PCNXL3 (PCNX3) (NM_032223) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PCNXL3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC226440 representing NM_032223 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTCGCAGGTGTTGCAGATCCTGCGCCAGGGGGTGTGGGCCTCGCTCACCGGCGGTTGGTTCTTCG ACCCGCACCAGAGCACCTTCTCCAACTGCTTCCACCTCTATGTGTGGATCTTCCTGCTCATCTTTCCCTT CTTACTGTACATGGTCCTGCCTCCCAGCTTGATGGTGGCCGGCGTGTACTGCCTCGTGGTGGCTGTCATC TTTGCTACTATCAAGACTGTCAATTATCGGCTTCATGCCATGTTTGACCAGGGCGAGATCGTGGAGAAGC GCAGCTCTACCATGGGGGAGCTGGAGGAAGAGCCTGCCCAGGGGGACAGCAATCCACCCAGGGACCCCGG AGTGGAGATGACAGTGTTCCGGAAAGTCAGTTCCACACCCCCGGTGCGCTGCAGCTCCCAGCACTCTGTG TTTGGCTTCAACCAGGTCTCGGAGCTGCTGCCCCGAATGGAGGACTCTGGGCCCCTTAGAGACATCAAGG AGCTGGTGCGGGAGCAGGGCAGCAACAATGTGATCGTGACTTCTGCCGACCGAGAGATGCTGAAGCTCAG CTCGCAGGAGAAACTGATTGGAGACCTTCCCCAGACGCCTCCAGGGGCTGTCCCAGACCCCTCTCTTGCC AGTACAGACTCTTCAGAGCCTTCTCCCCTGGCTGGAGATGGAGCGCCCTGGAGTGGGAGCAGCATGGCTG ACACTCCCATGAGCCCCCTGCTGAAGGGGAGCCTCAGCCAGGAGCTGAGCAAGAGCTTCCTGACCCTGAC CCAGCCTGACCGGGCCCTGGTGAGGACCAGCAGTCGACGGGAACAACGCAGGGGGGCAGGTGGCTATCAG CCCCTTGACCGGCGGGGCTCAGGGGAGCCCACGCCCCAGAAAGCCGGCTCCTCAGACTCCTGCTTCAGCG GCACTGACAGGGAGACATTGAGCAGCTTCAAGAGTGAGAAGACCAACTCCACCCATCTGGACAGCCCCCC AGGGGGGCCAGCCCCTGAGGGCAGCGACACAGACCCACCCTCTGAGGCTGAGCTGCCTGCCTCACCAGAC GCCGGGGTCCCCTCAGATGACACGCTGCGTTCCTTTGACACGGTCATTGGAGCAGGGACGCCACCGGGCC TGGCTGAGCCGCTCCTGGTCGTGCGGCCCAAGGACTTGGCCCTGCTACGGCCTAGCAAACGGCAGCCACC CCTGCGAAGACACTCTCCACCTGGCCGTGCCCCTCGACGGCCCCTGCTTGAAGGTGGGGGCTTCTTTGAG GATGAAGACACTAGTGAGGGCAGTGAACTGAGCCCGGCCTCCAGTCTCCGATCGCAGCGCCGCTACAGTA CTGACAGCTCCTCTTCTACTTCCTGCTACTCCCCTGAGAGCTCCCGGGGTGCAGCAGGGGGACCCCGGAA GCGGAGGGCCCCCCATGGGGCTGAGGAGGGAACTGCTGTGCCCCCCAAGCGGCCATATGGGACCCAGCGG ACGCCTAGTACCGCCAGCGCTAAAACACATGCCCGTGTGCTGAGCATGGATGGGGCTGGGGGTGATGTTC TGAGGCCCCCACTGGCTGGCTGCAAGGCAGAGCTGGAGGCCCAGGTTGGGGTGGAGCAGGCTGCTAGTGA GCCTGTTGTGCTGCCTGCTGAGGCGCGAAGGGGACCCGCTGCCAACCAGCCCGGCTGGCGGGGGGAGCTG CAGGAGGAAGGTGCTGTGGGGGGAGCGGCCGAGGAGACTGGCAGGCGGGACCGCTCAAGCAGTGTGAGGC GGACCCAGGCCATTCGGAGACGCCACAATGCAGGCAGCAACCCCACCCCTCCAGCCTCTGTCATGGGCTC GCCGCCCAGCAGCCTGCAGGAAGCTCAGCGGGGCCGGGCTGCCTCCCACTCCCGGGCGCTGACGCTGCCC TCTGCGCTGCATTTCGCCTCTTCACTGTTGCTCACCCGGGCCGGTGCCAATGTGCATGAGGCCTGCACCT TTGATGACACTTCTGAGGGTGCTGTGCACTATTTCTACGATGAGAGCGGTGTGCGGCGTTCCTACACCTT TGGCCTGGCTGGAGGCGGCTACGAGAACCCTGTAGGGCAGCAAGGGGAGCAGACAGCTAATGGAGCCTGG GACCGACACTCGCATTCCTCCAGCTTCCACTCGGCTGATGTCCCTGAGGCTACAGGCGGCCTGAACCTGC TGCAGCCGAGGCCTGTGGTTCTGCAGGGCATGCAGGTGCGCCGGGTGCCCCTGGAGATCCCGGAGGAGCA GACACTGATGGAAGAAGCGCCACCCCGGGCCCAGCATAGTTACAAGTACTGGCTTCTCCCTGGCCGCTGG ACCTCTGTGCGCTATGAGCGGCTTGCCCTGCTGGCTCTGCTGGACCGGACGCGGGGAGTGCTGGAGAACA TCTTCGGCGTGGGCCTGAGCAGCCTTGTGGCCTTCCTGGGCTACCTGCTGCTGCTCAAGGGCTTCTTCAC TGACATCTGGGTCTTCCAGTTCTGCCTGGTCATCGCCTCCTGCCAGTACTCCTTGCTGAAGAGCGTGCAG CCTGATGCGGCGTCCCCCATGCACGGCCACAACTGGGTGATCGCGTACAGCCGTCCTGTCTACTTCTGCA TCTGCTGTCTGCTCATCTGGCTGCTGGACGCCCTGGGCTCAGCTCAGCCCTTCCCACCTGTCTCCCTCTA CGGCCTCACGCTCTTCTCTGCCTCCTTCTTCTTCTGTGCCCGAGACGTGGCCACTGTGTTCACCCTGTGC TTCCCCTTCGTCTTCCTCCTGGGCCTCCTGCCCCAGGTCAACACCTGCCTCATGTACCTGCTGGAGCAAA TAGATATGCACGGCTTCGGGGGCACAGCTGCCACCAGCCCGCTCACGGCAGTCTTCAGCCTCTCCCGCAG CCTGCTGGCTGCTGCCCTGCTCTACGGTTTCTGCCTTGGGGCCATCAAGACTCCGTGGCCAGAGCAGCAC GTCCCTGTCCTCTTCTCAGTCTTCTGTGGCCTCCTGGTGGCACTGTCCTACCACCTGAGCCGGCAGAGCA GCGACCCCACCGTGCTCTGGTCTCTGATCCGGAGCAAGCTGTTCCCTGAGCTGGAGGAGCGCAGCTTGGA GACAGCCCGGGCCGAGCCCCCGGACCCCTTGCCGGACAAGATGCGCCAGTCGGTGCGTGAGGTCTTGCAC TCCGACCTGGTGATGTGTGTGGTGATCGCCGTGCTCACCTTCGCCATCAGCGCCAGCACCGTCTTTATTG CCCTGAAGTCGGTGCTGGGTTTCGTGTTGTACGCACTGGCTGGGGCCGTGGGCTTCTTCACACATTACCT GCTGCCACAACTCCGCAAACAGCTGCCCTGGTTCTGCCTGTCACAGCCCGTGCTGAAGCCGCTGGAGTAC AGCCAGTATGAAGTGCGCGGTGCCGCCCAGGTGATGTGGTTTGAGAAGCTGTATGCTGGCCTGCAGTGCG TAGAGAAGTACCTCATCTACCCCGCCGTGGTGCTCAACGCCCTCACGGTGGACGCCCACACAGTCGTCAG CCACCCGGACAAGTACTGCTTCTACTGCCGGGCGCTGCTGATGACCGTGGCTGGGCTGAAGCTGCTGCGC TCAGCCTTCTGCTGCCCCCCACAGCAGTACCTGACGTTGGCCTTCACCGTCCTGCTCTTCCACTTTGACT ACCCGCGCCTCTCCCAGGGCTTTCTGCTTGACTACTTCCTCATGTCCCTGCTTTGCAGCAAGCTGTGGGA CTTGCTGTACAAGCTGCGTTTCGTGCTGACCTACATCGCGCCCTGGCAGATCACCTGGGGCTCGGCTTTC CACGCTTTTGCCCAGCCGTTTGCCGTGCCACACTCGGCCATGCTGTTCGTCCAGGCCCTGCTCTCGGGGC TCTTCTCCACGCCTCTCAACCCACTGCTAGGCAGTGCCGTCTTCATCATGTCCTACGCTCGGCCCCTCAA GTTCTGGGAGCGCGACTACAACACTAAACGTGTGGATCATTCCAACACCCGCCTGGTCACACAGCTGGAC AGGAACCCTGGCGCTGATGACAACAACCTCAACTCCATCTTCTATGAGCACTTGACACGTTCGCTGCAGC ACACACTGTGTGGGGACCTGGTGCTGGGCCGCTGGGGCAACTATGGCCCTGGTGACTGCTTCGTCCTGGC CTCTGACTACCTCAACGCCCTGGTGCACCTCATCGAGGTTGGCAATGGCCTCGTCACCTTCCAGCTGCGT GGCCTTGAGTTCCGGGGCACTTACTGCCAGCAGCGCGAGGTGGAGGCTATCACCGAGGGTGTGGAGGAGG ACGAGGGCTGTTGCTGCTGTGAACCTGGCCACCTGCCACGGGTCCTGTCCTTCAATGCTGCCTTTGGGCA GCGCTGGCTGGCTTGGGAGGTAACAGCCAGCAAGTACGTGCTGGAGGGCTATAGCATTAGTGACAATAAT GCTGCCTCCATGCTGCAGGTTTTCGACCTCCGCAAGATCCTCATCACCTACTATGTCAAGAGCATCATCT ACTACGTGAGCCGCTCACCAAAGCTGGAGGTGTGGCTCAGCCATGAGGGCATCACGGCAGCCCTGAGGCC TGTGCGGGTGCCCGGCTATGCCGACTCGGATCCCACCTTCTCGCTGAGTGTGGATGAGGACTATGACCTC CGCCTGTCTGGCCTCTCGCTGCCCTCCTTTTGTGCTGTGCACCTCGAGTGGATCCAGTACTGCGCCTCCC GGCGCAGCCAGCCCGTGGACCAGGATTGGAACTCCCCGCTGGTCACGCTGTGTTTTGGCCTGTGTGTGCT GGGCCGCCGGGCCCTGGGGACAGCCTCTCACAGCATGTCTGCAAGCCTGGAGCCCTTCCTCTACGGCCTG CACGCCCTGTTCAAGGGGGATTTTCGCATCACCTCCCCACGTGACGAGTGGGTCTTTGCCGACATGGACC TGCTTCACCGCGTTGTGGCGCCTGGGGTTCGCATGGCCCTCAAGCTTCACCAGGACCACTTCACGTCCCC AGATGAATATGAGGAGCCAGCAGCCCTATACGATGCCATTGCGGCCAACGAGGAGCGGCTGGTCATCTCA CATGAGGGTGACCCAGCATGGCGCAGCGCCATCCTCAGCAACACGCCCTCCCTGCTGGCGCTGCGCCATG TCCTGGATGATGCCTCCGACGAGTACAAGATCATCATGCTCAACCGGCGCCACCTCAGCTTCCGAGTCAT CAAGGTGAACCGGGAGTGCGTGCGCGGCCTGTGGGCCGGGCAGCAGCAGGAGCTGGTGTTCCTGCGCAAC CGCAACCCCGAGCGTGGCAGCATCCAGAACGCCAAGCAGGCGCTTCGCAACATGATCAACTCCTCCTGTG ACCAGCCGCTGGGCTACCCCATCTACGTGTCGCCTCTCACCACCTCGCTGGCTGGCAGCCACCCCCAGCT ACGGGCACTGTGGGGTGGCCCCATCAGCCTGGGTGCCATTGCCCACTGGCTCCTGCGCACCTGGGAGAGG CTTCACAAGGGCTGTGGCGCCGGCTGCAATAGTGGCGGGAACGTGGATGATTCAGACTGTAGTGGGGGCG GTGGCCTGACCTCCCTCAGCAATAACCCCCCCGTGGCACACCCCACACCTGAGAACACGGCAGGCAATGG TGACCAACCCCTCCCACCAGGCCCTGGCTGGGGGCCGCGGTCCTCCCTGAGTGGCTCTGGTGATGGGCGG CCCCCACCTCTGCTGCAGTGGCCTCCCCCTCGGCTCCCTGGACCACCCCCTGCATCGCCTATCCCCACAG AGGGTCCCCGGACCTCACGGCCCCCTGGCCCGGGTCTCCTCAGTTCTGAGGGCCCCAGTGGAAAGTGGAG CCTGGGGGGCCGGAAGGGGCTGGGAGGATCTGACGGGGAGCCAGCCTCAGGGAGCCCCAAAGGAGGTACC CCCAAATCTCAGGCGCCTCTAGACCTCAGCCTCAGCCTCAGCCTCAGCCTCAGCCCCGATGTCAGCACTG AGGCCTCACCCCCCAGAGCTTCCCAGGACATTCCTTGCTTGGACAGCAGTGCCCCTGAGAGTGGCACACC TATGGGTGCCCTGGGCGACTGGCCTGCCCCTATTGAGGAGCGTGAGAGCCCGGCAGCCCAGCCCCTGCTG GAACACCAGTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC226440 representing NM_032223 Red=Cloning site Green=Tags(s) MGSQVLQILRQGVWASLTGGWFFDPHQSTFSNCFHLYVWIFLLIFPFLLYMVLPPSLMVAGVYCLVVAVI FATIKTVNYRLHAMFDQGEIVEKRSSTMGELEEEPAQGDSNPPRDPGVEMTVFRKVSSTPPVRCSSQHSV FGFNQVSELLPRMEDSGPLRDIKELVREQGSNNVIVTSADREMLKLSSQEKLIGDLPQTPPGAVPDPSLA STDSSEPSPLAGDGAPWSGSSMADTPMSPLLKGSLSQELSKSFLTLTQPDRALVRTSSRREQRRGAGGYQ PLDRRGSGEPTPQKAGSSDSCFSGTDRETLSSFKSEKTNSTHLDSPPGGPAPEGSDTDPPSEAELPASPD AGVPSDDTLRSFDTVIGAGTPPGLAEPLLVVRPKDLALLRPSKRQPPLRRHSPPGRAPRRPLLEGGGFFE DEDTSEGSELSPASSLRSQRRYSTDSSSSTSCYSPESSRGAAGGPRKRRAPHGAEEGTAVPPKRPYGTQR TPSTASAKTHARVLSMDGAGGDVLRPPLAGCKAELEAQVGVEQAASEPVVLPAEARRGPAANQPGWRGEL QEEGAVGGAAEETGRRDRSSSVRRTQAIRRRHNAGSNPTPPASVMGSPPSSLQEAQRGRAASHSRALTLP SALHFASSLLLTRAGANVHEACTFDDTSEGAVHYFYDESGVRRSYTFGLAGGGYENPVGQQGEQTANGAW DRHSHSSSFHSADVPEATGGLNLLQPRPVVLQGMQVRRVPLEIPEEQTLMEEAPPRAQHSYKYWLLPGRW TSVRYERLALLALLDRTRGVLENIFGVGLSSLVAFLGYLLLLKGFFTDIWVFQFCLVIASCQYSLLKSVQ PDAASPMHGHNWVIAYSRPVYFCICCLLIWLLDALGSAQPFPPVSLYGLTLFSASFFFCARDVATVFTLC FPFVFLLGLLPQVNTCLMYLLEQIDMHGFGGTAATSPLTAVFSLSRSLLAAALLYGFCLGAIKTPWPEQH VPVLFSVFCGLLVALSYHLSRQSSDPTVLWSLIRSKLFPELEERSLETARAEPPDPLPDKMRQSVREVLH SDLVMCVVIAVLTFAISASTVFIALKSVLGFVLYALAGAVGFFTHYLLPQLRKQLPWFCLSQPVLKPLEY SQYEVRGAAQVMWFEKLYAGLQCVEKYLIYPAVVLNALTVDAHTVVSHPDKYCFYCRALLMTVAGLKLLR SAFCCPPQQYLTLAFTVLLFHFDYPRLSQGFLLDYFLMSLLCSKLWDLLYKLRFVLTYIAPWQITWGSAF HAFAQPFAVPHSAMLFVQALLSGLFSTPLNPLLGSAVFIMSYARPLKFWERDYNTKRVDHSNTRLVTQLD RNPGADDNNLNSIFYEHLTRSLQHTLCGDLVLGRWGNYGPGDCFVLASDYLNALVHLIEVGNGLVTFQLR GLEFRGTYCQQREVEAITEGVEEDEGCCCCEPGHLPRVLSFNAAFGQRWLAWEVTASKYVLEGYSISDNN AASMLQVFDLRKILITYYVKSIIYYVSRSPKLEVWLSHEGITAALRPVRVPGYADSDPTFSLSVDEDYDL RLSGLSLPSFCAVHLEWIQYCASRRSQPVDQDWNSPLVTLCFGLCVLGRRALGTASHSMSASLEPFLYGL HALFKGDFRITSPRDEWVFADMDLLHRVVAPGVRMALKLHQDHFTSPDEYEEPAALYDAIAANEERLVIS HEGDPAWRSAILSNTPSLLALRHVLDDASDEYKIIMLNRRHLSFRVIKVNRECVRGLWAGQQQELVFLRN RNPERGSIQNAKQALRNMINSSCDQPLGYPIYVSPLTTSLAGSHPQLRALWGGPISLGAIAHWLLRTWER LHKGCGAGCNSGGNVDDSDCSGGGGLTSLSNNPPVAHPTPENTAGNGDQPLPPGPGWGPRSSLSGSGDGR PPPLLQWPPPRLPGPPPASPIPTEGPRTSRPPGPGLLSSEGPSGKWSLGGRKGLGGSDGEPASGSPKGGT PKSQAPLDLSLSLSLSLSPDVSTEASPPRASQDIPCLDSSAPESGTPMGALGDWPAPIEERESPAAQPLL EHQY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_032223
ORF Size	6102 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_032223.2, NP_115599.2
RefSeq ORF	6105 bp
Locus ID	399909
UniProt ID	Q9H6A9
MW	221.9 kDa

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