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DYRK3 (NM_003582) Human Tagged ORF Clone
CAT＃: RC224654
- TrueORF®
DYRK3 (Myc-DDK-tagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (DYRK3), transcript variant 1
ORF Plasmid: tGFP
"NM_003582" in other vectors (6)
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CNY 6,568.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DYRK5; hYAK3-2; RED; REDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224654 representing NM_003582
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGGCACAGCTCGTGGGCCTGGGCGGAAGGATGCGGGGCCGCCTGGGGCCGGGCTCCCGCCCCAGC AGCGGAGGTTGGGGGATGGTGTCTATGACACCTTCATGATGATAGATGAAACCAAATGTCCCCCCTGTTC AAATGTACTCTGCAATCCTTCTGAACCACCTCCACCCAGAAGACTAAATATGACCACTGAGCAGTTTACA GGAGATCATACTCAGCACTTTTTGGATGGAGGTGAGATGAAGGTAGAACAGCTGTTTCAAGAATTTGGCA ACAGAAAATCCAATACTATTCAGTCAGATGGCATCAGTGACTCTGAAAAATGCTCTCCTACTGTTTCTCA GGGTAAAAGTTCAGATTGCTTGAATACAGTAAAATCCAACAGTTCATCCAAGGCACCCAAAGTGGTGCCT CTGACTCCAGAACAAGCCCTGAAGCAATATAAACACCACCTCACTGCCTATGAGAAACTGGAAATAATTA ATTATCCAGAAATTTACTTTGTAGGTCCAAATGCCAAGAAAAGACATGGAGTTATTGGTGGTCCCAATAA TGGAGGGTATGATGATGCAGATGGGGCCTATATTCATGTACCTCGAGACCATCTAGCTTATCGATATGAG GTGCTGAAAATTATTGGCAAGGGGAGTTTTGGGCAGGTGGCCAGGGTCTATGATCACAAACTTCGACAGT ACGTGGCCCTAAAAATGGTGCGCAATGAGAAGCGCTTTCATCGTCAAGCAGCTGAGGAGATCCGGATTTT GGAGCATCTTAAGAAACAGGATAAAACTGGTAGTATGAACGTTATCCACATGCTGGAAAGTTTCACATTC CGGAACCATGTTTGCATGGCCTTTGAATTGCTGAGCATAGACCTTTATGAGCTGATTAAAAAAAATAAGT TTCAGGGTTTTAGCGTCCAGTTGGTACGCAAGTTTGCCCAGTCCATCTTGCAATCTTTGGATGCCCTCCA CAAAAATAAGATTATTCACTGCGATCTGAAGCCAGAAAACATTCTCCTGAAACACCACGGGCGCAGTTCA ACCAAGGTCATTGACTTTGGGTCCAGCTGTTTCGAGTACCAGAAGCTCTACACATATATCCAGTCTCGGT TCTACAGAGCTCCAGAAATCATCTTAGGAAGCCGCTACAGCACACCAATTGACATATGGAGTTTTGGCTG CATCCTTGCAGAACTTTTAACAGGACAGCCTCTCTTCCCTGGAGAGGATGAAGGAGACCAGTTGGCCTGC ATGATGGAGCTTCTAGGGATGCCACCACCAAAACTTCTGGAGCAATCCAAACGTGCCAAGTACTTTATTA ATTCCAAGGGCATACCCCGCTACTGCTCTGTGACTACCCAGGCAGATGGGAGGGTTGTGCTTGTGGGGGG TCGCTCACGTAGGGGTAAAAAGCGGGGTCCCCCAGGCAGCAAAGACTGGGGGACAGCACTGAAAGGGTGT GATGACTACTTGTTTATAGAGTTCTTGAAAAGGTGTCTTCACTGGGACCCCTCTGCCCGCTTGACCCCAG CTCAAGCATTAAGACACCCTTGGATTAGCAAGTCTGTCCCCAGACCTCTCACCACCATAGACAAGGTGTC AGGGAAACGGGTAGTTAATCCTGCAAGTGCTTTCCAGGGATTGGGTTCTAAGCTGCCTCCAGTTGTTGGA ATAGCCAATAAGCTTAAAGCTAACTTAATGTCAGAAACCAATGGTAGTATACCCCTATGCAGTGTATTGC CAAAACTGATTAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224654 representing NM_003582
Red=Cloning site Green=Tags(s) MGGTARGPGRKDAGPPGAGLPPQQRRLGDGVYDTFMMIDETKCPPCSNVLCNPSEPPPPRRLNMTTEQFT GDHTQHFLDGGEMKVEQLFQEFGNRKSNTIQSDGISDSEKCSPTVSQGKSSDCLNTVKSNSSSKAPKVVP LTPEQALKQYKHHLTAYEKLEIINYPEIYFVGPNAKKRHGVIGGPNNGGYDDADGAYIHVPRDHLAYRYE VLKIIGKGSFGQVARVYDHKLRQYVALKMVRNEKRFHRQAAEEIRILEHLKKQDKTGSMNVIHMLESFTF RNHVCMAFELLSIDLYELIKKNKFQGFSVQLVRKFAQSILQSLDALHKNKIIHCDLKPENILLKHHGRSS TKVIDFGSSCFEYQKLYTYIQSRFYRAPEIILGSRYSTPIDIWSFGCILAELLTGQPLFPGEDEGDQLAC MMELLGMPPPKLLEQSKRAKYFINSKGIPRYCSVTTQADGRVVLVGGRSRRGKKRGPPGSKDWGTALKGC DDYLFIEFLKRCLHWDPSARLTPAQALRHPWISKSVPRPLTTIDKVSGKRVVNPASAFQGLGSKLPPVVG IANKLKANLMSETNGSIPLCSVLPKLIS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003582 |
| ORF Size | 1764 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003582.3 |
| RefSeq Size | 2207 bp |
| RefSeq ORF | 1767 bp |
| Locus ID | 8444 |
| UniProt ID | O43781 |
| Domains | pkinase |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 65.5 kDa |
| Gene Summary | This gene product belongs to the DYRK family of dual-specificity protein kinases that catalyze autophosphorylation on serine/threonine and tyrosine residues. The members of this family share structural similarity, however, differ in their substrate specificity, suggesting their involvement in different cellular functions. The encoded protein has been shown to autophosphorylate on tyrosine residue and catalyze phosphorylation of histones H3 and H2B in vitro. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224654L3 | Lenti-ORF clone of DYRK3 (Myc-DDK-tagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (DYRK3), transcript variant 1 |
CNY 6,460.00 |
|
| RC224654L4 | Lenti-ORF clone of DYRK3 (mGFP-tagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (DYRK3), transcript variant 1 |
CNY 6,460.00 |
|
| RG224654 | DYRK3 (tGFP-tagged) - Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (DYRK3), transcript variant 1 |
CNY 8,168.00 |
|
| SC117867 | DYRK3 (untagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (DYRK3), transcript variant 1 |
CNY 6,184.00 |
|
| SC318010 | DYRK3 (untagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (DYRK3), transcript variant 1 |
CNY 9,500.00 |
|
| SC323461 | DYRK3 (untagged)-Kinase deficient mutant (K238M) of Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (DYRK3), transcript variant 1 |
CNY 6,184.00 |
