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TRHDE (NM_013381) Human Tagged ORF Clone
CAT＃: RC224738
TRHDE (Myc-DDK-tagged)-Human thyrotropin-releasing hormone degrading enzyme (TRHDE)
ORF Plasmid: tGFP
"NM_013381" in other vectors (6)
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CNY 7,128.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PAP-II; PGPEP2; TRH-DE |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224738 representing NM_013381
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGAAGACGACGCCGCGCTTCGGGCTGGCAGCAGGGGGCTCTCCGACCCGTGGGCAGACTCAGTGG GAGTGCGACCCCGCACCACGGAGCGCCACATCGCCGTACACAAGCGGCTTGTGCTGGCCTTCGCTGTGTC CCTCGTGGCATTGCTCGCGGTCACAATGCTCGCTGTGCTGCTCAGCCTGCGCTTCGACGAGTGCGGGGCG AGTGCCACGCCAGGCGCCGACGGTGGCCCCTCAGGCTTTCCGGAGCGCGGCGGCAACGGGAGCCTCCCTG GATCGGCCCGGCGCAACCACCACGCAGGCGGGGACTCCTGGCAGCCCGAGGCGGGTGGGGTGGCCAGTCC GGGGACCACGTCGGCCCAGCCGCCGTCGGAGGAGGAGCGGGAGCCGTGGGAGCCGTGGACGCAGCTGCGC CTGTCGGGCCACCTGAAGCCGCTGCACTACAATCTGATGCTCACCGCCTTCATGGAGAACTTCACCTTCT CCGGGGAGGTCAACGTGGAGATCGCGTGCCGGAACGCCACCCGCTACGTAGTGCTGCACGCTTCCCGAGT GGCGGTGGAGAAAGTGCAGCTGGCCGAGGACCGGGCGTTCGGGGCTGTCCCTGTAGCCGGTTTTTTCCTC TACCCGCAAACCCAGGTCTTAGTGGTGGTGCTGAATAGGACACTGGACGCGCAGAGGAATTACAATCTGA AGATTATCTACAACGCGCTCATCGAGAATGAGCTCCTGGGCTTCTTCCGCAGCTCCTATGTGCTCCACGG GGAGAGAAGATTCCTTGGTGTTACTCAGTTTTCGCCTACACATGCCAGAAAGGCATTTCCTTGTTTTGAT GAGCCAATCTACAAGGCTACTTTCAAAATCAGCATCAAGCATCAAGCAACCTATTTATCTTTATCTAATA TGCCAGTGGAAACTTCCGTGTTTGAGGAAGATGGATGGGTTACGGATCACTTTTCACAGACCCCTCTCAT GTCCACATATTATTTAGCCTGGGCAATTTGCAACTTCACATACAGAGAAACTACCACCAAGAGTGGGGTT GTAGTACGATTATATGCAAGACCTGATGCTATCAGAAGAGGATCCGGGGACTATGCTCTCCATATAACAA AGAGATTAATAGAATTTTATGAAGACTACTTTAAAGTGCCCTATTCCTTGCCAAAACTAGATCTTTTAGC TGTGCCTAAGCATCCGTATGCTGCTATGGAGAACTGGGGACTAAGTATTTTTGTGGAACAAAGAATACTG CTGGATCCCAGTGTTTCATCTATTTCTTATTTGCTGGATGTCACCATGGTCATTGTTCATGAGATATGTC ACCAGTGGTTTGGTGACCTTGTGACGCCTGTGTGGTGGGAAGACGTGTGGCTGAAGGAAGGGTTTGCTCA CTACTTTGAATTTGTTGGTACAGACTACCTCTATCCTGGCTGGAACATGGAAAAGCAGAGGTTTCTGACC GATGTTCTGCATGAAGTGATGCTGCTGGACGGTTTGGCCAGTTCCCATCCAGTATCACAGGAAGTGCTGC AGGCAACAGATATTGACAGGGTGTTTGACTGGATCGCATATAAAAAGGGTGCTGCTTTAATAAGAATGCT GGCTAATTTTATGGGCCATTCAGTTTTCCAGAGGGGTTTGCAAGATTATTTAACCATTCATAAGTATGGT AATGCAGCCAGAAATGATCTCTGGAATACATTATCGGAGGCTTTAAAAAGAAATGGGAAATATGTAAATA TACAAGAAGTAATGGATCAGTGGACACTCCAGATGGGTTATCCTGTTATCACCATCTTGGGAAACACAAC AGCAGAAAATAGAATAATAATTACCCAACAGCATTTTATCTATGATATCAGTGCTAAAACTAAAGCACTT AAACTTCAGAATAACAGTTACCTGTGGCAGATTCCATTAACTATTGTGGTAGGAAATAGAAGCCATGTGT CTTCAGAAGCAATTATTTGGGTGTCTAACAAATCAGAGCACCACAGAATAACTTATTTGGACAAAGGAAG CTGGCTGCTGGGGAACATCAATCAAACTGGCTATTTTAGAGTCAACTATGACCTAAGGAACTGGAGATTA TTAATTGATCAATTAATCCGGAATCATGAGGTTCTTTCTGTCAGTAACCGAGCGGGCTTGATCGATGATG CCTTCAGCCTAGCCAGGGCTGGCTATTTGCCTCAGAATATTCCTCTGGAGATTATCAGATACCTGTCTGA GGAGAAGGATTTTCTTCCTTGGCATGCTGCCAGCCGAGCTCTTTATCCTCTAGATAAATTACTGGACCGC ATGGAAAACTACAACATTTTCAATGAATATATTTTAAAGCAAGTTGCAACAACATATATCAAGCTTGGGT GGCCGAAAAATAATTTTAATGGATCTCTTGTTCAAGCATCCTACCAACATGAAGAACTACGTAGAGAAGT TATAATGCTGGCCTGCAGTTTTGGCAACAAGCACTGTCACCAACAGGCATCAACACTTATTTCAGATTGG ATTTCCAGCAACAGGAACAGAATACCACTAAATGTTAGAGACATCGTATACTGTACAGGAGTGTCACTAC TGGATGAGGATGTCTGGGAATTCATATGGATGAAATTCCATTCCACCACAGCAGTTTCTGAGAAGAAAAT ATTATTGGAAGCCTTAACTTGCAGTGATGACAGGAATTTATTAAACAGGCTTCTAAATCTGTCACTGAAT TCTGAGGTGGTGCTGGATCAAGATGCAATTGATGTCATAATCCATGTAGCTCGAAATCCACATGGTCGAG ACCTTGCCTGGAAGTTTTTCAGGGATAAATGGAAGATATTAAATACCAGGTATGGAGAAGCATTGTTTAT GAATTCCAAACTCATCAGTGGTGTCACAGAATTTCTTAATACTGAAGGTGAACTCAAAGAGCTCAAGAAC TTCATGAAAAACTATGATGGGGTAGCTGCTGCTTCTTTCTCACGAGCTGTGGAAACTGTCGAAGCCAATG TGCGCTGGAAAATGCTTTACCAAGACGAGCTTTTCCAATGGTTAGGAAAAGCTCTAAGACAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224738 representing NM_013381
Red=Cloning site Green=Tags(s) MGEDDAALRAGSRGLSDPWADSVGVRPRTTERHIAVHKRLVLAFAVSLVALLAVTMLAVLLSLRFDECGA SATPGADGGPSGFPERGGNGSLPGSARRNHHAGGDSWQPEAGGVASPGTTSAQPPSEEEREPWEPWTQLR LSGHLKPLHYNLMLTAFMENFTFSGEVNVEIACRNATRYVVLHASRVAVEKVQLAEDRAFGAVPVAGFFL YPQTQVLVVVLNRTLDAQRNYNLKIIYNALIENELLGFFRSSYVLHGERRFLGVTQFSPTHARKAFPCFD EPIYKATFKISIKHQATYLSLSNMPVETSVFEEDGWVTDHFSQTPLMSTYYLAWAICNFTYRETTTKSGV VVRLYARPDAIRRGSGDYALHITKRLIEFYEDYFKVPYSLPKLDLLAVPKHPYAAMENWGLSIFVEQRIL LDPSVSSISYLLDVTMVIVHEICHQWFGDLVTPVWWEDVWLKEGFAHYFEFVGTDYLYPGWNMEKQRFLT DVLHEVMLLDGLASSHPVSQEVLQATDIDRVFDWIAYKKGAALIRMLANFMGHSVFQRGLQDYLTIHKYG NAARNDLWNTLSEALKRNGKYVNIQEVMDQWTLQMGYPVITILGNTTAENRIIITQQHFIYDISAKTKAL KLQNNSYLWQIPLTIVVGNRSHVSSEAIIWVSNKSEHHRITYLDKGSWLLGNINQTGYFRVNYDLRNWRL LIDQLIRNHEVLSVSNRAGLIDDAFSLARAGYLPQNIPLEIIRYLSEEKDFLPWHAASRALYPLDKLLDR MENYNIFNEYILKQVATTYIKLGWPKNNFNGSLVQASYQHEELRREVIMLACSFGNKHCHQQASTLISDW ISSNRNRIPLNVRDIVYCTGVSLLDEDVWEFIWMKFHSTTAVSEKKILLEALTCSDDRNLLNRLLNLSLN SEVVLDQDAIDVIIHVARNPHGRDLAWKFFRDKWKILNTRYGEALFMNSKLISGVTEFLNTEGELKELKN FMKNYDGVAAASFSRAVETVEANVRWKMLYQDELFQWLGKALRH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013381 |
| ORF Size | 3072 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013381.2 |
| RefSeq Size | 5666 bp |
| RefSeq ORF | 3210 bp |
| Locus ID | 29953 |
| UniProt ID | Q9UKU6 |
| Domains | Peptidase_M1 |
| Protein Families | Druggable Genome, Protease, Transmembrane |
| MW | 116.8 kDa |
| Gene Summary | This gene encodes a member of the peptidase M1 family. The encoded protein is an extracellular peptidase that specifically cleaves and inactivates the neuropeptide thyrotropin-releasing hormone.[provided by RefSeq, Dec 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224738L1 | Lenti ORF clone of Human thyrotropin-releasing hormone degrading enzyme (TRHDE), Myc-DDK-tagged |
CNY 9,528.00 |
|
| RC224738L2 | Lenti ORF clone of Human thyrotropin-releasing hormone degrading enzyme (TRHDE), mGFP tagged |
CNY 7,600.00 |
|
| RC224738L3 | Lenti ORF clone of Human thyrotropin-releasing hormone degrading enzyme (TRHDE), Myc-DDK-tagged |
CNY 7,600.00 |
|
| RC224738L4 | Lenti ORF clone of Human thyrotropin-releasing hormone degrading enzyme (TRHDE), mGFP tagged |
CNY 9,528.00 |
|
| RG224738 | TRHDE (tGFP-tagged) - Human thyrotropin-releasing hormone degrading enzyme (TRHDE) |
CNY 8,728.00 |
|
| SC115238 | TRHDE (untagged)-Human thyrotropin-releasing hormone degrading enzyme (TRHDE) |
CNY 7,136.00 |
