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Paxillin (PXN) (NM_025157) Human Tagged ORF Clone
CAT＃: RC225692
PXN (Myc-DDK-tagged)-Human paxillin (PXN), transcript variant 3
ORF Plasmid: tGFP
"NM_025157" in other vectors (5)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC225692 representing NM_025157
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCACGTCCCTGGGCAGCAACCTTTCTGAACTCGACCGCCTGCTGCTGGAACTGAACGCTGTACAGC ATAACCCGCCAGGCTTCCCTGCAGATGAGGCCAACTCAAGCCCCCCGCTTCCTGGGGCCCTGAGCCCCCT CTATGGTGTCCCAGAGACTAACAGCCCCTTGGGAGGCAAAGCTGGGCCCCTGACGAAAGAGAAGCCTAAG CGGAATGGGGGCCGGGGCCTGGAGGACGTGCGGCCCAGTGTGGAGAGTCTCTTGGATGAACTGGAGAGCT CCGTGCCCAGCCCCGTCCCTGCCATCACTGTGAACCAGGGCGAGATGAGCAGCCCGCAGCGCGTCACCTC CACCCAACAGCAGACACGCATCTCGGCCTCCTCTGCCACCAGGGAGCTGGACGAGCTGATGGCTTCGCTG TCGGATTTCAAGTTCATGGCCCAGGGGAAGACAGGGAGCAGCTCACCCCCTGGGGGGCCCCCGAAGCCCG GGAGCCAGCTGGACAGCATGCTGGGGAGCCTGCAGTCTGACCTGAACAAGCTGGGGGTCGCCACAGTCGC CAAAGGAGTCTGCGGGGCCTGCAAGAAGCCCATCGCCGGGCAGGTTGTGACCGCCATGGGGAAGACGTGG CACCCCGAGCACTTCGTCTGCACCCACTGCCAGGAGGAGATCGGATCCCGGAACTTCTTCGAGCGGGATG GACAGCCCTACTGTGAAAAGGACTACCACAACCTCTTCTCCCCGCGCTGCTACTACTGCAACGGCCCCAT CCTGGATAAAGTGGTGACAGCCCTTGACCGGACGTGGCACCCTGAACACTTCTTCTGTGCACAGTGTGGA GCCTTCTTTGGTCCCGAAGGGTTCCACGAGAAGGACGGCAAGGCCTACTGTCGCAAGGACTACTTCGACA TGTTCGCACCCAAGTGTGGCGGCTGCGCCCGGGCCATCCTGGAGAACTATATCTCAGCCCTCAACACGCT GTGGCATCCTGAGTGCTTTGTGTGCCGGGAATGCTTCACGCCATTCGTGAACGGCAGCTTCTTCGAGCAC GACGGGCAGCCCTACTGTGAGGTGCACTACCACGAGCGGCGCGGCTCGCTGTGTTCTGGCTGCCAGAAGC CCATCACCGGCCGCTGCATCACCGCCATGGCCAAGAAGTTCCACCCCGAGCACTTCGTCTGTGCCTTCTG CCTCAAGCAGCTCAACAAGGGCACCTTCAAGGAGCAGAACGACAAGCCTTACTGTCAGAACTGCTTCCTC AAGCTCTTCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC225692 representing NM_025157
Red=Cloning site Green=Tags(s) MSTSLGSNLSELDRLLLELNAVQHNPPGFPADEANSSPPLPGALSPLYGVPETNSPLGGKAGPLTKEKPK RNGGRGLEDVRPSVESLLDELESSVPSPVPAITVNQGEMSSPQRVTSTQQQTRISASSATRELDELMASL SDFKFMAQGKTGSSSPPGGPPKPGSQLDSMLGSLQSDLNKLGVATVAKGVCGACKKPIAGQVVTAMGKTW HPEHFVCTHCQEEIGSRNFFERDGQPYCEKDYHNLFSPRCYYCNGPILDKVVTALDRTWHPEHFFCAQCG AFFGPEGFHEKDGKAYCRKDYFDMFAPKCGGCARAILENYISALNTLWHPECFVCRECFTPFVNGSFFEH DGQPYCEVHYHERRGSLCSGCQKPITGRCITAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQNCFL KLFC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025157 |
| ORF Size | 1272 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_025157.5 |
| RefSeq ORF | 1275 bp |
| Locus ID | 5829 |
| UniProt ID | P49023 |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, VEGF signaling pathway |
| MW | 46.4 kDa |
| Gene Summary | This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC225692L3 | Lenti-ORF clone of PXN (Myc-DDK-tagged)-Human paxillin (PXN), transcript variant 3 |
CNY 5,890.00 |
|
| RC225692L4 | Lenti-ORF clone of PXN (mGFP-tagged)-Human paxillin (PXN), transcript variant 3 |
CNY 5,890.00 |
|
| RG225692 | PXN (tGFP-tagged) - Human paxillin (PXN), transcript variant 3 |
CNY 4,370.00 |
|
| SC312694 | PXN (untagged)-Human paxillin (PXN), transcript variant 3 |
CNY 7,220.00 |
|
| SC327847 | PXN (untagged)-Human paxillin (PXN) transcript variant 3 |
CNY 7,220.00 |
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