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RBM20 (NM_001134363) Human Tagged ORF Clone
CAT＃: RC226364
RBM20 (Myc-DDK-tagged)-Human RNA binding motif protein 20 (RBM20)
ORF Plasmid: tGFP
"NM_001134363" in other vectors (6)
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CNY 12,816.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226364 representing NM_001134363
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCTGGCAGCAGCCATGAGCCAGGACGCGGACCCCAGCGGTCCGGAGCAGCCGGACAGAGTTGCCT GCAGTGTGCCTGGTGCCCGGGCGTCCCCGGCACCCTCCGGCCCGCGAGGGATGCAGCAGCCGCCGCCGCC GCCCCAGCCACCGCCCCCGCCCCAAGCCGGCCTACCCCAGATCATCCAAAATGCCGCCAAGCTCCTGGAC AAGAACCCATTCTCGGTCAGTAACCCGAACCCTCTGCTTCCTTCACCTGCCAGTCTCCAGCTGGCTCAAC TGCAGGCCCAGCTCACCCTCCACCGGCTGAAGCTGGCACAGACAGCTGTCACCAACAACACTGCAGCCGC CACAGTCCTGAACCAAGTCCTCTCCAAAGTGGCCATGTCCCAGCCTCTCTTCAATCAACTGAGGCATCCG TCTGTGATCACTGGCCCCCACGGCCATGCTGGGGTTCCCCAACATGCTGCAGCCATACCCAGTACCCGGT TTCCCTCTAATGCAATTGCCTTTTCACCCCCCAGCCAGACACGAGGCCCCGGACCCTCCATGAACCTTCC CAACCAGCCACCCAGTGCCATGGTGATGCATCCTTTCACTGGGGTAATGCCTCAGACCCCTGGCCAGCCA GCAGTCATCTTGGGCATTGGCAAGACTGGGCCTGCTCCAGCTACAGCAGGATTCTATGAGTATGGCAAAG CCAGCTCTGGCCAGACATATGGCCCTGAAACAGATGGTCAGCCTGGCTTCCTGCCATCCTCGGCCTCAAC CTCGGGCAGTGTGACCTATGAAGGGCACTACAGCCACACAGGGCAGGATGGTCAAGCTGCCTTTTCCAAA GATTTTTACGGACCCAACTCCCAAGGTTCACATGTGGCCAGCGGATTTCCAGCTGAGCAGGCTGGGGGCC TGAAAAGTGAGGTCGGGCCACTGCTGCAGGGCACAAACAGCCAATGGGAGAGCCCCCATGGATTCTCGGG CCAAAGCAAGCCTGATCTCACAGCAGGTCCCATGTGGCCTCCACCCCACAACCAGCCCTATGAGCTGTAC GACCCCGAGGAACCAACCTCAGACAGGACACCTCCTTCCTTCGGGGGTCGGCTTAACAACAGCAAACAGG GTTTTATCGGTGCTGGGCGGAGGGCCAAGGAGGACCAGGCGTTGCTATCTGTGCGGCCCCTGCAGGCTCA TGAGCTGAACGACTTTCACGGTGTGGCCCCCCTCCACTTGCCGCATATCTGTAGCATCTGTGACAAGAAG GTGTTTGATTTGAAGGACTGGGAGCTGCATGTGAAAGGGAAGCTGCACGCTCAGAAATGCCTGGTCTTCT CTGAAAATGCTGGCATCCGGTGTATACTTGGTTCGGCAGAGGGAACATTGTGTGCTTCTCCCAACAGCAC AGCTGTTTATAACCCTGCTGGGAATGAAGATTATGCCTCAAATCTTGGAACATCATACGTGCCCATTCCA GCAAGGTCATTCACTCAGTCAAGCCCCACATTTCCTTTGGCTTCTGTGGGGACAACTTTTGCACAGCGGA AAGGGGCTGGCCGTGTGGTGCACATCTGCAATCTCCCTGAAGGAAGCTGCACTGAGAATGACGTCATTAA CCTGGGGCTGCCCTTTGGAAAGGTCACTAATTACATCCTCATGAAGTCGACTAATCAGGCCTTTTTAGAG ATGGCTTACACAGAAGCTGCACAGGCCATGGTCCAGTATTATCAAGAAAAATCTGCTGTGATCAATGGTG AGAAGTTGCTCATTCGGATGTCCAAGAGATACAAGGAATTGCAGCTCAAGAAACCCGGGAAGGCCGTGGC TGCCATCATCCAGGACATCCATTCCCAGAGGGAGAGGGACATGTTCCGGGAAGCAGACAGATATGGCCCA GAAAGGCCGCGGTCTCGTAGTCCGGTGAGCCGGTCACTCTCCCCGAGGTCCCACACTCCCAGCTTCACCT CCTGCAGCTCTTCCCACAGCCCTCCGGGCCCCTCCCGGGCTGACTGGGGCAATGGCCGGGACTCCTGGGA GCACTCTCCCTATGCCAGGAGGGAGGAAGAGCGAGACCCGGCTCCCTGGAGGGACAACGGAGATGACAAG AGGGACAGGATGGACCCCTGGGCACATGATCGCAAACACCACCCCCGGCAACTGGACAAGGCTGAGTTGG ACGAGCGACCAGAAGGAGGGAGGCCCCACCGGGAGAAGTACCCGAGATCTGGGTCTCCCAACCTGCCCCA CTCTGTGTCCAGCTACAAAAGCCGTGAAGACGGCTACTACCGGAAAGAGCCCAAAGCCAAGTGGGACAAG TATCTGAAGCAGCAGCAGGATGCCCCCGGGAGGTCCAGGAGGAAAGACGAGGCCAGGCTGCGGGAAAGCA GACACCCCCATCCGGATGACTCAGGCAAGGAAGATGGGCTGGGGCCAAAGGTCACTAGGGCCCCTGAGGG CGCCAAGGCCAAGCAGAATGAGAAAAATAAAACCAAGAGAACTGATAGAGACCAAGAAGGAGCTGATGAT AGAAAAGAAAACACAATGGCAGAGAATGAGGCTGGAAAAGAGGAACAGGAGGGCATGGAAGAAAGCCCTC AATCAGTGGGCAGACAGGAGAAAGAAGCAGAGTTCTCTGATCCGGAAAACACAAGGACAAAGAAGGAACA AGATTGGGAGAGTGAAAGTGAGGCAGAGGGGGAGAGCTGGTATCCCACTAACATGGAGGAGCTGGTGACA GTGGACGAGGTTGGGGAAGAAGAAGATTTTATCGTGGAACCAGACATCCCAGAGCTGGAAGAAATTGTGC CCATTGACCAGAAAGACAAAATTTGCCCAGAAACATGTCTGTGTGTGACAACCACCTTAGACTTAGACCT GGCCCAGGATTTCCCCAAGGAAGGAGTCAAGGCCGTAGGGAATGGGGCTGCAGAAATCAGCCTCAAGTCA CCCAGAGAACTGCCCTCTGCTTCCACAAGCTGTCCCAGTGACATGGACGTGGAAATGCCTGGCCTAAATC TGGATGCTGAGCGGAAGCCAGCTGAAAGTGAGACAGGCCTCTCCCTGGAGGATTCAGATTGCTACGAGAA GGAGGCAAAGGGAGTGGAGAGCTCAGATGTTCATCCAGCCCCTACAGTCCAGCAAATGTCTTCCCCTAAG CCAGCAGAGGAGAGGGCCCGGCAGCCAAGCCCATTTGTGGATGATTGCAAGACCAGGGGGACCCCCGAAG ATGGGGCTTGTGAAGGCAGCCCCCTGGAGGAGAAAGCCAGCCCCCCCATCGAAACTGACCTCCAAAACCA AGCTTGCCAAGAAGTGTTGACCCCGGAAAACTCCAGGTACGTGGAAATGAAATCTCTGGAGGTGAGGTCA CCAGAGTACACTGAAGTGGAACTGAAACAGCCCCTTTCTTTGCCCTCTTGGGAACCAGAGGATGTGTTCA GTGAACTTAGCATTCCTCTAGGGGTGGAGTTCGTGGTTCCCAGGACTGGCTTTTATTGCAAGCTGTGTGG GCTGTTCTACACGAGCGAGGAGACAGCAAAGATGAGCCACTGCCGCAGCGCTGTCCACTACAGGAACTTA CAGAAATATTTGTCCCAGCTGGCCGAGGAGGGCCTCAAGGAGACCGAGGGGGCAGATAGCCCGAGGCCAG AGGACAGCGGAATCGTGCCACGCTTCGAAAGGAAAAAGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226364 representing NM_001134363
Red=Cloning site Green=Tags(s) MVLAAAMSQDADPSGPEQPDRVACSVPGARASPAPSGPRGMQQPPPPPQPPPPPQAGLPQIIQNAAKLLD KNPFSVSNPNPLLPSPASLQLAQLQAQLTLHRLKLAQTAVTNNTAAATVLNQVLSKVAMSQPLFNQLRHP SVITGPHGHAGVPQHAAAIPSTRFPSNAIAFSPPSQTRGPGPSMNLPNQPPSAMVMHPFTGVMPQTPGQP AVILGIGKTGPAPATAGFYEYGKASSGQTYGPETDGQPGFLPSSASTSGSVTYEGHYSHTGQDGQAAFSK DFYGPNSQGSHVASGFPAEQAGGLKSEVGPLLQGTNSQWESPHGFSGQSKPDLTAGPMWPPPHNQPYELY DPEEPTSDRTPPSFGGRLNNSKQGFIGAGRRAKEDQALLSVRPLQAHELNDFHGVAPLHLPHICSICDKK VFDLKDWELHVKGKLHAQKCLVFSENAGIRCILGSAEGTLCASPNSTAVYNPAGNEDYASNLGTSYVPIP ARSFTQSSPTFPLASVGTTFAQRKGAGRVVHICNLPEGSCTENDVINLGLPFGKVTNYILMKSTNQAFLE MAYTEAAQAMVQYYQEKSAVINGEKLLIRMSKRYKELQLKKPGKAVAAIIQDIHSQRERDMFREADRYGP ERPRSRSPVSRSLSPRSHTPSFTSCSSSHSPPGPSRADWGNGRDSWEHSPYARREEERDPAPWRDNGDDK RDRMDPWAHDRKHHPRQLDKAELDERPEGGRPHREKYPRSGSPNLPHSVSSYKSREDGYYRKEPKAKWDK YLKQQQDAPGRSRRKDEARLRESRHPHPDDSGKEDGLGPKVTRAPEGAKAKQNEKNKTKRTDRDQEGADD RKENTMAENEAGKEEQEGMEESPQSVGRQEKEAEFSDPENTRTKKEQDWESESEAEGESWYPTNMEELVT VDEVGEEEDFIVEPDIPELEEIVPIDQKDKICPETCLCVTTTLDLDLAQDFPKEGVKAVGNGAAEISLKS PRELPSASTSCPSDMDVEMPGLNLDAERKPAESETGLSLEDSDCYEKEAKGVESSDVHPAPTVQQMSSPK PAEERARQPSPFVDDCKTRGTPEDGACEGSPLEEKASPPIETDLQNQACQEVLTPENSRYVEMKSLEVRS PEYTEVELKQPLSLPSWEPEDVFSELSIPLGVEFVVPRTGFYCKLCGLFYTSEETAKMSHCRSAVHYRNL QKYLSQLAEEGLKETEGADSPRPEDSGIVPRFERKKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001134363 |
| ORF Size | 3681 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001134363.1, NP_001127835.1 |
| RefSeq ORF | 3684 bp |
| Locus ID | 282996 |
| UniProt ID | Q5T481 |
| MW | 134.2 kDa |
| Gene Summary | This gene encodes a protein that binds RNA and regulates splicing. Mutations in this gene have been associated with familial dilated cardiomyopathy. [provided by RefSeq, Apr 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226364L1 | Lenti ORF clone of Human RNA binding motif protein 20 (RBM20), Myc-DDK-tagged |
CNY 15,216.00 |
|
| RC226364L2 | Lenti ORF clone of Human RNA binding motif protein 20 (RBM20), mGFP tagged |
CNY 11,500.00 |
|
| RC226364L3 | Lenti ORF clone of Human RNA binding motif protein 20 (RBM20), Myc-DDK-tagged |
CNY 11,500.00 |
|
| RC226364L4 | Lenti ORF clone of Human RNA binding motif protein 20 (RBM20), mGFP tagged |
CNY 11,500.00 |
|
| RG226364 | RBM20 (tGFP-tagged) - Human RNA binding motif protein 20 (RBM20) |
CNY 14,416.00 |
|
| SC326360 | RBM20 (untagged)-Human RNA binding motif protein 20 (RBM20) |
CNY 23,660.00 |
