ZNF658B (NM_001032297) Human Tagged ORF Clone
CAT#: RC224897
- TrueORF®
ZNF658B (Myc-DDK-tagged)-Human zinc finger protein 658B (ZNF658B). Note: ORF is codon optimized
ORF Plasmid: tGFP
"NM_001032297" in other vectors (3)
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CNY 6,000.00
CNY 8,170.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | zinc finger protein 658B |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC224897 ORF sequence, codon optimized.
Due to the complexity of NM_001032297, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAACCTCGAAAAAAATTTCGATAAAACCACTTTGTTTAATCATATGAGGACGGATAAACGCGGCA AGTGCTCTGATCTCAATGAATATGGCACGTCCTGTGATAAGACTACCGCAGTTGAGTACAATAAGGTGCA TATGGCTATGACTCACTATGAATGCAATGAACGAGGGATCAACTTCAGCAGGAAGTCCCCTCTGACCCAG TCACAACGCACGATTACAGGATGGAGCGCGTTCGAGTCCAATAAATGTGAGGAGAATTTTTCCCAGTCAT CCGCCCATATCGTGCACCAGAAGACACAGGCTGGAGATAAGTTCGGCGAACATAATGAGTGTACCGATGC ACTTTATCAGAAATTGGACTTTACCGCCCACCAGCGGATCCATACAGACTTCACCGCTCACCAAAAATTC TACCTGTCAGACGAACACGGAAAGTGTAGAAAATCCTTTTACTGGAAGGCGCACCTTATTCAGCATGAGA GGCCTCATAGCGGCGAGAAGACTTATCAATACGAGGAATGCGCTAAGTCCTTCTGTAGTTCCTCCCATCC CATCCAGCACCCTGGGACGTACGTGGGCTTTAAGTTGTATGAGTGCAACGAGTGCGGTAAGGCGTTCTGC CAGAACTCCAACCTCTCCAAACATCTCAGAATACACACTAAGGAAAAACCATGCGATAATAATGGCTGCG GGAGAAGCTATAAATCTCCTCTGATCGGCCACCAGAAGACTGACGCTGAAATGGAACTGTGCGGAGGCTC TGAGTATGGCAAAACTAGCCACCTGAAGGGACATCAAAGGATTCTGATGGGGGAAAAACCTTATGAGTGC ATCGAGTGTGGCAAGACCTTCTCTAAGACCAGCCATCTCCGGGCGCACCAAAGGATTCATACTGGGGAAA AACCCTACGAATGTGTGGAGTGTGAGAAGACATTCTCTCACAAGACGCATTTGTCAGTTCACCAGCGCGT ACACACAGGAGAAAAGCCATACGAGTGCAACGATTGTGGCAAGAGCTTCACATATAATAGCGCTTTGAGG GCACACCAGCGCATTCACACGGGTGAGAAGCCATATGAATGCAGCGACTGTGAAAAGACCTTCGCTCATA ATTCAGCGCTGAGAGCTCATCACCGGATCCATACAGGCGAAAAGCCATACGAGTGCAATGAATGCGGAAG GTCATTTGCCCATATTTCCGTGCTTAAGGCCCACCAGCGCATCCACACCCGAGAGAAGCCATATGAATGC AATGAGTGTGGGAGAAGTTTCACCTATAACTCAGCACTGCGGGCCCACCAACGGATTCACACAGGCCGGA AGCCCTACGAGTGCAGTGATTGCGAGAAAACCTTCGCCCATAATAGCGCTCTGAAAATACATCAGAGGAT CCATACTGGCGAGAAGCCCTACAAGTGCAATGAGTGCGAAAAAACATTCGCTCACAACTCAGCCCTGAGA GCCCACCAAAACATCCATACGGGTGAAAAGCTTTATGAGTGCAGTGAGTGCGGCAAAACTTTTTTTCAAA AGACTAGGCTCTCAACTCACCGGAGGATTCACACAGGGGAGAAACCTTATGAGTGTAGCAAGTGCGGGAA GACATTTTCACAAAAAAGTTACCTCAGCGGCCACGAGCGGATCCACACGGGAGAAAAGCCATATGAGTGT AATGTTTGCGGGAAAACCTTCGTGTACAAAGCCGCCCTGATTGTTCATCAGAGGATTCATACCGGCGAGA AGCCCTACGAGTGTAATGAATGTGGCAAAACTTTTTCCCAGAGAACACACCTCTGCGCTCATCAAAGGAT TCACACAGGAGAAAAGCCCTACGAGTGTAATGAGTGCGGCAAGACCTTTGCCGATAATTCCGCCCTCAGG GCCCACCATCGCATCCACACTGGAGAGAAACCTTACGAATGCAACGACTGTGGCAAGACCTTCTCCAAGA CTAGTCATCTGAGAGCGCACCTTCGGACCAGGTCTGGGGAAAAGCCCTATGAATGCAGCGAGTGCGGAAA AACTTTCTCAGAGAAATCATACGTGTCAGCTCATCAGAGAGTTCACACAGGCGAAAAACCATATGAGTGT AATGTGTGCGGAAAACCATTCGCACATAACTCTACTCTGAGGGTTCACCAGCGGATTCACACAGGAGAGA AGTCCTACGAATGCAACGATTGCGGCAAGACCTTCTCTCAAAAGAGCCACTTGTCAGCACATCAGCGAAT CCATACCGGCGAAAAGCCTTACGAGTGCAACGAGTGCGGAAAGGCTTTCGCCCAGAATAGTACCCTCAGG GTCCATCAGAGAATCCACACAGGAGAAAAACCATACGAATGCGATGAGTGCGGGAAGACGTTCGTTCGAA AAGCCGCCCTTCGGGTGCATCACACCAGAATGCACACACGGGAGAAAACGCTGGCTTGTAATGGGTTTGG CAAATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224897 representing NM_001032297
Red=Cloning site Green=Tags(s) MMNLEKNFDKTTLFNHMRTDKRGKCSDLNEYGTSCDKTTAVEYNKVHMAMTHYECNERGINFSRKSPLTQ SQRTITGWSAFESNKCEENFSQSSAHIVHQKTQAGDKFGEHNECTDALYQKLDFTAHQRIHTDFTAHQKF YLSDEHGKCRKSFYWKAHLIQHERPHSGEKTYQYEECAKSFCSSSHPIQHPGTYVGFKLYECNECGKAFC QNSNLSKHLRIHTKEKPCDNNGCGRSYKSPLIGHQKTDAEMELCGGSEYGKTSHLKGHQRILMGEKPYEC IECGKTFSKTSHLRAHQRIHTGEKPYECVECEKTFSHKTHLSVHQRVHTGEKPYECNDCGKSFTYNSALR AHQRIHTGEKPYECSDCEKTFAHNSALRAHHRIHTGEKPYECNECGRSFAHISVLKAHQRIHTREKPYEC NECGRSFTYNSALRAHQRIHTGRKPYECSDCEKTFAHNSALKIHQRIHTGEKPYKCNECEKTFAHNSALR AHQNIHTGEKLYECSECGKTFFQKTRLSTHRRIHTGEKPYECSKCGKTFSQKSYLSGHERIHTGEKPYEC NVCGKTFVYKAALIVHQRIHTGEKPYECNECGKTFSQRTHLCAHQRIHTGEKPYECNECGKTFADNSALR AHHRIHTGEKPYECNDCGKTFSKTSHLRAHLRTRSGEKPYECSECGKTFSEKSYVSAHQRVHTGEKPYEC NVCGKPFAHNSTLRVHQRIHTGEKSYECNDCGKTFSQKSHLSAHQRIHTGEKPYECNECGKAFAQNSTLR VHQRIHTGEKPYECDECGKTFVRKAALRVHHTRMHTREKTLACNGFGKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001032297 |
ORF Size | 2457 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001032297.1, NP_001027468.1 |
RefSeq Size | 3375 bp |
RefSeq ORF | 2459 bp |
Locus ID | 401509 |
MW | 94.3 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC224897L3 | Lenti-ORF clone of ZNF658B (Myc-DDK-tagged)-Human zinc finger protein 658B (ZNF658B). Note: ORF is codon optimized |
CNY 10,070.00 |
|
RC224897L4 | Lenti-ORF clone of ZNF658B (mGFP-tagged)-Human zinc finger protein 658B (ZNF658B). Note: ORF is codon optimized |
CNY 10,070.00 |
|
RG224897 | ZNF658B (tGFP-tagged) - Human zinc finger protein 658B (ZNF658B). Note: ORF is codon optimized |
CNY 9,030.00 |
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