FAM105B (OTULIN) (NM_138348) Human Tagged ORF Clone
CAT#: RC224840
FAM105B (Myc-DDK-tagged)-Human family with sequence similarity 105, member B (FAM105B)
ORF Plasmid: tGFP
"NM_138348" in other vectors (7)
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CNY 5,488.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AIPDS; FAM105B; GUM |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC224840 representing NM_138348
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCGGGGGACTATGCCCCAGCCCGAAGCGTGGCCAGGCGCGAGCTGCGCCGAGACGCCGGCGCGGG AGGCGGCGGCCACGGCGCGGGACGGCGGGAAGGCGGCGGCCAGCGGGCAGCCGCGGCCCGAGATGCAGTG CCCGGCCGAGCATGAGGAGGACATGTACCGTGCTGCAGATGAAATAGAAAAGGAGAAAGAATTGCTTATA CATGAAAGAGGGGCATCAGAACCGAGATTAAGCGTAGCTCCTGAAATGGATATCATGGACTACTGCAAAA AAGAATGGAGAGGAAATACACAGAAAGCAACGTGTATGAAAATGGGCTATGAAGAGGTTTCTCAGAAGTT CACCTCCATACGGCGAGTCCGTGGTGATAATTACTGTGCACTGAGGGCCACGCTGTTCCAGGCCATGAGC CAGGCTGTGGGGCTGCCGCCCTGGCTGCAGGACCCGGAGCTCATGCTGTTACCAGAAAAACTCATAAGCA AATACAACTGGATCAAGCAATGGAAACTTGGACTGAAATTTGATGGGAAGAATGAGGACCTGGTTGATAA AATTAAAGAGTCCCTTACTCTGCTGAGGAAGAAGTGGGCAGGCTTGGCTGAAATGAGAACTGCTGAAGCA AGACAGATAGCTTGTGATGAACTATTCACAAATGAGGCGGAGGAATATAGCCTCTATGAAGCTGTAAAAT TTCTAATGCTAAACAGAGCCATTGAACTATATAATGATAAAGAGAAAGGAAAGGAAGTACCATTTTTCTC TGTGCTTCTGTTTGCTCGGGACACATCAAATGACCCAGGACAGCTTCTGAGGAACCACCTCAACCAGGTG GGACACACTGGTGGTCTTGAACAGGTTGAAATGTTCCTTCTTGCCTATGCTGTGCGCCACACCATCCAGG TGTACCGGCTCTCCAAGTACAACACGGAAGAATTCATCACAGTCTACCCCACCGACCCACCCAAGGACTG GCCAGTGGTAACGCTCATTGCTGAGGACGATCGGCACTATAACATCCCCGTCAGAGTGTGTGAGGAGACC AGTCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224840 representing NM_138348
Red=Cloning site Green=Tags(s) MSRGTMPQPEAWPGASCAETPAREAAATARDGGKAAASGQPRPEMQCPAEHEEDMYRAADEIEKEKELLI HERGASEPRLSVAPEMDIMDYCKKEWRGNTQKATCMKMGYEEVSQKFTSIRRVRGDNYCALRATLFQAMS QAVGLPPWLQDPELMLLPEKLISKYNWIKQWKLGLKFDGKNEDLVDKIKESLTLLRKKWAGLAEMRTAEA RQIACDELFTNEAEEYSLYEAVKFLMLNRAIELYNDKEKGKEVPFFSVLLFARDTSNDPGQLLRNHLNQV GHTGGLEQVEMFLLAYAVRHTIQVYRLSKYNTEEFITVYPTDPPKDWPVVTLIAEDDRHYNIPVRVCEET SL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_138348 |
ORF Size | 1056 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_138348.6 |
RefSeq Size | 7896 bp |
RefSeq ORF | 1059 bp |
Locus ID | 90268 |
UniProt ID | Q96BN8 |
MW | 40.1 kDa |
Gene Summary | This gene encodes a member of the peptidase C65 family of ubiquitin isopeptidases. Members of this family remove ubiquitin from proteins. The encoded enzyme specifically recognizes and removes M1(Met1)-linked, or linear, ubiquitin chains from protein substrates. Linear ubiquitin chains are known to regulate the NF-kappa B signaling pathway in the context of immunity and inflammation. Mutations in this gene cause a potentially fatal autoinflammatory syndrome in human patients. [provided by RefSeq, Sep 2016] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
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Linear ubiquitination by LUBEL has a role in Drosophila heat stress response
,Asaoka, T;Almagro, J;Ehrhardt, C;Tsai, I;Schleiffer, A;Deszcz, L;Junttila, S;Ringrose, L;Mechtler, K;Kavirayani, A;Gyenesei, A;Hofmann, K;Duchek, P;Rittinger, K;Ikeda, F;,
EMBO Rep.
,PubMed ID 27702987
[FAM105B]
|
Biallelic hypomorphic mutations in a linear deubiquitinase define otulipenia, an early-onset autoinflammatory disease
,Zhou, Q;Yu, X;Demirkaya, E;Deuitch, N;Stone, D;Tsai, WL;Kuehn, HS;Wang, H;Yang, D;Park, YH;Ombrello, AK;Blake, M;Romeo, T;Remmers, EF;Chae, JJ;Mullikin, JC;Güzel, F;Milner, JD;Boehm, M;Rosenzweig, SD;Gadina, M;Welch, SB;Özen, S;Topaloglu, R;Abinun, M;Kastner, DL;Aksentijevich, I;,
Proc. Natl. Acad. Sci. U.S.A.
,PubMed ID 27559085
[FAM105B]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC224840L1 | Lenti ORF clone of Human family with sequence similarity 105, member B (FAM105B), Myc-DDK-tagged |
CNY 7,888.00 |
|
RC224840L2 | Lenti ORF clone of Human family with sequence similarity 105, member B (FAM105B), mGFP tagged |
CNY 5,890.00 |
|
RC224840L3 | Lenti ORF clone of Human family with sequence similarity 105, member B (FAM105B), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC224840L4 | Lenti ORF clone of Human family with sequence similarity 105, member B (FAM105B), mGFP tagged |
CNY 7,888.00 |
|
RG224840 | FAM105B (tGFP-tagged) - Human family with sequence similarity 105, member B (FAM105B) |
CNY 7,088.00 |
|
SC100495 | FAM105B (untagged)-Human family with sequence similarity 105, member B (FAM105B) |
CNY 26,064.00 |
|
SC317556 | FAM105B (untagged)-Human family with sequence similarity 105, member B (FAM105B) |
CNY 5,488.00 |
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