCCATGTGCCTGCACGGATGG AGACCACCCATTCCTTTGGGAACCCCAGCATTTCAGTGTCTACACAACAGCCACCCAAAAAGTTTGCCCC GGTAGTTGCTCCAAAACCTAAGTACAACCCATACAAACAACCTGGAGGTGAGGGTGATTTTCTTCCACCC CCACCTCCACCTCTAGATGATTCCAGTGCCCTTCCATCTATCTCTGGAAACTTTCCTCCTCCACCACCTC TTGATGAAGAGGCTTTCAAAGTACAGGGGAATCCCGGAGGCAAGACACTTGAGGAGAGGCGCTCCAGCCT GGACGCTGAGATTGACTCCTTGACCAGCATCTTGGCTGACCTTGAGTGCAGCTCCCCCTATAAGCCTCGG CCTCCACAGAGCTCCACTGGTTCAACAGCCTCTCCTCCAGTTTCGACCCCAGTCACAGGACACAAGAGAA TGGTCATCCCGAACCAACCCCCTCTAACAGCAACCAAGAAGTCTACATTGAAACCACAGCCTGCACCCCA GGCTGGACCCATCCCTGTGGCTCCAATCGGAACACTCAAACCCCAGCCTCAGCCAGTCCCAGCCTCCTAC ACCACGGCCTCCACTTCTTCAAGGCCTACCTTTAATGTGCAGGTGAAGTCAGCCCAGCCCAGCCCTCATT ATATGGCTGCCCCTTCATCAGGACAAATTTATGGCTCAGGGCCCCAGGGCTATAACACTCAGCCAGTTCC TGTCTCTGGGCAGTGTCCACCTCCTTCAACACGGGGAGGCATGGATTATGCCTACATTCCACCACCAGGA CTTCAGCCGGAGCCTGGGTATGGGTATGCCCCCAACCAGGGACGCTATTATGAAGGCTACTATGCAGCAG GGCCAGGCTATGGGGGCAGAAATGACTCTGATCCTACCTATGGTCAACAAGGTCACCCAAATACCTGGAA ACGGGAACCAGGGTACACTCCTCCTGGAGCAGGGAACCAGAACCCTCCTGGGATGTATCCAGTCACTGGT CCCAAGAAGACCTATATCACAGATCCTGTTTCAGCCCCCTGTGCGCCACCATTGCAGCCAAAGGGTGGCC ATTCAGGGCAACTGGGGCCTTCGTCAGTTGCCCCTTCATTCCGCCCAGAGGATGAGCTTGAGCACCTGAC CAAAAAGATGCTGTATGACATGGAAAATCCACCTGCTGACGAATACTTTGGCCGCTGTGCTCGCTGTGGA GAAAACGTAGTTGGGGAAGGTACAGGATGCACTGCCATGGATCAGGTCTTCCACGTGGATTGTTTTACCT GCATCATCTGCAACAACAAGCTCCGAGGGCAGCCATTCTATGCTGTGGAAAAGAAAGCATACTGCGAGCC CTGCTACATTAATACTCTGGAGCAGTGCAATGTGTGTTCCAAGCCCATCATGGAGCGGATTCTCCGAGCC ACCGGGAAGGCCTATCATCCTCACTGTTTCACCTGCGTGATGTGCCACCGCAGCCTGGATGGGATCCCAT TCACTGTGGATGCTGGCGGGCTCATTCACTGCATTGAGGACTTCCACAAGAAATTTGCCCCGCGGTGTTC TGTGTGCAAGGAGCCTATTATGCCAGCCCCGGGCCAGGAGGAGACTGTCCGTATTGTGGCTTTGGATCGA GATTTCCATGTTCACTGCTACCGATGCGAGGATTGCGGTGGTCTCCTGTCTGAAGGAGATAACCAAGGCT GCTACCCCTTGGATGGGCACATCCTCTGCAAGACCTGCAACTCTGCCCGCATCAGGGTGTTGACCGCCAA GGCGAGCACTGACCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224539 protein sequence
Red=Cloning site Green=Tags(s) MSHPSWLPPKSTGEPLGHVPARMETTHSFGNPSISVSTQQPPKKFAPVVAPKPKYNPYKQPGGEGDFLPP PPPPLDDSSALPSISGNFPPPPPLDEEAFKVQGNPGGKTLEERRSSLDAEIDSLTSILADLECSSPYKPR PPQSSTGSTASPPVSTPVTGHKRMVIPNQPPLTATKKSTLKPQPAPQAGPIPVAPIGTLKPQPQPVPASY TTASTSSRPTFNVQVKSAQPSPHYMAAPSSGQIYGSGPQGYNTQPVPVSGQCPPPSTRGGMDYAYIPPPG LQPEPGYGYAPNQGRYYEGYYAAGPGYGGRNDSDPTYGQQGHPNTWKREPGYTPPGAGNQNPPGMYPVTG PKKTYITDPVSAPCAPPLQPKGGHSGQLGPSSVAPSFRPEDELEHLTKKMLYDMENPPADEYFGRCARCG ENVVGEGTGCTAMDQVFHVDCFTCIICNNKLRGQPFYAVEKKAYCEPCYINTLEQCNVCSKPIMERILRA TGKAYHPHCFTCVMCHRSLDGIPFTVDAGGLIHCIEDFHKKFAPRCSVCKEPIMPAPGQEETVRIVALDR DFHVHCYRCEDCGGLLSEGDNQGCYPLDGHILCKTCNSARIRVLTAKASTDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005578 |
| ORF Size | 1836 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005578.5 |
| RefSeq Size | 18296 bp |
| RefSeq ORF | 1839 bp |
| Locus ID | 4026 |
| UniProt ID | Q93052 |
| Domains | LIM |
| MW | 65.7 kDa |
| Gene Summary | This gene encodes a member of a subfamily of LIM domain proteins that are characterized by an N-terminal proline-rich region and three C-terminal LIM domains. The encoded protein localizes to the cell periphery in focal adhesions and may be involved in cell-cell adhesion and cell motility. This protein also shuttles through the nucleus and may function as a transcriptional co-activator. This gene is located at the junction of certain disease-related chromosomal translocations, which result in the expression of chimeric proteins that may promote tumor growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224539L3 | Lenti ORF clone of Human LIM domain containing preferred translocation partner in lipoma (LPP), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC224539L4 | Lenti ORF clone of Human LIM domain containing preferred translocation partner in lipoma (LPP), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG224539 | LPP (tGFP-tagged) - Human LIM domain containing preferred translocation partner in lipoma (LPP), transcript variant 1 |
CNY 6,160.00 |
|
| SC116667 | LPP (untagged)-Human LIM domain containing preferred translocation partner in lipoma (LPP), transcript variant 1 |
CNY 4,568.00 |
