ZNF160 (NM_001102603) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	F11; HKr18; HZF5; KR18
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC224891 representing NM_001102603. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCCTTACTCAGGTACGGTTGACATTTAGGGATGTGGCCATAGAATTCTCTCAGGAGGAGTGGAAA TGCCTGGACCCTGCTCAGAGGATCTTATACAGGGACGTGATGTTGGAGAACTACTGGAACCTTGTTTCT CTGGGACTGTGTCATTTTGATATGAATATTATCTCCATGTTGGAGGAAGGGAAAGAGCCCTGGACTGTG AAGAGCTGTGTGAAAATAGCAAGAAAACCAAGAACGCGGGAATGTGTCAAAGGCGTGGTCACAGATATC CCTCCTAAATGTACAATCAAGGATTTGCTACCAAAAGAGAAGAGCAGTACAGAAGCAGTATTCCACACA GTGGTGTTGGAAAGACACGAAAGCCCTGACATTGAAGACTTTTCCTTCAAGGAACCCCAGAAAAATGTG CATGATTTTGAGTGTCAATGGAGAGATGACACAGGAAATTACAAGGGAGTGCTTATGGCCCAGAAAGAA GGTAAAAGAGATCAACGCGACAGAAGAGACATAGAAAACAAGCTTATGAACAATCAGCTTGGAGTAAGC TTTCATTCTCATCTGCCTGAACTGCAGCTATTTCAAGGTGAGGGGAACATGTATGAATGTAATCAAGTT GAGAAGTCTACCAACAATGGTTCCTCAGTGTCACCACTTCAACAAATTCCTTCTAGTGTCCAAACCCAC AGGTCTAAAAAATATCATGAACTTAACCATTTTTCATTACTCACACAAAGACGAAAAGCAAACAGTTGT GGAAAACCTTATAAATGTAATGAATGTGGCAAGGCGTTCACTCAGAATTCGAACCTTACAAGTCATAGG AGAATTCATAGTGGAGAGAAGCCTTACAAATGCAGTGAGTGCGGCAAAACCTTTACTGTTCGTTCAAAT CTAACTATTCATCAGGTCATCCATACTGGAGAAAAACCTTACAAATGTCATGAGTGTGGCAAGGTCTTC AGGCACAATTCATACCTTGCAACTCATCGGCGAATTCATACTGGAGAGAAACCTTACAAGTGTAATGAG TGTGGAAAAGCCTTTAGAGGACATTCAAACCTAACTACCCATCAGTTAATTCATACTGGAGAAAAACCG TTCAAATGTAATGAATGTGGCAAGCTCTTCACTCAAAATTCACACCTTATAAGTCATTGGAGAATTCAC ACTGGAGAGAAACCTTACAAGTGCAATGAGTGCGGCAAAGCCTTTAGTGTTCGTTCAAGCCTAGCAATC CATCAGACAATCCACACTGGAGAAAAACCTTACAAATGTAATGAATGTGGCAAAGTCTTTAGGTACAAT TCATACCTCGGAAGGCATCGGAGAGTTCATACTGGTGAGAAACCTTACAAGTGTAATGAATGTGGCAAA GCCTTCAGTATGCATTCAAACCTAGCTACCCATCAGGTCATCCATACTGGAACAAAACCTTTCAAATGC AATGAATGCAGCAAGGTTTTCACTCAAAATTCACAACTTGCAAATCATCGAAGAATTCATACTGGAGAG AAACCTTACAAGTGTAATGAGTGTGGGAAAGCCTTCAGTGTTCGTTCAAGTCTGACTACCCATCAGGCA ATCCATTCTGGAGAGAAACCTTACAAATGTATTGAATGTGGCAAGAGCTTCACTCAAAAATCACACCTT AGAAGTCATCGGGGAATTCATTCTGGAGAGAAACCTTACAAGTGTAATGAATGTGGTAAAGTCTTCGCT CAAACATCACAACTTGCAAGGCATTGGAGAGTTCATACTGGAGAAAAACCTTACAAGTGTAATGACTGT GGCAGAGCCTTTAGTGATCGTTCAAGCCTAACTTTTCATCAGGCAATACATACTGGAGAGAAACCTTAC AAATGTCATGAATGCGGCAAGGTTTTTAGGCACAATTCATACCTTGCAACTCATCGGCGAATTCATACT GGAGAGAAACCTTACAAGTGTAATGAGTGTGGGAAAGCCTTTAGTATGCATTCAAACCTAACTACCCAT AAGGTCATCCATACTGGAGAGAAGCCTTACAAATGTAATCAATGTGGCAAGGTCTTCACTCAGAACTCA CACCTTGCAAATCATCAAAGGACTCACACCGGAGAGAAACCTTACCGATGCAATGAGTGTGGGAAAGCC TTCAGTGTTCGTTCAAGCCTAACCACCCATCAGGCAATCCATACTGGGAAAAAACCTTACAAATGTAAT GAATGTGGCAAGGTCTTTACTCAAAATGCTCACCTGGCAAATCACCGAAGAATTCATACTGGGGAGAAA CCTTACAGGTGTACAGAGTGTGGGAAAGCCTTTAGGGTAAGATCAAGTCTAACTACCCATATGGCAATC CACACTGGAGAAAAGCGTTACAAATGTAATGAGTGTGGCAAGGTCTTCAGGCAGAGTTCAAATCTTGCA AGTCATCACAGAATGCATACCGGAGAGAAACCTTACAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC Protein Sequence (showhide) >Peptide sequence encoded by RC224891 Blue=ORF Red=Cloning site Green=Tag(s) MALTQVRLTFRDVAIEFSQEEWKCLDPAQRILYRDVMLENYWNLVSLGLCHFDMNIISMLEEGKEPWTV KSCVKIARKPRTRECVKGVVTDIPPKCTIKDLLPKEKSSTEAVFHTVVLERHESPDIEDFSFKEPQKNV HDFECQWRDDTGNYKGVLMAQKEGKRDQRDRRDIENKLMNNQLGVSFHSHLPELQLFQGEGNMYECNQV EKSTNNGSSVSPLQQIPSSVQTHRSKKYHELNHFSLLTQRRKANSCGKPYKCNECGKAFTQNSNLTSHR RIHSGEKPYKCSECGKTFTVRSNLTIHQVIHTGEKPYKCHECGKVFRHNSYLATHRRIHTGEKPYKCNE CGKAFRGHSNLTTHQLIHTGEKPFKCNECGKLFTQNSHLISHWRIHTGEKPYKCNECGKAFSVRSSLAI HQTIHTGEKPYKCNECGKVFRYNSYLGRHRRVHTGEKPYKCNECGKAFSMHSNLATHQVIHTGTKPFKC NECSKVFTQNSQLANHRRIHTGEKPYKCNECGKAFSVRSSLTTHQAIHSGEKPYKCIECGKSFTQKSHL RSHRGIHSGEKPYKCNECGKVFAQTSQLARHWRVHTGEKPYKCNDCGRAFSDRSSLTFHQAIHTGEKPY KCHECGKVFRHNSYLATHRRIHTGEKPYKCNECGKAFSMHSNLTTHKVIHTGEKPYKCNQCGKVFTQNS HLANHQRTHTGEKPYRCNECGKAFSVRSSLTTHQAIHTGKKPYKCNECGKVFTQNAHLANHRRIHTGEK PYRCTECGKAFRVRSSLTTHMAIHTGEKRYKCNECGKVFRQSSNLASHHRMHTGEKPYK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001102603
ORF Size	2454 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq Size	4336 bp
RefSeq ORF	2457 bp
Locus ID	90338
UniProt ID	Q9HCG1
Protein Families	Transcription Factors
MW	94.2 kDa
Gene Summary	The protein encoded by this gene is a Kruppel-related zinc finger protein which is characterized by the presence of an N-terminal repressor domain, the Kruppel-associated box (KRAB). The KRAB domain is a potent repressor of transcription; thus this protein may function in transcription regulation. Multiple transcript variants have been found for this gene. [provided by RefSeq, Apr 2016]

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